Phenotypic and Genotypic Detection of PER β-Lactamase among Carbapenem Resistant Klebsiella pneumoniae in Hilla Hospitals.
DOI:
https://doi.org/10.36320/ajb/v9.i2.7587Keywords:
Klebsiella pneumoniae, extended spectrum beta lactamase, blaPER, PCRAbstract
This study investigates the prevalence of blaPER gene in clinical isolates of Klebsiella pneumoniae. During the period from April to August 2011, a total of 801 various clinical samples were collected from different hospitals in Hilla city. Of these,117 isolates were specified as K.pneumoniae . High prevalence of K.pneumoniae isolates were detected in stool samples 38 (27%) followed by sputum 19 (15%). All 117 K.pneumoniae isolates were primarily screened for- lactams resistance, 91 (78%) were found to be screen positive. β- lactam resistance isolates were underwent antimicrobial susceptibility to 26 antibiotics by Kirby-Bauer disk diffusion methods .High resistance rate was recorded for penicillins (carbenicillin and ampicillin) with rates of resistance of (99%) and (94.5%),respectively .Carbapenem resistance was reported in 17 (18.7%) of K. pneumoniae isolates. Phenotypic detection of extended spectrum β- lactamase by CHROMagar technique identified a proportion of 17(100%) as ESBL producers. The presence of blaPER gene was checked by Polymerase Chain reaction (PCR) and confirmed in 10 (82.3%) of isolates.
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