Relationship of biofilm strength levels in Candida albicans isolates with the type of clinical specimens
علاقة مستويات قوة الغشاء الحيوي في عزلات المبيضات البيضاء بنوع العينات السريرية
DOI:
https://doi.org/10.36320/ajb/v16.i2.16560Keywords:
candida albicans, The virulence factors, biofilm formation, immunocompromised patients.Abstract
The virulence factors in C. albicans such as biofilm formation, germ tube, adhesion, hydrolytic enzymes, such as lipases, proteases, phospholipases, and hemolysin and catalase enzyme that allow successful colonization and infection of the host under suitable predisposing conditions. A total of 151 clinical specimens were collected from immunocompromised patients suffering from underlying diseases included cancer, diabetes, and thalassemia. All the collected specimens were cultured on yeast selective media. From the total clinical specimens 111 (73.50%) specimens were positive for yeast growth, among them the most predominant species was C. albicans at 64 (57.65%). There was a difference in the prevalence of yeast species isolates according to the patient’s gender, which it was higher in females 76 (68.46%) than in males 35 (31.53%), The virulence factors results showed that all isolates belonging to the species C.albicans form the germ tube when incubated at a temperature of 37°C for 3 hours in 0.5 ml of human serum while all the isolates of C. albicans showed a positive result to adhesion test after incubation for 1h at 37Cº The test results showed that from 64 C. albicans isolates, there were 16 (25%) strong biofilm producers, 6 (14.28%) were considered as weak biofilm producers. The studies described here form the basis for investigations into the mechanisms of Candida biofilm formation and provide the means to design novel therapies for biofilm-based infections.
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