Bioremediation potential of some heavy metals by bacterial strains isolated from municipal wastewater
DOI:
https://doi.org/10.36320/ajb/v17.i2.19425Keywords:
Bioremediation Heavy metal WastewaterAbstract
Standard guidelines were used to assess the physicochemical characteristics of the effluent samples from municipal sewage treatment plant in Diwaniyah city. Physicochemical parameters assessed include temperature that was 29°C, PH value was 27.7, biochemical oxygen demand concentration was 449, chemical oxygen demand was 1470, BOD:COD ratio was 0.3, total suspended solids was 1990, ammonia nitrogen was 0.026, nitrate nitrogen was 8.515, phosphate was 5.579, total dissolved soilds was 1420.6, and lead concentration was 0.0210 and cadmium 0.0112, chromium 0.0410 is of great significance. From 20 samples, six isolates were able to grow in the wastewater agar media. These isolates were identified molecularly using partial sequences of the 16S ribosomal RNA gene as: Escherichia coli strain DAQSE1, Escherichia coli strain DAQSE2, Staphylococcus aureus strain DAQSE3, Pseudomonas aeruginosa strain DAQSE4, Enterobacter cloacae strain DAQSE5, Escherichia coli strain DAQSE6. A batch experiment was conducted to investigate bioremediation ability of some heavy metals by isolated bacterial strains. The results showed removal efficiency for lead ranged from 47% to 100%, and the most efficient strain was Staphylococcus aureus DAQSE3 with 100% removal efficiency. The cadmium removal efficiency ranged from 80% to 100%, with the most efficient Enterobacter cloacae DAQSE5 and Staphylococcus aureus DAQSE3 with 100% removal efficiency. The chromium removal efficiency ranged from 73% to 100%, with the most efficient Enterobacter cloacae DAQSE5 and Staphylococcus aureus DAQSE3 with 100% removal efficiency.
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1. Akdemir, T. (2024). Concentrations of trace elements in the effluent of municipal wastewater treatment facilities along the Turkish beaches and evaluation of associated human health risks. Frontiers in Marine Science, Frontiers in Marine Science, 11,1521449.
2. Agoro, M.A., Okoh, O.O., Adefisoye, M.A., &Okoh, A.I. (2018). Physicochemical Characteristics of Wastewater in Three Representative South African Locations. Sewage Treatment Facilities, Pol. Journal. Environmental Studient, 27(2), 491-499.
3.Auied, H.G., Dhahir, S.A., & Sultan, M.A. (2025). Assess the Concentrations of Various Heavy Metals in Industrial Effluent at Al-Dora Oil Refinery, Journal of Engineering, 31(2).
4. Sreedevi, P.R., Suresh, K., & Jiang, G. (2022). An Overview of Procedures and Uses for Bacterial Bioremediation of Heavy Metals in Wastewater, Journal of Water Process Engineering, 48, 102884.
5. Agoun, N.A., & Avci, F.G. (2024). Phycoremediation: A Method for the Bioremediation of Heavy Metals from Wastewater, Journal of Chemical Technology & Biotechnology, 100(1), 13-23.
6. Goswami, R.K., Agrawal, K., Shan, M.P., &Verma, P. (2022). Bioremediation of heavy metals from wastewater: a current perspective on microalgae‐based future Get access Arrow, Applied Microbiology International, 4(1),701-717 .
7. Wang, N., Qiu, Y., Kaimei, H., Huang, C., Xiang, J., Li, H., Tang, J., Wang, J., & Xiao, T. (2021). Influence of plant species and xanthation functions in the one-step production of cake-like biosorbents from plant biomass for the effective extraction and recuperation of heavy metals. ,Chemosphere, 266, 129129.
8. Zaynab, M., Al-Yahyai, R.A., Ameen, Y., Sharif, L., Ali, M., Fatima, K. A., & Khan, S. Li (2022). Health and environmental effects of heavy metals. Journal of King Saud University, 34(1), 101653.
9. Zhang, L., Tan, X., Chen, H., Liu, Y., & Cui, Z. (2022). Effects of agriculture and animal husbandry on heavy metal contamination in the aquatic environment and human health in huangshui river basin, Water, 14(4), 549.
10. Abid-Al-khaim, N.J., & Abbas, A.F. (2020). Characterization of Heavy Metal-Resistant Pseudomonas aeruginosa Isolated from Wastewater of a Battery Manufacturing Facility in Baghdad for Bioremediation , Biochem Cell Arch, 20(2), 6397-6405.
11. lgiri, B.E., .Okoduwa, S.I.R., Idoko, G.O., Adeyi, A.O., & Ejiogu, I.K. (2018). A review of toxicity and bioremediation of heavy metal-contaminated ecosystems from tannery wastewater, Journal of Toxicology,1, 2568038.
12. Abd-Alkhadim, N.J., & Abbas, A.F. (2020). Selective Isolation of Heavy Metal-Resistant Bacteria from Wastewater, Al-Qadisiyah Journal of Pure Science,25(4), 1-12.
13. Loy, A., et al. (2002).Applied and environmental microbiology 68, 5064-5081.
14. Sambrook, J., & Russell., D.W. (2006). Cold Spring Harbor Protocols.pdb-prot4455.
15. Sonune, N., & Garode, A. (2018). Isolation, characterisation, and identification of the extracellular enzyme-producing Bacillus licheniformis from urban wastewater, together with an assessment of its biodegradability,Biotechnology Research and Innovation.
16. Sun, Y., Chen, z., Guangxue., W., Qianyuan, W., Zhang, F., Zhangbin, N. (2016). Characteristics of water quality of municipal wastewater treatment. plants in China: Implications for resources utilization and management.
17.Sohail, B., Baker, M.A., & Hussain, M.I. (2012). Pakistani Municipal Wastewater Treatment for Irrigation.
18.Al-Qasir, M.K.K (2012). Environmental Impact Assessment of Wastewater Treatment Project Outflow on Iraq's Diwaniyah River Water Quality.
19. Etsuyankpa, M., Augustine, A., Musa, S., Mathew, J., Ismail, H., Salihu, A., & Mamman, A. (2024). A Comprehensive Review of Wastewater Characteristics, Treatment, and Disposal, Journal Application Science Environmental . Management, 28(5), 1553-1572.
20. Odjadjare, E.E.O., & Okoh, A.I. (2010). Urban Municipal Wastewater Effluent's Physicochemical Quality and Its Effect on the Receiving Environment, Springer Science, 170,383-394.
21. Agoro, M.A., Okoh, O.O., Adefisoye, M.A., & Okoh, A.I. (2018). Physicochemical Characteristics of Wastewater at Three Representative South African Sewage Treatment Facilities, Pol Journal Environmental Studient, 27(2), 491-499.
22. Bashan, L.E.D., & Bashan, Y. (2004). Recent Developments in Phosphorus Removal from Wastewater and Its Future Application as Fertilize (1997–2003), Water Research, 38(19), 4222-4246.
23.Drecht, G.V., Bouwman, A.F., Harrison,J., & Knoop, M.(2009). Global Urban Wastewater Nitrogen and Phosphate Levels, Advancing Earth and Space Sciences, 23(4),1970–2050.
24. Dubber, D., & Gray, N.F. (2010). Replacement of chemical oxygen demand (COD) with total organic carbon (TOC) for monitoring wastewater treatment performance to minimize disposal of toxic analytical waste. Journal of Environmental Science and Health, 45(12), 1595–1600.
25. Bader, A.C., Hussein, H.J., & Jabar, M.T. (2022). COD Ratio: Wastewater and Industrial Water Pollution Indicator, International Journal of Soecial Education, 37(3).
26. Kulkarni, S.J. (2015). Wastewater Treatment for Lead Removal: A Review, Ijsrset, 1(1), 2395-1900.
27.Genchi, G., Sinicropi, M.S., Lauria, G., Carocci, A., & Catalano, A. (2020). The effects of cadmium Toxicity, International Journal Environmental Research, 17(11), 3782.
28. Jabari, M., Aqra, F., Shahin, S., & Khatib, A. (2009). Monitoring chromium content in tannery wastewater, Journal of the Agrentine Chemical Society, 97(2), 77-87.
29. Huang, M., Pan, J., & Zheng, L.P. (2015). Removal of heavy metals from aqueous solutions using bacteria, Journal of Shanghai University, 5, 253-259.
30.Sharma, L., Sharma, P., Chauhan, N., & Abideen, Z. (2022). Bacterial isolate bioremediation of wastewater, Journal of Pharmaceutical Negetive Results, 13,10.
29. Jakovljevic, V., Grujic, S., Simic, Z., Ostojic, A., & Radojevic, I . (2022). Identifying the optimal mix of indigenous microorganisms exhibiting the highest biosorption capacity for the removal of heavy metals from wastewater, Frontiers Microbiol, 13.
30. Khan,Z., Nisar,M.A., Zajif, S., Arshad, H.M.N., & Rehman., A. (2015). Cadmium resistance mechanism in Escherichia coli P4 and its potential use to bioremediate environmental cadmium, Applied Microbiology and Biotechnology, 99, 10745-10757.
31.Shakoori, F.R., Tabassum, S., Rehman, A., & Shakoori, A.R. (2010). Isolation and Characterization of Cr6+ Reducing Bacteria and their potential use in bioremediation of Chromium-Contaminating Wastewater Bioremediation, Pakistan Journal zoology, 42(6), 651-658.
32. Baskaran, L., Chidambaram, A.L., Mahakavi, T., & Santhoshkumar, M. (2014). Bioremediation of chromium by Bacillus subtilis and Pseudomonas aeruginosa, International of Journal of Current Microbiology and Applied Sciences, 3(9), 715-719.
33.Sethuraman, P., & Balasubramanian, N. (2010). Removal of Cr(VI) Bacillus subtilis, Pseudomonas aeruginosa, and Enterobacter cloacae were used to extract the bacteria from an aqueous solution, 2(6), 1811-1825.
34. Abskharon, R.N.N., Rab, E. S.M.F.G., Hassan, S.H.A., & Shoreit, A.A.M. (2009). Reduction of Toxic Hexavalent Chromium by E. coli, Global Journal of Biotechnology & Biochemistry, 4(2), 98-103.
35. Pattnaik, S., Dash, D., Mohapatra, S., Pattnaik, M., Marandi, A.K., Das, S., & Samantaray, D.P. (2020). Improvement of rice plant productivity by native Cr(VI) reducing and plant growth promoting soil bacteria Enterobacter cloacae, Chemosophere, 240, 124895.
36. Owojori, G.O., Lateef, S.A., & Ana, G.R.E.E. (2024). Hospital Source Wastewater Treatment Plant's Pathogenic Bacteria, Nutrients, and Antibiotic-Resistant Bacteria Elimination, Environmental Science and Pollution Research, 31, 10785-10801.
37.Hammadi, A.H. (2015). Isolation and identification of harmful microorganisms from the Al-Rustamiyah station and their implications for public health, Iraqi Journal of Science, 56(3) 2481-2487.
38. Abbas, A.F., & Muhammed, A.M. (2023). Molecular Characterization of Certain Lead-Resistant Bacteria Isolated from Contaminated Environments, AIP Conference Proceedings, 2834, 020018.
39. Nyika, J., & Dinka, M.O. (2022). A mini-review on wastewater treatment through bioremediation towards enhanced field applications of the technology, AIMS Environmental Science, 9(4), 403-431.
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