Molecular and Phylogenetic Identification of Some Virulence Genes of Klebsiella Spp. Isolated from Ear Infection in dogs.
DOI:
https://doi.org/10.36320/ajb/v18.i2.23787Keywords:
Klebsiella spp., Molecular identification, 16S rRNA, Virulence genes, Phylogenetic analysis.Abstract
Klebsiella species such as (Klebsiellapneumoniae, Klebsiella aerogenes), opportunistic pathogens belong to the family Enterobacteriaceae are gram-negative bacteria, especially in immunocompromised dogs. A total of No. 50 ear swab samples of dogs, samples were cultured on MacConkeyagar, Blood agar, and Chromogenic agar to isolate Klebsiella spp. followed using Molecular method to confirm the bacterial identity, by PCR amplification 16S rRNA gene, phylogentic analysis and detection of some virulence gene (blaCTX-M, magA, and mrkA), and susceptibility test. Bacteriological analysis revealed that Klebsiella spp. was accounting for a 14% in dogs (85.71%K. pneumoniae, Klebsiella aerogenes 14.28%), they were detected in dogs isolates blaCTX-M 1(14.3%), magA3(42.9%) and mrkA1(14.3%). Sequencing and Phylogenetic analysis confirmed that the majority of isolates belonged to Klebsiella Spp. ( K. pneumoniae,Klebsiella aerogenes), Phylogenetic analysis revealed high identity (99–100%) with reference strains deposited in GenBank (ID: PV490247.1, ID: PV490248.1, ID: PV490249.1, ID: PV490250.1, ID: PV490251.1, ID: PV490252.1, ID: PV490253.1). Klebsiella spp. isolates showed complete resistance (100%) to Amoxicillin/Clavulanic acid and Imipenem, with high resistance to both Amikacin and Cefotaxime (71.4%), while showing complete sensitivity to Levofloxacin, Co-trimoxazole, and Chloramphenicol. In feline isolates, complete resistance (100%) was recorded to Co-trimoxazole, Amoxicillin/Clavulanic acid, and Imipenem, in addition to high resistance to Amikacin(66.6%), while all isolates showed complete sensitivity to Levofloxacin, Chloramphenicol, Trimethoprim, and Cefotaxime. Most of these results were highly significant (P ≤ 0.01).
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