Production of bacteriocin from local isolate Lactobacillus acidophilusand its nanoscale improvement and study of its properties
DOI:
https://doi.org/10.36320/ajb/v14.i3.22861Keywords:
bacteriocin - chitosan - nanotechnology - ionic gelatinization - Lactobacillus acidophilusAbstract
The study was conducted on the bacteriocin produced by the isolate of
Lactobacillus acidophilus under optimal conditions that were approved according to a
previous study after investigating its ability to inhibit a number of test bacteria that
divided into two types, including positive bacteria, Staphylococcus aureus Bacillus
cereus and negative bacteria Salmonella typhimurium, coli. E The diameter of the
damping halo ranged between 9, 8 and 6, 6, respectively .Then the bacteriocin was
partially purified by ammonium sulfate with a saturation average of about 70%, and
then dialysis using Spectra Dialyzer tubing Cat. No 3787 for 18 hours to get rid of
salts and impurities. The obtained bacteriocin was measured so that the kidneys
reached about 34500 units, and the total effectiveness was about 115000 units/mg. To
improve traits of bacteriocin, it was coated with a chitosan biopolymer, which also
has an enhanced role in eliminating microorganisms that cause food spoilage and
spoilage through the ionic gelatinization method and to obtain particles with
nanoscale dimensions. /ml with a fixed percentage of chitosan has reached about 0.2
g. The reason behind the success of the encapsulation process was due to the ability of
the inner cavity of the laboratory-created nano chitosan to accommodate the studied
amount of bacteriocin. Nano-characterization of the nanoparticles obtained from
bacteriocin coated with chitosan was coundected by scanning electron microscope
(SEM). It took the form of smooth, dense and uniform clumps of uniform consistency,
compared to the usual bacteriocin, which was in the form of three-dimensional
crystals with a monoclinic system, taking the shape of an almost prism. As for the
analysis of the chemical composition of the synthetic nanoparticles, they were
identified by FTIR spectroscopy at a wavelength of 3435 cm-1, which showed the
presence of all the molecules that make up bacteriocin without any change in their
arrangement inside the chitosan cavity. The same is true for testing the samples using
the X-ray diffraction device, which showed that the match was completely and
correctly through the chemical phases of the standard sample with the synthetic
material, with the compound not losing its structural integrity during the ion
gelatinization process.
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