A Molecular identification of Staphylococcus aureus strains isolated from diabetic foot ulcers based on 16SrRNA
DOI:
https://doi.org/10.36320/ajb/v16.i2.15520Keywords:
diabetes, ulcer, PCR, STAPHYLOCOCCUS AUREUS, 16SrRNAAbstract
Introduction: Diabetic foot ulcer (DFU) is the most serious diabetic complication. Gangrene is caused by successive bacterial infections invading diabetic wounds and may lead to limb amputation for the diabetic patient. Staphylococcus aureus (S. aureus) is one of the most prevalent bacterial pathogens recovered from diabetic foot infections (DFIs). It is found worldwide and is a leading cause of disease. Many methods have been used for identifying the associated strains in clinical specimens. Sequencing by 16SrRNA methods is achieved in the identification. The current study aimed to detect s aureus isolated from Diabetic foot ulcers by 16SrRNA.
Methodology: One hundred clinical samples were collected from patients with diabetic foot ulcers at Faida Primary Health Center, Mosul City, Iraq. The bacterium was identified using Gram stain, colony morphology, biochemical tests, selective differential media, and molecular analyses.
Results: A total of 60 S. aureus isolates were obtained out of a total of 100. In the molecular study, 10 isolates from 60 were selected for PCR assay. Selected isolates were confirmed by PCR assay that successfully produced amplified product 16SrRNA gene with size 164 bp.
Conclusions: Our results support the recent view that gram-positive organisms, depending on the geographical location, may be predominantly DFIs.
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