The effect of Beta - aminobutyric acid on Malondialdehyde and urea levels in type 2 diabetic male rats
DOI:
https://doi.org/10.36320/ajb/v17.i3.20101Keywords:
Diabetes mellitus, MDA, Urea, Rats, ELISAAbstract
Diabetes mellitus, a complex metabolic disorder characterized by hyperglycemia. Objective: This study aimed to evaluate the protective effect of beta-aminobutyric acid (BABA) on streptozotocin (STZ)-induced diabetes in male rats, by studying its effect on Malondialdehyde (MDA), and urea. Thirty adult male albino rats models of diabetes mellitus were randomly assigned to six groups of five pets each, age of (8-10 weeks). All rats were in good health, with weights ranging from 140 to 160 grams, Positive control groups treated with normal saline, Negative control groups treated only with streptozotocin, Group A treated with 50 mg/kg of STZ then BABA, Group B treated with 100 mg/kg strptozotocin then BABA, Group C treated with 150 mg/kg of strptozotocin then BABA, and Group D treated with 200 mg/kg of strptozotocin then BABA. Enzyme-linked immunosorbent assay (ELISA) was utilized to evaluate MDA, and urea levels in serum. The results of the study show MDA levels elevated in the rats studied (Control -,A, B, C, and D) which recorded (4.47 ±1.93 mg/dl), (3.01 ±0.76 mg/dl), (3.07 ±0.41 mg/dl), and (2.04 ±0.22 mg/dl), compared control group(4.61 ±0.15 mg/dl), results indicate high levels of urea in the groups of rats studied (Control -,A, B, C, and D) which recorded (115.33± 39.01) mg/dl, (107.67±22.25)mg/dl, (70.67±22.06)mg/dl, (73.00±13.65)mg/dl, and (57.67±3.71) mg/dl, compared to control+ (44.33±3.48)mg/dl. The results indicated correlation coefficient between weight and sugar Level is 0.027. This indicates a very weak positive correlation between the two variables, that there is no significant linear relationship between weight and sugar level in rats studied. BABA plays an important protective role as an antioxidant against STZ-induced oxidative stress.
Downloads
References
1. Ghasemi, A., & Jeddi, S. (2023). Streptozotocin as a tool for induction of rat models of diabetes: a practical guide. EXCLI journal, 22, 274–294. https://doi.org/10.17179/excli2022-5720.
2. Amrolahi, Z., Avandi, S. M. and Khaledi, N. (2022). The effect of six weeks’ progressive resistance training on hippocampus BDNF gene expression and serum changes of TNF-α in diabetic wistar rats. Journal of Sport and Exercise Physiology, 15(1), 1-10. doi: 10.52547/joeppa.15.1.1
3. Lertpatipanpong, P., Lee, J., Kim, I., Eling, T., Oh, S. Y., Seong, J. K., & Baek, S. J. (2021). The anti-diabetic effects of NAG-1/GDF15 on HFD/STZ-induced mice. Scientific reports, 11(1), 15027. https://doi.org/10.1038/s41598-021-94581-y.
4. Furman B. L. (2021). Streptozotocin-Induced Diabetic Models in Mice and Rats. Current protocols, 1(4), e78. https://doi.org/10.1002/cpz1.78
5. Rahmati, M., Taherabadi, S.J. (2021). The effects of exercise training on Kinesin and GAP-43 expression in skeletal muscle fibers of STZ-induced diabetic rats. Sci Rep 11, 9535. https://doi.org/10.1038/s41598-021-89106-6.
6. Morales, M., & Munné-Bosch, S. (2019). Malondialdehyde: Facts and Artifacts. Plant physiology, 180(3), 1246–1250. https://doi.org/10.1104/pp.19.00405.
7. Cordiano, R., Di Gioacchino, M., Mangifesta, R., Panzera, C., Gangemi, S., & Minciullo, P. L. (2023). Malondialdehyde as a Potential Oxidative Stress Marker for Allergy-Oriented Diseases: An Update. Molecules (Basel, Switzerland), 28(16), 5979. https://doi.org/10.3390/molecules28165979.
8. Gao X, Zhang S, Wang P, Jaroniec M, Zheng Y, Qiao S-Z. (2024) Urea catalytic oxidation for energy and environmental applications. Chemical Society Reviews. 53(3):1552-91. https://doi.org/10.1039/D3CS00963G.
9. Yu Y, Lu Q, Chen F, Wang S, Niu C, Liao J, et al. Serum untargeted metabolomics analysis of the mechanisms of evodiamine on type 2 diabetes mellitus model rats. Food & Function. 2022;13(12):6623-35. https://doi.org/10.1039/D1FO04396J.
10. Al-Dulaimy EA, Jasim MA. (2022). Effect of non-protein amino acids β-amino butyric acid (BABA) on the intestinal G-bacterial community.
DOI: 10.47750/pnr.2022.13.S02.31.
11. Thamer, IAH. Jasim, MA. (2021) Anti-diabetic effect of β-amino Butyric Acid in Streptozotocin induced Rats. Systematic Reviews in Pharmacy, 12 (2), 139-144. doi:10.31838/srp.2021.2.14.
12. Saada H, Eltahawy N, Hammad A, Morcos N. (2016). Gamma amino butyric acid attenuates liver and kidney damage associated with insulin alteration in γ-irradiated and streptozotocin-treated rats. Arab Journal of Nuclear Science and Applications.; 49:138-50.
13. Cary N. (2012). Statistical analysis system, User's guide. Statistical. Version 9. SAS Inst Inc USA.
14. Aktas I, Gur FM. (2021). The effects of thymoquinone and β-aminoisobutyric acid on brain tissue of streptozotocin-induced diabetic rats. International Journal of Veterinary and Animal Research (IJVAR). 4(1):01-6.
15. Aktaş, İ., & Mehmet Gür, F. (2022). Hepato-protective effects of thymoquinone and beta-aminoisobutyric acid in streptozocin induced diabetic rats. Biotechnic & histochemistry: official publication of the Biological Stain Commission, 97(1), 67–76. https://doi.org/10.1080/10520295.2021.1949041.
16. Eltahawy, N. A., Saada, H. N., & Hammad, A. S. (2017). Gamma Amino Butyric Acid Attenuates Brain Oxidative Damage Associated with Insulin Alteration in Streptozotocin-Treated Rats. Indian journal of clinical biochemistry: IJCB, 32(2), 207–213. https://doi.org/10.1007/s12291-016-0597-2.
17. Zhu, Y., Devi, S., Kumar, M., Dahiya, R. S., & Tarkeshwer (2021). Evaluation of Gamma Amino Butyric Acid (GABA) and Glibenclamide Combination Therapy in Streptozotocin Induced Diabetes. Endocrine, metabolic & immune disorders drug targets, 21(11), 2005–2016. https://doi.org/10.2174/1871530320666201208110945.
18. Gur, F. M., & Aktas, I. (2021). The ameliorative effects of thymoquinone and beta-aminoisobutyric acid on streptozotocin-induced diabetic cardiomyopathy. Tissue & cell, 71, 101582. https://doi.org/10.1016/j.tice.2021.101582.
19. Luka C, Istifanus G, Mayel M. (2020). Determination of the antidiabetic property of the aqueous extract of biophytum sensitivum on streptozotocin induced diabetic rats. GSJ. 8(8): 2137-2150.
20. Voss, C., Brachmann, K., & Hartmann, K. (1988). Effect of streptozotocin on transaminases, creatinine and urea in serum of rats. Experimental and clinical endocrinology, 92(1), 37–42. https://doi.org/10.1055/s-0029-1210779.
Downloads
Published
Issue
Section
License
Copyright (c) 2025 Shams Husham Al-Naqqash, Dr. Mohammed Abbas Jasim
This work is licensed under a Creative Commons Attribution 4.0 International License.
which allows users to copy, create extracts, abstracts, and new works from the Article, alter and revise the Article, and make commercial use of the Article (including reuse and/or resale of the Article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made and the licensor is not represented as endorsing the use made of the work.
