Evaluation of antibiotic resistance and virulence genes of Listeria monocytogenes isolated from aborted women aborted in Hillah city
DOI:
https://doi.org/10.36320/ajb/v17.i3.20376Keywords:
virulence factor genes, PCR, antibiotic susceptibility, Listeria monocytogenesAbstract
Pregnant women who contract Listeria monocytogenes may experience invasive disease, miscarriage, stillbirth, and severe infections in the developing fetus. This study sought to identify antibiotic susceptibility patterns, Listeria monocytogenes in aborted women, and the genes that encode its virulence factors.
Methods: In total, 100 specimens were obtained from multiple hospitals in Hilla city between August 2024 and December 2024. Of these, 70 high vaginal swabs were taken from pregnant women with a history of abortion and 17 placental tissue specimens (i.e., pieces of the placenta) were taken from aborted women. Using the conventional disk diffusion method, antibiotic susceptibility to a panel of ten antibiotics was ascertained. actA, inlA, and prfA genes were also checked for by PCR in order to determine the presence of these virulence factors.
Results: There was a total of 23 isolates of Listeria monocytogenes found (14 placenta and 9 vaginal swabs). antibiotic susceptibility teste for streptomycin (96.65%; n = 22), erythromycin (86.95%; n = 20), gentamicin (73.91%; n = 17), and tetracycline (60.86%; n = 14), high rates of resistance were noted. The actA (100 %; n = 23), inlA (86.95 %; n = 20), and prfA (95.65%; n = 22) are the distribution of the L. monocytogenes virulence genes.
Conclusion: Control measures include raising public awareness of listeriosis infections, employing quick diagnostic techniques for efficient treatment, conducting susceptibility testing, choosing the appropriate antibiotics, and preventing the bacteria's effects on expectant mothers.
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