Genotypic And Phenotypic Surveillance Of Multidrug Resistance Of Hypermucoid K. Pneumoniae Among Clinical Isolates In AL Najaf City /Iraq
DOI:
https://doi.org/10.36320/ajb/v15.i3.14615Keywords:
K. pneumoniae, hypervirulent, Antibiotics resistanceAbstract
Hypervirulent Klebsiella pneumoniae strains are the major cause of liver abscesses ,diabetes foot ulcers and other infections throughout Iraq, and these strains are usually antibiotics susceptible. Recently, hypervirulent K. pneumoniae isolates have emerged due to acquiring antimicrobial resistance determinants or the transfer of a virulence plasmid into a classic isolates ,.Klebsiella pneumoniae is responsible for hospital- and community-acquired infections. This study aimed to determine the prevalence of hyper irulence K. pneumoniae and investigate the antibiotics resistance profile among clinical specimens at Al Najaf Hospitals in/ Iraq, and detect the genes for molecular identification of K. pneumoniae in comparison with phenotypic and biochemical methods. In total, 100 clinical specimens were collected from patients infected with K. pneumoniae, between January 2023 and May 2023 from both sexes males (38) and Females (62) with age ranged between (20-70) years. The Hypervirulent Klebsiella pneumoniae isolates were cultured on general and selective media as (Blood agar and MacConkey agar and CHROM agar), and confirmed by using Vitek-2 system. The isolates were reported with high resistance towards various types of antibiotics, especially penicillins and cephalosporins. In contrast, K. pneumoniae showed very low resistance to imipenem and amikacin. According to the results the range of multidrug-resistant K. pneumoniae isolates in this study was estimated at 100%.
Downloads
References
Hao C, Abhilasha K, Duy P, Christine J. A (2015) high- resolution genomic analysis of multidrugresistant hospital outbreaks of Klebsiella pneumoniae. You have full text access to this OnlineOpen article EMBO Molecular Medicine, 7:3.
Chon J, Kim Y.( 2015) Prevalence and characterization of extended-spectrum-β- lactamase-producing Escherichia coli, and Klebsiella pneumoniae, in ready-to-eat vegetables. Int. J. Food Microbiol,207: 83–86.
Ravichitra KN, P Hema, S . Subbarayudu (2014). Isolation and antibiotic sensitivity of Klebsiella pneumoniae from pus, sputum and urine samples,3(3) :115-119
Coyle M. B. (2005). Manual of Antimicrobial Susceptibility Testing; American for Microbiology Press, Washington D.C. pp:25-39
Lewis, J.S., Herraera M., Wickers B., Patterson J.E. and Jorgensen J.H. (2007). First report of the emergency of CTX-M- type extended spectrum beta lactamases (ESBLs) as the predominant ESBL isolated in a US healthcare system. Antimicrobe. Agents Chemother.51:4015-4021
Mariya S., Sunil S Hatkar. (2015). Antimicrobial susceptibility profile of urinary isolates of Escherichia Coli and Klebsiella Pneumoniae International Journal of Health Sciences and Research (www.ijhsr.org) .
Coyle M. B. (2005). Manual of Antimicrobial Susceptibility Testing; American for Microbiology Press, Washington D.C. pp:25-39
Piddock, L.J.V. (2006). Clinically relevant chromosomally encoded multi-drug resistance efflux pumps in bacteria. Clin. Microbiol. Rev., 19(2): 382-402
Ammar, M. H., & AL-Quraishi, Z. H. O. (2018). phenotypic detection of some virulence factors in salmonella typhi carrier associated with gall bladder chronic infection. Plant Archives, 18(1), 1081-1087.
Sara thbabu R, Ramani TV, Bhaskara rao K, Panda S. (2012). Antibiotic susceptibility pattern of Klebsiella pneumoniae isolated from sputum, urine and pus samples. J Pharm and Biolo Sci.1(2):4-9.
Tsukada H, Cho H, Kimura Y, Tetsuka T, Nakajima H, Ito K, Sakai K, (2012). Retrospective investigation of the clinical effects of tazobactam/piperacillin and sulbactam/ampicillin on aspiration pneumonia.
Liu B, Yi Y, Wang Q, Woo PCY, Tan L, Jing H, et al. (2013).Analysis of drug resistance determinants in Klebsiella pneumoniae isolates fromatertiary- carehospitalinBeijing,China.7:42280.
.Almogbel, M., Altheban, A., Alenezi, M., Al-Motair, K., Menezes, G. A., Elabbasy, M., Hammam, S., Hays, J. P., & Khan, M. A. (2021). CTX-M-15 positive Escherichia coli and Klebsiella pneumoniae outbreak in the neonatal intensive care unit of a maternity hospital in Ha’il, Saudi Arabia. Infection and Drug Resistance, 2843–2849.
.Jubran, A. S., Al-Zamely, O. M., & Al-Ammar, M. H. (2020). A study of iron oxide nanoparticles synthesis by using bacteria. Int. J. Pharm. Qual. Assur, 11(01), 01-08.
AL-Huchaimi, S. H. K., Jassim, A., AL-Hadad, M. T. S., & AL-Ammar, M. H. (2018). detection of pathogenicity markers produced by pseudomonas aeruginosa causing skin infection. Plant Archives, 18(1), 621-626.
AL-Kraety, I. A. A., & Al-Ammar, M. (2017). Relation of class1 integron gene with multi-drug resistance salmonella typi isolates. Pak. J. Biotechnol. Vol, 14(4), 537-541.
Al-Ammar, M., & Al Maghathry, A. S. (2017). Molecular detection of antibiotic resistance genes of Acenitobacter bumannii. Pakistan Journal of Biotechnology, 14(4), 777-783.
Ammar, M. H., & AL-Quraishi, Z. H. O. (2018). phenotypic detection of some virulence factors in salmonella typhi carrier associated with gall bladder chronic infection. Plant Archives, 18(1), 1081-1087.
Al, A. P. D. H. A., Dahhan, M. C. A., Ali, M., & Al-Ammer, M. Phenotypic and Genotypic Characterization of Salmonella Typhi Virulence Factors Isolated From Patients with Typhoid Fever in Najaf Province/Iraq.
Al-Muhanna, M. R., & Al-Ammar, M. (2020). Molecular Detection of Antibiotics Resistance Genes in Burkholderia cepecia isolated from Diabetic foot infection. Indian Journal of Forensic Medicine & Toxicology, 14(2), 2188-2192.
Alnasrawy, W. D., & AL-Aammar, M. H. (2021). A Molecular Study with A Comparison of the Odds of Diagnostic Methods For Burkholderia Cepacia Bacteria Isolated from Patients with Diabetic Foot Ulcer. Indian Journal of Forensic Medicine & Toxicology, 15(3), 4603-4609.
AL-Ammar, M. H., & AL-Quraishi, Z. H. O. (2018). phenotypic detection of some virulence factors in salmonella typhicarrier associated with gall bladder chronic infection. Plant Archives, 18(1), 1081-1087.
AL-Quraishi, Z. H. O., & AL-Amm, P. H. Genotypic Study of Some Virulence Factors in Salmonella Typhi Carrier Associated with Gall Bladder Chronic Infection.
Al-Sadawi, A. A., Ammar, M., & Tuwaij, N. S. (2017). Viral agent that causing diarrhoea among children in Al-Najaf province, Iraq. World J Pharma Res, 6(8), 1-11.
Al-Ammar, M. H. (2012). Detection of extended-spectrum β-lactamases in Aeromonas hydrophila isolated from stool samples in the Babylon Province Iraq. Int Res J Microbiol, 3(9), 317-21.
Abid, A. J., Almashta, S. A., & Ismail, R. J. (2020). Prevalence of Klebsiellapneumoniae in Renal Failure Patients Using Genes Specific Primers. Indian Journal of Public Health Research & Development, 11(4).
Ali, F., Kamal, S., Shakeela, Q., & Ahmed, S. (2021). Extended-spectrum and Metallo-beta lactamase enzymes mediated resistance in Pseudomonas aeruginosa in clinically isolated specimens. Kuwait Journal of Science, 48(2).
Hassan Al-Saffar, V. (2019). Extended Spectrum β-lactamases (ESBL) producing Escherichia coli (E.coli). Al-Kufa University Journal for Biology, 11(1). Retrieved from https://journal.uokufa.edu.iq/index.php/ajb/article/view/8039Amin.
Hasan Al-Fanharawi, A., Jasim Al-Shamarti, M., & Chayad Al-Janahi, H. (2023). The Role Of OprD Gene In The Carbapenems Resistance In Pseudomonas aeruginosa Isolated From Burns Infection In Al-Najaf Province. Al-Kufa University Journal for Biology, 14(3), 8–16. https://doi.org/10.36320/ajb/v14.i3.11150.
M. Abd Asada , M., & Aziz Mahal Al-amri, N. (2021). Molecular identification and Virulence factors of Pseudomonas aeruginosa isolated from operation hall. Al-Kufa University Journal for Biology, 13(2), 3946. https://doi.org/10.36320/ajb/v13.i2.11758.
Zainab M.K.AL-Sendi, Ilham A. Bunyan.(2023) Molecular detection of some β-lactamase genes among K.pneumonia 258.Journal of Survey in Fisheries Sciences.10(3S)3245-3254
Downloads
Published
Versions
- 2024-01-03 (3)
- 2023-12-31 (2)
- 2023-12-31 (1)
How to Cite
Issue
Section
License
Copyright (c) 2023 Ali Hussain Hmood, Mahdi H.M. Alammar
This work is licensed under a Creative Commons Attribution 4.0 International License.
which allows users to copy, create extracts, abstracts, and new works from the Article, alter and revise the Article, and make commercial use of the Article (including reuse and/or resale of the Article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made and the licensor is not represented as endorsing the use made of the work.
