MOLECULAR IDENTIFICATION , PHYTOCHEMICAL CONSTITUENT DETECTION AND ANTIOXIDANT ACTIVITY OF TWO THYME ( THYMUS VULGARIS L.)VARIETIES
DOI:
https://doi.org/10.36320/ajb/v18.i2.24712Keywords:
identification and fingerprinting genotypes , phytochemical constituent detectionAbstract
The current study was conducted during (2025-2026) to determine DNA fingerprint using two molecular markers including RAPDs (Random Amplified Polymorphic DNA’s and ISSR (Inter Simple Sequence Repeat) ., preliminary phytochemical constituent detection and antioxidant of two Thymus vulgaris L. (Thyme ) varieties (Syrian and Iranian ) ethanolic extract . Using RAPD primers , all primers success in distinguishing between two examined Thyme varieties ( Iranian and Syrian ) by giving them a unique fingerprint .Using ISSR markers , all primers failed to give a unique finerprint to Thyme varieties.Primers . In comparison between two markers .RAPD proceeds ISSR marker in most studied criteria. Among examined phytochemical ,Iranian thyme missed Trpenoid , saponin and Fuocoumarins ,while Syrian Thyme missed resin, saponin and phenolate .Antioxidant activity that two plant extract possess antioxidant activity compared to control treatment. , there is increase in extract scavenging activity with increasing concentration compared to control.
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References
1.Al-Rawi A., and Chakravarty, H.L(1988). "Medicinal Plants of Iraq",2th ed. Baghdad
2.Muttalib , L. Y. and Naqishbandi , A. M.(2012). Antibacterial and Phytochemical Study of Iraqi Salvia officinalis Leave Extracts , Iraqi J Pharm Sci, 21(1) :93-97.
3.Abdelaty, N. A. ; Attia , E. Z.; Hamed, A.N. E. and Desoukey, S. Y..(2021). A review on various classes of secondary metabolites and biological activities of Lamiaceae (Labiatae) (2002-2018), J. Adv. Biomed. & Pharm. Sci. 4 , 16-31.
4.Halat, D.H. ; Krayem, M. ; Khaled , S. and Younes , S..(2022). A Focused Insight into Thyme: Biological, Chemical, and Therapeutic Properties of an Indigenous Mediterranean Herb, Nutrients 2022, 14, 2104
5.Owusu, E.; Ahorlu, M. M.; Afutu, E.; Akumwena, A. and Asare, G.A. (2021) . Antimicrobial Activity of Selected Medicinal Plants from a Sub-Saharan African Country against Bacterial Pathogens from Post-OperativeWound Infections, Medical Sciences, 9(2): 23.
6.Trevizan, J. ; Soto , E.; Parra, F. ; Bustos , L. and Parra, C .(2020). Antioxidant activity of nine medicinal plants with commercial potential, IDESIA (Chile), 38( 3): 53-58
7.Ganie SH, UpadhyayP, Das S and Sharma MP (2015) Authentication of Me-dicinal Plants by DNA Markers.Plant Gene, 4, 83-99.
8.Moraes, D.F.C.; Still, D.W.; Lum, M.R. and Hirsch, A.M. (2015) .DNA-Based Au-thentication of Botanicals and Plant-Derived Dietary Supplements: Where Have WeBeen and Where Are We Going?Planta Medica, 81, 687-695.
9.Ahmad, W. ; Muhammad, K. ; Hussain, A. ; Ahmad , H. ; Kahn, K. ; Qarshi , I.A.; Shinwari , K.I. ; Nadeem, M. S. ; Que, Y. ; Khan , A. and Iqbal, J.(2017). DNA Fingerprinting of Essential Commercialized Medicinal Plants from Pakistan American Journal of Plant Sciences , 8, 2119-2132
10.Chung, K.F.; Kuo, W.H.; Hsu, Y.H.; Li, Y.H.; Rubite, R.R. and Xu, W.B. (2017) Molecular Recircumscription of Broussonetia (Moraceae) and the Identity and Taxo-nomic Status of B.Kaempferi var.australis.Botanical Studies, 58, 11.
11.Gorji, A. M.; Poczai, P.; Polgar, Z. and Taller, J. (2011). Efficiency of arbitrarily amplified dominant markers (SCoT, ISSR and RAPD) for diagnostic fingerprinting in tetraploid potato. Am Potato, 88: 226–237.
12.AL-Haidari, H.A.M. (2023). DNA Fingerprinting and GC-MAS analysis of mutation generated by chemical and physical mutagens. Msc thesis, University of Kufa, College of Science, Biology department .pp140
13.AL-Rehbawy,S.M.J.(2022).Molecular variation in Coriander sativum L. in response to gamma ray exposure and their biological activities ,PhD thesis .university of kufa .college of science .pp 162
14.AL-Musawi, M.D. K.(2022). Molecular variation in Catharanthus roseus var. albus G. Don induced by mutagens. Msc thesis, University of Kufa, College of Science, Biology department .pp121
15.Sambrook, J. and Russell, D. W. (2001). In vitro application of DNA by the polymerase chain Reaction, in molecular cloning. A laboratory manual. 3rd ed., Cold Spring Harbor Laboratory Press, New York. Chapter 8: 691-733.
16.Hunter, P. R. and Gaston, M. A. (1988). Numerical index of discriminatory ability of simpson's index of diversity. J. Clin. Mic., 26:2465-2466.
17.Graham, J. and McNicol, R. J. (1995). An examination of the ability of RAPD markers to determine the relationships within and between Rubus spp. Theo. Appl. Gene., 90: 1128-1132.
18.Hussein, A. A. M.(2025).Molecular Marker–Based Diversity Analysis, Phytochemical Profiling and Bioactivity Potency of Two Medicinal Plants. Msc thesis, University of Kufa, College of Science, Biology Department. pp142
19.Ishimaru, K. ; Nishikawa, K. ; Omoto, T. ; Asai, I.; Yoshihira, K. and Shimomura, K. (1995) .“Two flavone 2-glucosides from Scutellaria baicalensis ”. Phytochemistry, 40 (1) : 279–281.
20.Ibrahim, M.M.; Aboud , K.A. and Al-Ansary, A.M.F.(2013). Genetic Variability Among Three Sweet Basil (Ocimum basilicum L.) .Varieties as Revealed by Morphological Traits and RAPD Markers, World Applied Sciences Journal, 24 (11): 1411-1419
21.Vishwanath, K.; Prasanna, K. P. R., Pallvi, H. M.; Rajendra P.; Ramegowda, S. and Devaraju, P. J. (2010). Identification of Tomato (Lycopersicon esculentum) Varieties through Total Soluble Seed Proteins Research Journal of Agricultural Sciences, 2(1):8-12.
22.Shasany, A. K.; Aruna, V.; Darokar ,M. P.; Kalra, A.;Bhal, J .R.; Bansal,R. P and Khanuja, S. P. S. (2002) .RAPD marking of three Pelargonium graveolens genotype with chemotypic difference in oil quality,J Med Arom Plant Sci, 24 ,733-737
23.Fico ,G.; Sapda, A.; Brach, A., Agradic, E.; Morillib , I. and Tomea, F .(2003). RAPD analysis and flavonoid composition of Aconitum as an aid for taxonomic discrimination, Biochem Syst Ecol, 31 , 293-301
24.Stefan, F.M. ; Berindean , I.; Tican, A. and Racz, I.(2024). Using rapd markers to establish dna fingerprint and to study the genetic variability discrimination between two romanian potato varieties, Scientific Papers. Series A. Agronomy, Vol60( 2) :616-623.
25.Dalir, M. and Safarnejad, A .(2016). Morphological, Molecular and phytochemical Variation in Some Thyme Genotypes, Bulletin of Environment, Pharmacology and Life Sciences , 5 (11): 25-35
26.Chen ,S.; Dai,T.; Chang, Y.; Wang, S.; Ou, S.; Chuang, W.; Cheng, C.; Lin, Y.; Lin, L. and Ku, H.(2013). Genetic diversity among Ocimum species based on ISSR, RAPD and SRAP markers, AJCS 7(10):1463-1471 .
27.AL-Shujairi , Z.H.H.(2023). Molecular, chemical characterization and biological activity of some Ocimum basilicum L. cultivars. Msc thesis ,University of Kufa ,College of Science .pp 117
28. AL-Tamimi, A. J. T. (2020 ). Genetic variation among Zea mays genotypes using start codon targeted (SCoT) markers polymorphism. SABRAO Journal of Breeding and Genetics ,52 (1) 1-16.
29.Hromadova , Z.; Galova , Z. ; Mikolasova , L.; Balazova, Z. , Vivodík, M.and Chnapek, M.(2023). Efficiency of RAPD and SCoT Markers in the Genetic Diversity Assessment of the Common Bean, Plants 2023, 12, 2763
30.Nugraha , D. ; Yusuf , A.L.; Wahlanto, P.(2023). Narrative Review: Optimation of Ethanol as a Solvent for Flavonoid Compounds in Papaya Leaf Extraction , Ad-Dawaa Journal Of Pharmacy
31.Dhawan, D. and Gupta J. (2017) Comparison of Different Solvents for Phytochemical Extraction Potential from Datura metel Plant Leaves , Int. J. Biol. Chem., 11 (1): 17-22,
32.Abdelbakya, A.S. and Diab, Y.M.(2021). Effect of Various Extraction Methods and Solvent Types On Yield, Phenolic and Flavonoid Content and Antioxidant Activity of Spathodea nilotica Leaves , Egypt. J. Chem , 64(12): 7583 – 7589.
33.Kebede, M. and Admassu , S.(2019). Application of Antioxidants in Food Processing Industry:Options to Improve the Extraction Yields and Market Value of Natural Products , Adv Food Technol Nutr Sci Open J. ,5(2): 38-49
34.Boufadi, M.Y.; Keddari ,S.;Moulai-Hacene, F. and Chaa S. (2021). Chemical Composition, Antioxidant and Anti-Inflammatory Properties of Salvia Officinalis Extract from Algeria , Pharmacog J. 13(2): 506-15.
35.Hamad ,A. M .A .(2021). Determination antioxidant activity of Green tea ( Camellia sinensis),Thyme (Thymus vulgaris L) and Salvia officinalis (Sage), South Asian Research Journal of Pharmaceutical Sciences, 3 (4 ):52-55
36.Aslan ,K. ; Koparb , E.E.; Kelleb, K.; Karageçilic , H.; Yilmazd , M. A. ; Cakird, O. ; Alwasele, S. and Gulcina, I. (2025). Phytochemical profile and bioactive properties of sage (Salvia fruticosa) and thyme (Thymus vulgaris) extracts International journal of food properties , 28(1 ):1-15.
37.Hussein , M.E.A.; Labib , S-E.M.; Awad , A.E. and Abo El-Maati, M.F.(2024). Antioxidant and antitumor activity of Thyme leaves water extract . Zagazig J. Agric. Res., 51 (5) : 1057-1067.
38.Karoui, I. J.; Msaada, K.; Abderrabba , M. and Marzouk, B..(2016). .Bioactive Compounds and Antioxidant Activities of Thyme-Enriched Refined Corn Oil , J. Agr. Sci. Tech. , 18: 79-91
39.Roby ,M. H. H. ; A.Sarhan, M. ; Selim, K. A. and Khalel, K.I..(2013). Evaluation of antioxidant activity, total phenols and phenolic compounds in thyme (Thymus vulgaris L.), sage (Salvia officinalis L.), and marjoram (Origanum majorana L.) extracts Industrial Crops and Products 43 , 827–831
40.Nwozoa , O.S.; Effionga, E. M. ; Ajab , P. M. and Awuch, C. G. (2023). Antioxidant, phytochemical, and therapeutic properties of medicinal plants: a review, International journal of food properties , 26( 1):359–388
41.Stankovic, M.S..(2011). Total phenolic content, flavonoid concentration and antioxidant activity of Marrubium peregrinum L. extracts, Kragujevac J. Sci. 33 (2011) 63-72
42.Jadid ,N.; Hidayati ,D.; Hartanti, S. R.; Arraniry, B. A.; Rachman, R. Y. and Wikanta ,W..(2017). Antioxidant activities of different solvent extracts of Piper retrofractum Vahl. using DPPH assay ,AIP Conf. Proc. 1854, 020019-1–020019-6; doi: 10.1063/1.4985410
43.Behzadi, P.;Behzadi ,E. ;Yazdanbod, H.; Aghapour,Belhachemi, M. H.; Belmir, S. ;Mebarek, M. O.; Reffis, M and Achour, F. Z. (2024). Evaluation of antioxidant activity in crude polysaccharide extracts from two date varieties (Tazerzait “Azerza” and Deglet-Nour), Journal of agriculture and applied biology , 5( 1):75 – 85
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