Emergence of macA and macB Genes in Multi-Drug Resistance Serratia Species Isolated from Nosocomial Infections
DOI:
https://doi.org/10.36320/ajb/v18.i2.23908Keywords:
Serratia, macA and macB, Efflux pump, ProteaseAbstract
Serratia species are an opportunistic pathogenic microorganism primarily known as infectious nosocomial agents. 150 diverse specimens were gathered in a sterile manner utilizing appropriately labeled and sterile containers from various regions of Najaf Hospital and private external laboratories. The specimens were then inoculated on culture media, Gram stain, IMVIC tests and catalase tests were done, and the colonies were molecularly characterized by using. Eventually, the isolates were tested against thirteen antibiotics. The results found that all Serratia spp. were Methyl Red (MR), Voges-Proskauer (VP), Indole Production, and Oxidase Test results were all negative. In contrast, all examined isolates had positive findings for both catalase and citrate utilization. The results found that 21 isolates were isolated and classified as Serratia spp. Also, the antibiotic susceptibility of Serratia showed that all tested isolates were susceptible to all tested antibiotics. These isolates showed high resistance to 100% Ampicillin, Cefuroxime, Ceftazidime, cephalexine, and sensitive for antibiotics Imipenim, Chloramphenicol, Levofloxacin, Ciprofloxacin and Aztreonam of the isolates displayed multidrug resistance. The results of protease enzyme phenotypically detection test showed that 52.3% of Serratia isolates were positive to protease production and gave clear zone around the colonies. The most prevalent genes found in Serratia spp. were macA and macB in 100% all isolates for Efflux pump, and it is the main cause of clinical intrinsic and acquired multidrug resistance in bacteria.
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