Molecular Detection and Genotyping of Babesia species in cattle in Baghdad city

Authors

  • Abdelrasol .KH. Saeed College of Medical and Health technology/Middle Technical University/Iraq
  • Mohammed.TH.Salih College of Veterinary Medicine/University of Baghdad/Iraq
  • Mohammed Sh. Jebur College of Medical and Health technology/Middle Technical University/ Iraq

DOI:

https://doi.org/10.36320/ajb/v9.i2.7599

Keywords:

Babesia bovis, PCR, phylogenetic tree, Gene Bank

Abstract

In cross-sectional study carried in areas surrounding  Baghdad province (Dora , Nahrawan, Tajy and Abu Graib )respectively. During June -December 2016, for detection of cattle Babesiosis. One hundred fifty (150) clinically healthy local cattle breeds of different ages, and from both sex were included by molecular detection of Babesiosis by Conventional polymerase chain reaction PCR .  Results revealed that the  species of Babesia during this study were Babesia  bovis  and they were detected in 15/150 of cases ( 10% ), Gene sequencing and phylogenetic tree were done for the isolated species and the results revealed the similarity of our detected species with other species of neighbor countries published in Gene Bank for  Babesia bovis  with little differences .infection detected in all parts of the study and included all age groups of cattle. Our isolates showing clear gene Diversity compared with others genes recorded in neighboring countries.

Downloads

Download data is not yet available.

References

O.I.E. Bovine babesiosis in Terrestrial manual.Vol.1.Ch 2.4. Office international Des Epizooties,World Health Organization for Animal Health,Paris, France.p 1

Bock,R.,Jackson,L.,Devos,A. and Jorgensen,W.(2004).Babesiosis of cattle.Parasitology. 129:247-269.

Goff WL, Johnson WC, Molloy JB, Jorgensen WK, Waldron SJ, Figueroa JV, Matthee O, Adams DS, McGuire TC, Pino I, Mosqueda J. Validation of a competitive enzyme-linked immunosorbent assay for detection of Babesia bigemina antibodies in cattle. Clinical and Vaccine Immunology. 2008 Sep 1;15(9):1316-21.

Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ. Basic local alignment search tool. Journal of molecular biology. 1990 Oct 5;215(3):403-410.

Mtshali MS, Mtshali PS. Molecular diagnosis and phylogenetic analysis of Babesia bigemina and Babesia bovis hemoparasites from cattle in South Africa. BMC veterinary research. 2013 Aug 8;9(1):154

AbouLaila M, Yokoyama N, Igarashi I. Development and evaluation of two nested PCR assays for the detection of Babesia bovis from cattle blood. Veterinary parasitology. 2010 Aug 27;172(1):65-70.

Almeria,S.,Castella,J.,Ferrer,D.,Ortuno,A.,Estrade,-Pena,A.,and Gutierrez,J.F.(2001).Bovine piroplasma in Minorca(Baleric island , Spain): a compartion of PCR-based and light microscopy detection.Vet.parasitol.99:249-259.

Fisher,TG.,McElwain,TF.,Palmer,GH:Molecular basis for variable expression of merozoites surface antigen gp45 among American isolates of Babesia bigemina. Infect immune.2001,69:3782-3790. .

Snedecor GW CWG. Statistical methods .7th ed.Iowa.The state University Press American:1989.

Khawla Hussein Sabbar Al-Thabhawi (2015) Molecular Detection of Babesia bovis and Babesia bigemina in cattle in Al-Qadisiyah Province.Msc thesis .College of Veterinary Medicine/University of Al –Qadisiyah.

Saad F, Khaisroon M, Khan K, ul Akbar N. Prevalence and Molecular Detection of Babesiosis in the Slaughter Animals of Peshawar Khyber Pakhunkhawa Pakistan. Int. J. Curr. Microbiol. App. Sci. 2015;4(8):1030-1036.

Omima Ibrahim, Zaid Taha and Salih Jassim (2012).Prevalence of Babesia bovis in cattle in Tikreet city and its surrounding ,with heamatological study.Tikrit Journal of Pure Science:17(2) 2012.

Muhanguzi, D., Matuvu, E. and Waiswa, C. (2010). Prevalence and Charactarization of Theileria and Babesia species in cattle under Different Husbandry System in Western Uganda .Int.Jou.of Anim.and Vet. Advances.2(2):51-58.

Smeenk, I., Kelly, P. J.,Wary,K., Musuka, G., Trees, A.J. and Jonjegan, F.(2000).Babesia bovis and Babesia bigemina DNA detected in cattle and tick from Zimbabwe by polymerases chain reaction . Journal of the South Africa Veterinary Association. 71(1):21-24.

Mustafa, B.H.S.(2011).Study on some epidemiological factors of hard tick(Ixodidae) in sheep in Sulaimania province with trials to immunize rabbits against larval extracts of Hyalomma anatolicum anatolicum. Ph.D Thesis , Sulaimania University.

Simuunza , M.C. (2009). Differential diagnosis of tick-borne diseases and population genetic analysis of Babesia bovis and Babesia bigemina.Ph.D thesis .faculty of Veterinary Medicine –University of Glasgow.

Downloads

Published

2017-10-22

How to Cite

.KH.Saeed, A., TH.Salih, M., & Sh. Jebur, M. (2017). Molecular Detection and Genotyping of Babesia species in cattle in Baghdad city. Al-Kufa University Journal for Biology, 9(2), 65–73. https://doi.org/10.36320/ajb/v9.i2.7599

Similar Articles

1 2 3 4 5 6 7 > >> 

You may also start an advanced similarity search for this article.