investigation Resistance and virulence of E. coli in local pathogenic samples
DOI:
https://doi.org/10.36320/ajb/v18.i2.23521Keywords:
E. Coli, OmpT, blaTEM, Pap genesAbstract
Abstract
Background: Escherichia coli is a common opportunistic pathogen responsible for a wide range of infections. The emergence of multidrug-resistant (MDR) and extended-spectrum β-lactamase (ESBL)-producing strains poses a major challenge to treatment.Objective: This study aimed to determine the antibiotic resistance patterns, motility, ESBL production, and distribution of virulence and resistance genes (blaTEM, Pap, and ompT) among E. coli isolates from different clinical sources.Methodology: A total of 39 E. coli isolates were recovered from urine, blood, wound, and stool samples. Identification was performed using the Vitek 2 system, Gram staining, and biochemical tests. Antimicrobial susceptibility testing was conducted using standard methods. ESBL production was detected by the double-disk synergy test (DDST), motility was assessed using semi-solid medium, and gene detection was carried out by conventional PCR.Results: Most isolates were obtained from urine (66.67%). High resistance was observed to amoxicillin-clavulanate (97.44%) and ceftoxime (82.05%), whereas low resistance was seen to meropenem (7.6%). ESBL production was confirmed in 79.49% of isolates. The ompT gene was the most prevalent, followed by blaTEM and Pap.Conclusion: The high frequency of MDR and ESBL-producing E. coli underscores the urgent need for continuous surveillance, rational antibiotic use, and molecular monitoring to control resistance spread.
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