Exopolysaccharide vaccine extracted from C.freundii induce immunity against infectional pathogens
DOI:
https://doi.org/10.36320/ajb/v9.i2.7586Keywords:
exopolysaccharide, fungicide Bentazole, immunomodulater activity, cytokineAbstract
Eleven isolates of Citrobacter. freundii were isolated from100 stool and urine specimens taken from diarrhea and recurrent –urinary tract infections (UTI) patients attended to AL-Sadar Hospital. specimens were cultured on specific media, then bacterial isolates were identified depending on morphological, biochemical and VITK-2. The results showed that the C. freundii comprise 11 / 78 (14.1 %) of total of positive bacterial growth on macConky agar from which 10.2 % isolated from patients suffering from diarrhea and 3.8 % isolated from UTIs . The results of determine efficient isolate in exopolysaccharide(EXP ) production revealed that C. freundii were isolated from recurrent -UTI was more mucoidy colonies and appeared highly biofilm formation with absorbancy 0.890 . The results of LD50 observed that a live suspension of C. freundii was 1×107 ,while HK E,coli was 2.3×108 finally EXP was 390 mg/ml .The immunomodulatory activity appear that EXP relaease moderate levels of proinflammatory biomarker ( IL-6 , TNF-α and IL-12) that enable immune system to clear pathogen . While HK E.coli and whole bacteria release higher proinflammatory biomarker especially during post-dose that lead to increased the hypersensitivity and hyperresponses of immune system. In other hand EXP stimulate release moderate concentration of anti-inflammatory biomarker IL-10 and higher eleveated during post dose but HK E.coli and whole bacteria higher concentration during primary dose while in post dose lower than EXP vaccine. A significant immunogenicity and effective protection was observed in immunized groups with EXP challenged with 100 of LD50 of a live C. freundii compared with control group of mice which died within first days .
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