Production of granticin by Streptomyces spp isolated from soil samples
DOI:
https://doi.org/10.36320/ajb/v16.i1.13611Keywords:
Streptomyces ,granticin ,antibacterial spectrum.Abstract
Granaticin, a benzoisochromanequinone (BIQ) produced by streptomyces sp . In this study. 103 soil samples were collected from seven Iraqi provinces. Ten isolates were obtained that inhibit pathogenic bacteria. The Streptomyces isolates are identified by microscopy and morphology. These isolates were diagnosed genetically through the 16s rRNA gene. Five isolates were sent for sequence analysis. After that, the Streptomyces isolates that produced granticin were searched for by means of By changing the color of the producer isolate from red to violet when adding the base NaOH and genetically through the orf-gra 11 gene that encoding to a dTDP-glucose dehydratase enzyme granticin(500bp) by conventional PCR., one isolate was obtained that produced granticin Streptomyces RS18. By examining the inhibition activity of the isolates, the isolate RS18 showed effective inhibition against gram -positive bacteria, and there was no activity for gram-negative bacteria. Further chemical characterization of the local iraqi granticin is very usefull in its pharmaceutical companies.
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