Molecular Study of Virulence Factors of Candida spp. That Isolated from Cancer Patients
DOI:
https://doi.org/10.36320/ajb/v16.i2.18013Keywords:
Candida, Cancer, Multiplex- PCR technique, Virulence factorsAbstract
study of microbes and cancer is considered one of the challenges in sustainable development. Candida is an opportunistic pathogenic fungus, which is inclined to infect the host with faulty immune function involving patients with cancer. A growing many of research have shown that Candida infection rise the host's vulnerability to cancer such as colorectal, gastric, and oral cancer. Aim: This investigation demonstrates the isolation and identification of Candida spp. from cancer patients of Middle Euphrates Cancer Center in Al- Najaf governorate, Method: (51) blood specimens were collected for the preparation of serum.
The serum is cultured on SDA media and the culture is examined for microbial growth, such as culture on SDA media, Chromo agar, microscopic examination, and then by Multiplex- PCR technique. Results: The current investigate showed infection percentage with opportunistic microorganisms was demonstrated as follows: C. tropicalis appeared in 40% in males and 0% in females , C. albicans appeared 10% in males, 5% in females, C. albicans appeared in cancer 10% in males, 5% in females, C. guilliermondii appeared in cancer 0% in males and 2% in females, C. parapsilosis and C. dubliniensis didn’t appear in cancer. In cancer isolates the ALS1 gene was found strongly in C. albicans, and don’t found in C. guillermondii, and weakly appeared in C. tropicalis, and the PLB1gene was found weakly in C. albicans, and don’t found in C. guillermondii, and C. tropicalis. Conclusion: In the normal state, the blood is sterile, but when the immunity is suppressed or weakened as a result of a specific symptom or disease, the whole body and the blood are also vulnerable to any opportunistic microbe that grows and multiplies with it. So the presence of microbes in the blood is a strong indicator of weak immunity in cancer patients to the point of penetration of Candida into the blood. We concluded from our current study that some pathogenic microbes can be isolated from the sera of cancer patients, as some types of Candida were isolated, which include C.albicans, C. tropicalis, ,and C.guillermondii.Our results of this study indicated the capacity of some of Candidia spp. to produced phospholipase enzyme. Not all C. albicans isolates was positive for phospholipase production, we also conclude that some genes of virulence factors don’t necessary to express and Candida can be pathogenic without it.
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