Biosynthesis Silver nanoparticles by using Supernatant staphylococcus epidermidis and antibacterial Activity of resistant pathogenic bacteria
Biological activities of silver nanoparticles
DOI:
https://doi.org/10.36320/ajb/v16.i2.16689Keywords:
Supernatant bacterial, Staphylococcus epidermidis, Silver nanoparticles, GC-Ms, absorbanceAbstract
Nanotechnology is a contemporary technological field that emerged towards the conclusion of the twentieth century. This technique gained widespread recognition and played a pivotal role due to its capacity to effectively address the challenges encountered by medications, namely by enhancing absorption and disintegration. Furthermore, the importance of stability and accurate distribution of therapy to the intended site has been emphasized by (1) ; (2). Nanotechnology refers to the manipulation and fabrication of particles that are smaller than 100 nanometers in size (3) in order to enhance the surface area. The area-to-volume ratio facilitates chemical interactions between individual atoms (4). The present work used a bacterial filtrate derived from Staphylococcus epidermidis bacterium and silver nitrate. The GC-Ms analysis of the bacterial isolate extract identified many highly concentrated active chemicals, including methyl phenyl sulfoxide, 2, 3 Friedelin, and 1, 3, 5-triphenylcyclohexane.
The UV-visible light spectrum of silver nanoparticles showed the highest absorbance peak at wavelength 416. X-ray diffraction (XRD) analysis showed four peaks at levels 111, 200, 220, and 311. The Fourier Transform Infrared Spectroscopy (FTIR) spectrum of the extracellular bacterial filtrate and the resulting silver particles showed several bands in the range 500-4000 cm-1, which indicate O-H groups for alcoholic and phenolic compounds, N-H groups for amino acids, C=C for alkanes, and S= O belongs to sulfur compounds. Field-Emission Scanning Electron Microscope (FSEM) showed that silver nanoparticles are located within a nanoscale orbit. Their sizes reached 37.78-61.29 nanometers, with an average of 19.75 nanometers. Energy-dispersive X-ray spectroscopy (EDX), which accompanies the scanning electron microscope examination, recorded the weight percentage of silver as equal to 88%.
Silver nanoparticles manufactured from the extract of Staphylococcus epidermidis bacteria were effective against multi-antibiotic-resistant pathogenic isolates. Staphylococcus aureus bacteria were more resistant, with an average diameter of inhibition of 11.11 mm. While Pseudomonas aeruginosa bacteria were more sensitive, with an average diameter of inhibition of 14.57 mm, the concentration of 200 µg/ml was the most effective.
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