The Crocus Sativus Stigma Extract as Anticancer for SKOV-3 Cell Line
DOI:
https://doi.org/10.36320/ajb/v16.i2.16687Keywords:
Crocus Sativus Stigma, Anticancer, SKOV-3 Cell Line, and fluorescent dye Rhodamine (Rh123).Abstract
Cancer is an uncontrolled growth and division of abnormal cells with the capacity to spread to other body regions (metastasis). The aim of this study is to investigate the effect of saffron aqueous extract an anticancer on the ovarian cancer the material and method included preparation the saffron aqueous extract and SKOV-3 after maintained of cell cultures in RPMI-1640 supplemented with 10% Fetal bovine serum, penicillin and streptomycin. Next, two methods were used to investigate the impact of saffron. One of these methods involves measuring the impact of saffron on the mitochondrial activity of SKOV-3 cells using the fluorescent dye Rhodamine (Rh123). Furthermore, the activation statuses of p53 and caspase-3 were evaluated using a fluorescein caspase-3 and p53 staining kit. Eventually, the results were promising because the saffron extract caused damage in mitochondria and a significant increase by caspase-3 and p53 that caused to apoptosis SKOV-3 cells without effect on normal cells. Finally, the usage of saffron supplements in addition to established treatment procedures may benefit people with ovarian cancer since saffron extract triggers cancer cell apoptosis through several indications that manifest differently.
Downloads
References
Aboumsallem, J. P., Moslehi, J., & de Boer, R. A. (2020). Reverse cardio‐oncology: cancer development in patients with cardiovascular disease. Journal of the American Heart Association, 9(2), e013754. DOI: https://doi.org/10.1161/JAHA.119.013754
Levine, A. J. (2020). p53: 800 million years of evolution and 40 years of discovery. Nature Reviews Cancer, 20(8), 471-480. DOI: https://doi.org/10.1038/s41568-020-0262-1
Gambelli, A., Nespolo, A., Rampioni Vinciguerra, G. L., Pivetta, E., Pellarin, I., Nicoloso, M. S., ... & Baldassarre, G. (2024). Platinum-induced upregulation of ITGA6 promotes chemoresistance and spreading in ovarian cancer. EMBO Molecular Medicine, 1-31. DOI: https://doi.org/10.1038/s44321-024-00069-3
Feeney, L., Harley, I. J., McCluggage, W. G., Mullan, P. B., & Beirne, J. P. (2020). Liquid biopsy in ovarian cancer: Catching the silent killer before it strikes. World Journal of Clinical Oncology, 11(11), 868. DOI: https://doi.org/10.5306/wjco.v11.i11.868
Bai, M. Y., Yu, M. H., Wang, T. T., Chen, S. H., & Wang, Y. C. (2021). Plate-like alginate microparticles with disulfiram–SPIO–coencapsulation: an in vivo study for combined therapy on ovarian cancer. Pharmaceutics, 13(9), 1348. DOI: https://doi.org/10.3390/pharmaceutics13091348
Okyay, A. G., Kaplan, H. M., Asil, H., & Singirik, E. (2020). Saffron induces Apoptosis in Ovarian Cancer cell via MAPK and AKT/mTOR Pathways. PROGRESS IN NUTRITION, 22.
Lambrianidou, A., Koutsougianni, F., Papapostolou, I., & Dimas, K. (2020). Recent advances on the anticancer properties of saffron (Crocus sativus L.) and its major constituents. Molecules, 26(1), 86. DOI: https://doi.org/10.3390/molecules26010086
Dailah, H. G. (2022). Carthamus tinctorius L. Inhibits Proliferation of Lung Cancer A549 Cells and Tender’s Mitochondrial Protection. A A (treated), 490(630), 490. DOI: https://doi.org/10.5530/ijper.56.3.129
Naeem, A., Hu, P., Yang, M., Zhang, J., Liu, Y., Zhu, W., & Zheng, Q. (2022). Natural products as anticancer agents: Current status and future perspectives. Molecules, 27(23), 8367. DOI: https://doi.org/10.3390/molecules27238367
Ibrahim, A. A., Kareem, M. M., Al-Noor, T. H., Al-Muhimeed, T., AlObaid, A. A., Albukhaty, S., ... & Sahib, U. I. (2021). Pt (II)-thiocarbohydrazone complex as cytotoxic agent and apoptosis inducer in Caov-3 and HT-29 Cells through the P53 and caspase-8 pathways. Pharmaceuticals, 14(6), 509. DOI: https://doi.org/10.3390/ph14060509
Bokhari, S. S., Ali, T., Naeem, M., Hussain, F., & Nasir, A. (2024). Recent advances in nanoformulation-based delivery for cancer immunotherapy. Nanomedicine, (0).
Abd Rashid, N., Abd Halim, S. A. S., Teoh, S. L., Budin, S. B., Hussan, F., Ridzuan, N. R. A., & Jalil, N. A. A. (2021). The role of natural antioxidants in cisplatin-induced hepatotoxicity. Biomedicine & Pharmacotherapy, 144, 112328. DOI: https://doi.org/10.1016/j.biopha.2021.112328
Jabir, M., Sahib, U. I., Taqi, Z., Taha, A., Sulaiman, G., Albukhaty, S., ... & Rizwana, H. (2020). Linalool-loaded glutathione-modified gold nanoparticles conjugated with CALNN peptide as apoptosis inducer and NF-κB translocation inhibitor in SKOV-3 cell line. International Journal of Nanomedicine, 9025-9047. DOI: https://doi.org/10.2147/IJN.S276714
Al-Ziaydi, A. G., Al-Shammari, A. M., Hamzah, M. I., Kadhim, H. S., & Jabir, M. S. (2020). Hexokinase inhibition using D-Mannoheptulose enhances oncolytic newcastle disease virus-mediated killing of breast cancer cells. Cancer Cell International, 20, 1-10. DOI: https://doi.org/10.1186/s12935-020-01514-2
Al-Ziaydi, A. G., Hamzah, M. I., Al-Shammari, A. M., Kadhim, H. S., & Jabir, M. S. (2020, December). The anti-proliferative activity of D-mannoheptulose against breast cancer cell line through glycolysis inhibition. In AIP Conference Proceedings (Vol. 2307, No. 1). AIP Publishing. DOI: https://doi.org/10.1063/5.0032958
Bahjat, H. H.; Ismail, R. A.; Sulaiman, G. M.; Jabir, M. S., Magnetic field-assisted laser ablation of titanium dioxide nanoparticles in water for anti-bacterial applications. Journal of Inorganic and Organometallic Polymers and Materials 2021, 1-8. DOI: https://doi.org/10.21203/rs.3.rs-176836/v1
Al-Omar, M. S., Jabir, M., Karsh, E., Kadhim, R., Sulaiman, G. M., Taqi, Z. J., ... & Mohammed, S. A. (2021). Gold nanoparticles and graphene oxide flakes enhance cancer cells’ phagocytosis through granzyme-perforin-dependent biomechanism. Nanomaterials, 11(6), 1382. DOI: https://doi.org/10.3390/nano11061382
Sarfarazi, M., Jafari, S. M., Rajabzadeh, G., & Galanakis, C. M. (2020). Evaluation of microwave-assisted extraction technology for separation of bioactive components of saffron (Crocus sativus L.). Industrial Crops and Products, 145, 111978. DOI: https://doi.org/10.1016/j.indcrop.2019.111978
Naim, N., Guirrou, I., Fauconnier, M. L., Hafida, H., Tahiri, A., Madani, I., ... & Ennahli, S. (2023). Chemical, biochemical and volatile profiles of saffron (Crocus sativus L.) from different growing areas of Morocco. JSFA Reports, 3(5), 233-247. DOI: https://doi.org/10.1002/jsf2.114
Esmaealzadeh, D., Ghalibaf, A. M., Rad, M. S., Rezaee, R., Razavi, B. M., & Hosseinzadeh, H. (2023). Pharmacological effects of Safranal: An updated review. Iranian Journal of Basic Medical Sciences, 26(10), 1131.
Bokhari, S. S., Ali, T., Naeem, M., Hussain, F., & Nasir, A. (2024). Recent advances in nanoformulation-based delivery for cancer immunotherapy. Nanomedicine, (0). DOI: https://doi.org/10.1080/17435889.2024.2343273
Raeeszadeh-Sarmazdeh, M., Do, L. D., & Hritz, B. G. (2020). Metalloproteinases and their inhibitors: potential for the development of new therapeutics. Cells, 9(5), 1313. DOI: https://doi.org/10.3390/cells9051313
Shakeri, M., Tayer, A. H., Shakeri, H., Jahromi, A. S., Moradzadeh, M., & Hojjat-Farsangi, M. (2020). Toxicity of saffron extracts on cancer and normal cells: A review article. Asian Pacific journal of cancer prevention: APJCP, 21(7), 1867. DOI: https://doi.org/10.31557/APJCP.2020.21.7.1867
Othman, R., Hatta, F. A. M., Hassan, N. M., & Kamoona, S. (2020). Characterization of carotenoids content and composition of saffron from different localities. J. Pharm. Nutr. Sci, 10, 34-40. DOI: https://doi.org/10.29169/1927-5951.2020.10.01.6
Hatziagapiou, K., Nikola, O., Marka, S., Koniari, E., Kakouri, E., Zografaki, M. E., ... & Tarantilis, P. A. (2022). An in vitro study of saffron carotenoids: the effect of crocin extracts and dimethylcrocetin on cancer cell lines. Antioxidants, 11(6), 1074. DOI: https://doi.org/10.3390/antiox11061074
Gill, N. B., Dowker-Key, P. D., Hedrick, M., & Bettaieb, A. (2024). Unveiling the Role of Oxidative Stress in Cochlear Hair Cell Death: Prospective Phytochemical Therapeutics against Sensorineural Hearing Loss. International Journal of Molecular Sciences, 25(8), 4272. DOI: https://doi.org/10.3390/ijms25084272
Bertheloot, D., Latz, E., & Franklin, B. S. (2021). Necroptosis, pyroptosis and apoptosis: an intricate game of cell death. Cellular & molecular immunology, 18(5), 1106-1121. DOI: https://doi.org/10.1038/s41423-020-00630-3
Downloads
Published
Issue
Section
License
Copyright (c) 2024 Rusul Hammoodi Hasan Kabla , Jinan M. Abed-al-Zahra
This work is licensed under a Creative Commons Attribution 4.0 International License.
which allows users to copy, create extracts, abstracts, and new works from the Article, alter and revise the Article, and make commercial use of the Article (including reuse and/or resale of the Article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made and the licensor is not represented as endorsing the use made of the work.
