DETECTION OF MUTATIONS IN GYRA GENE AMONG CIPROFLOXACIN RESISTANT ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES IN BAGHDAD CITY
DOI:
https://doi.org/10.36320/ajb/v18.i1.24248Keywords:
Ciprofloxacin Resistance, Escherichia coli, Baghdad City, Iraq.Abstract
Quinolone resistant Escherichia coli (QREC) isolation rate was elevated due to the prolonged use of this drug in clinical diseases. Various mechanisms play role in developing the quinolone resistance, mutations in DNA gyrase and topoisomerase II are the most common among them. The aim of this study was to identify the mutations in gyrA that correlated with ciprofloxacin resistance among Iraqi E. coli isolates. Out of 600 clinical specimens (Urine, Blood and infected wounds), 70 (11.67%) isolates were identified as E. coli during 2015 -2016. The higher isolation was recorded from infected wound swabs (23.33%), while the prevalence rate of E. coli isolates from urine and blood samples was (12.27% and 4.49%, respectively). The resistance rate of ciprofloxacin for E. coli was recorded (52.86%) with minimum inhibitory concentration (MIC) ≥ 4μg/ml. Non-synonymous mutations, Ser-83 were detected in quinolone resistant determining region (QRDR) of gyrA in all sequenced QREC isolates (Eco U3, Eco U16 and Eco U58) and other mutations (Val-68-Ile, Ser-72-Pro, Arg-73-Cys and Lys-96-Glu) were also detected. Mutation outside this region was also displayed in Eco U16 isolate, at position (Ala-119) near to the active site of gyrA (Tyr-122).
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