Gene profile of acrA and acrB efflux pump genes in multidrug-resistant clinical E. coli isolates
DOI:
https://doi.org/10.36320/ajb/v18.i2.24099Keywords:
Escherichia coli; Multidrug resistance; Efflux pumps; acrA and acrB genes; Clinical isolatesAbstract
Background & Objective: Antibiotic resistance in Escherichia coli is a major global challenge. This study aimed to evaluate the prevalence of two key genes, acrA and acrB, and investigate their association with antibiotic resistance patterns in various bacterial isolates.
Methods: A total of 71 E. coli isolates were collected and subjected to antibiotic susceptibility testing. Subsequently, PCR (Polymerase Chain Reaction) was performed to detect the presence of the acrA and acrB genes. Statistical analysis was then conducted to determine the correlation between these genes and the observed resistance phenotypes.
Results: The statistical analysis revealed a significant correlation between the acrA gene and three specific antibiotics (Aztreonam, Azithromycin, and Amikacin). Regarding the acrB gene, a significant statistical association was found with only two antibiotics: Colistin and Aztreonam. No significant correlation was observed with the remaining tested antibiotics.
Conclusion: The findings conclude that the AcrAB efflux pump system is directly involved in mediating resistance to Colistin, Aztreonam, Amikacin, and Azithromycin. Resistance to other antibiotic classes is likely attributed to alternative bacterial defense mechanisms, such as the production of beta-lactamases or alterations in membrane permeability (Porins).
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