Frequency of Metallo-β-Lactamases Producing Klebsiella pnumoniae in Burn Wound Isolates
DOI:
https://doi.org/10.36320/ajb/v9.i2.7600Keywords:
Metallo-β-Lactamases, Klebsiella pnumoniae , Burn WoundAbstract
From the period 1 /3 /2016 to 30 / 8 / 2016, 210 swabs were collected from the burn patients hospitalized in different hospitals in Baghdad City: Al-Karama Teaching Hospital, Special Burn Hospital, Central Teaching Laboratories, Child protection Teaching Hospital, Imam Ali Hospital. Forty two isolates (37.5 %) showed a growth of Klebsiella pneumonia, and the remaining isolates (62.5%) were belonged to other bacteria. Identification of all isolates were carried out depending on macroscopic, microscopic characterizations, conventional biochemical tests and Api 20E system. Metallo -β lactamase (MBL) enzymes were screen by three different phenotypic methods (DDST, CMDT and MHT) in which 10µg meropenem antibiotic disk has selected to all phenotypic methods.
Susceptibility test done with antibiotic disks of cefotaxime, ceftazidime, aztreonam, imipenem, meropenem, gentamicin, amikacin, ciprofloxacin, tetracycline and trimethoprim-sulfamethoxazole. The percentage of resistance isolates were presented as, Tetracycline (95.23%), Cefotaxime (85.71%), Trimethoprim- Sulfamethoxazol (83.33%), Aztreonam (71.42%), Ceftazidime (69.04%), Ciprofloxacin (59.52%), Gentamycin (26.19%), Imipenem (21.42%), Meropenem and Amikacin (19.04 %).
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Chakraborty, D., S. Basu and Das,S. A study on infections caused by Metallo-B- Lactamase Producing Gram negative Bacteria in Intensive Care Unit Patients. American Journal of infectious Diseases. 2010; 6 (2): 34-39.
Overturf, G.D. Carbapenemases: A Brief(including broad spectrum drugs such as carbapenems) Review for Pediatric. Infectious Disease Specialists. The Pediatric Infectious disease Journal .2010; 29(1): 68-70.
Pai, H.; Kang, C.I.; Byeon, J.H.; Lee, K.D.; Park, W.B. and Kim, H.B. . Epidemiology and clinical features of bloodstream infections caused by AmpCtype- beta-lactamase-producing Klebsiella pneumoniae. Antimicrob. Agents Chemother.2004; 48: 3720-3728.
Fiett, J., Baraniak, A., Mrüwka, A., Fleischer, M. Drulis-Kawa,Z., Naumiuk, L., Samet, A., Hryniewicz W. and Gniadkowski, M. . Molecular Epidemiology of Acquired-Metallo-B-Lactamase- Producing Bacteria. in Poland. Antimicrobial agents and Chemotheraapy. 2006; 50(3): 880-886.
Subha, A.; Renuka ,V. and Ananthan, S. . Department of AmpC b-lactamase producing multidrug resistant strains of Klebsiella spp. & Escherichia coli isolated from children under five in Chennai, Indian J Med Res .2003;117, 13-18.
Franco, M.R.G., Caiaffa-Filho, H.H. , Burattini, M.N. and Rossi, F. . Metallo-beta-lactamases amongimipenem-resistant Pseudomonas aeruginosain a Brazilian University hospital Clinics.2010; 65(9): 825-829.
Miriagou, V., Cornaglia,G., Edelistin , M., Galani,I., Giske, C.G.,Gniadkowski,M.,Malamou-Lada,E.,Martinez- Martinez, L.,Navarro,F.,Nordmann,P.,Pexi,L.,Papournaras, S.,Rossolini, G.M., Tsakaris, A., Vatopoulose,A., and Canton, R. . Acquired carbapenemase in Gram negative bacterial pathogenes: detection and surveillence issues. Clin. Microbial infect.2010; 16: 112-122.
Franklin, C., Liolios, L. and Peleg, A.Y. (2006). Phenotypic Detection of Carbapenem Susceptible Metallo-B-Lactamase-Producing Gram-Negative Bacilli in the Clinical Laboratory. Journal of Clinical Microbiology.2006;44(9): 3139-3144.
Franklin, C., Liolios, L. and Peleg, AY. . Phenotypic detection of carbapenem-susceptible metallo-betalactamase- producing gram negative bacilli in the clinical laboratory. J. Clin. Microbiol. 2006; 44:3139-44.
Horieh, S. ,Zohreh, K., Parviz, O., Mohammad Ali, B.and Seyed, M. . Phenotypic detection of Metallo-betalactamase- producing Pseudomonas aeruginosa strains isolated from burned patients.Iranian J. Pathol. 2008;3:20-24.
MacFaddin, J. F. . Biochemical tests for identification of medical bacteria. 3rd ed. Lippincott Williams and Wilkins.2000. USA.
Saderi, H., Zohreh, K. , Parviz, O. , Bahar,M.A., Baghr, S.M. . Phenotypic Detection of Metallo-betalactamase Producing Pseudomonas aeruginosa strains isolated from burned patients. Iranian Journal of Pathology. 2008;31: 20-24.
Samatha, P. and Parveen,K.V. . Prevalance of ESBL Ampc B-lactamase in Gram negative clinical isolates. Journals of bioscience and technology .2011;24: 353-357.
Behera, B., Mathur, P.,Das, A., Kapil, A., and Sharma, V. . An evaluation of four different phenotypic techniques for detection of metallo -lactamase producing Pseudomonas aeruginosa. Indian. J. Med. Microbiol.2008; 263: 233-237.
Supriya, U., lay R. , and Amitabha B. . Presence of different -lactamase classes among clinical isolates of Pseudomonas aeruginosa expressing AmpC -lactamase enzyme. J Infect. Dev. Ctries. 2010; 44: 239-242.
Lee, K., Y. Chong, H. B. Shin, Y. A. Kim, D. Yong, and J. H. Yum. . Modified Hodge and EDTA-disk synergy tests to screen metallo -lactamase-producing strains of Pseudomonas and Acinetobacter species. Clin. Microbiol. Infect.2001; 7:88-91.
Amjad, A., Mirza, IA., Abbasi, S.A., Farwa, U., Malik, N., and Zia, F. . Modifed Hodge test: A simple and effective test for detection of Carbapenemase production. Iran . J.Microbiol. 2011;34 : 189-193.
C.L.S.I . Performance Standards for Antimicrobial Susceptibility Testing; Twenty-First Informational SupplementClinical and Laboratory Standards Institute antimicrobial susceptibility testing standards, Clinical and Laboratory Standards Institute .2011;31.
Hansen, D.S. ; Aucken, H.M. and Abiola, T. . Recommended test panel for differentiation of Klebsiella species on the basis of a trilateral interlaboratory evaluation of 18 biochemical test. J. Clin. Microbiol.2004; 42:3665-3669.
Neuberger, A. ; Oren, I. and Sprecher, H. . Clinical impact of a PCR assay for rapid identification of Klebsiella pneumoniae in blood culture .J. Clin. Microbiol.2008; 46:377-379.
Al-Charrakh, A. H. . Bacteriological and Genetic Study on Extended-Spectrum Beta-Lactamases and Bacteriocins of Klebsiella isolated from Hilla city. Ph.D. Thesis. College of Science. Baghdad University.2005.
Kehinde, A.O. ; Ademla, S.A. ; Okesola, A.O. ; Oluwatosin, O.M. and Bakare, R.A. . Pattern of bacterial pathogens in burn wound infections in ibadan, Nigeria, Annals of burns and fire disasters.2004; vol. XVII, No. 1, pp.1-5.
Macedo, J. L. S. and Santos, J. B. . Bacterial and fungal colonization of burn wounds, Mem Inst Oswaldo Cruz, Rio de Janeiro.2005; Vol. 100, No.5, pp. 535- 539.
Amin, A. G.; Hussain, P.B. and Hameed, A. . Prevalence of antibiotic resistance among clinical isolates of Klebsiella pneumoniae isolated from a Tertiary Care Hospital in Pakistan . Malaysian Journal of Microbiology.2009; 52: 81-86.
WHO . Techniques for the detection of β – Lactamase producing strains of Neisseria gonorrhoeae.1978; 616: 137 – 143.
Charan, J., Mulla, S. , Ryavanki, S. and Naresh Kantharia, N. .New Delhi Metallo – beta lactamase – 1 containing Enterobacteriaceae: Origin, Diagnosis, Treatment and Public health concern. Pan African medical journal.2012; 1-7.
Patel G, Huprikar S, Factor SH, Jenkins SG, Calfee DP. . Outcomes of carbapenem-resistant Klebsiella pneumoniae infection and the impact of antimicrobial and adjunctive therapies. Infect Control Hosp Epidemiol.2008; 29:1099-106.
Saderi, H.; Zohreh, K. ; Parviz, O. ; Bahar,M.A. and Baghr, S.M. . Phenotypic Detection of Metallo-betalactamase Producing Pseudomonas aeruginosa strains isolated from burned patients. Iranian Journal of Pathology.2008; 31: 20-24.
Bashir, D.; Thokar,M.A.; Fomda,B.A.; Bashir,G.; Zahoor,D.; Ahmad, S. and Abubaker Toboli, S. . Detection of metallo -lactamase MBL producing Pseudomonas aeruginosa at a tertiary care hospital in Kashmir. African Journal of Microbiology Research. 2011;52: 164-172.
Eser, O.; Ergin, A. and Hasçelik, G. . Antimicrobial resistance and existence of metallo -lactamase in Acinetobacter species isolated from adult patients. Mikrobiyol Bul.2009; 433: 383-390.
Gupta, V.; Sidhu, S. and Chander, J. (2012). Metallo β-lactamase producing non fermentative Gram negative bacteria: an increasing clinical threat among hospitalized patients. Asian Pac J. Trop. Med.2012; 59: 718-721.
Metwally,L.; Gomaa,N.; Attallah,M. and Kamel,N. . High prevalence of Klebsiella pneumonia carbapenemase-mediated resistance in K. pneumonia isolates from Egypt. EMHJ .2013;19(11): 947-951.
Noyal, M. J. C.; Menezes, G. A. ; Harish, B. N. ; Sujatha, S. and Parija, S.C. . Simple screening tests for detection of Carbapenemases in clinical isolates of nonfermentative Gram-negative bacteria. Indian Journal of Medical Research. 2009;1296:707-712.
Hashemi, H. B.; M. Farzanehkhah; A. Dolatyar; M. Imani; M. R. Farzami and M. Rahbar. A study on prevalence of KPC producing from Klebsiella pneumoniae using Modified Hodge Test and CHROMagar in Iran. Annals of Biological Research.2012; 312: 5659-5664.
Al-Qafaji, M.H.M. . Biofilm Formation by The External Fixators Contaminating Klebsiella pneumoniae and Its Antibiotic Resistance. M.Sc. thesis. College of Science, University of Baghdad, Baghdad Iraq.2008.
Al-Obadi, T.H.Z. . Molecular Identification of Klebsiella pneumoniae Using Capsule Genes. M.Sc. Thesis. College of Science, Al-Nahrain University, Baghdad, Iraq.2014.
Kevin, B. L.; Sean, M.B.;Daniel, B.G.; Andrew, W.K.; Terry, R. and Dierdre, L.C. (2005). Intensive care unit- aquired urinary tract infections in regional critical care system. Critical care.2005; 9: R60-R65 .
Nicolau, D.P. . Carbapenems: a potent class of antibiotics. Expert Opin. Pharmacother.2008; 9(1): 23-37.
Abdolaziz Rastegar Lari; Leila Azimi ; Mohammad Rahbar ;Fatemeh Fallah and Reza Alaghehbandan. . Phenotypic detection of Klebsiella pneumoniae carbapenemase among burns patients: First report from Iran. Burns.2013; 39, 174 -176.
Masoume ,Bina;Abazar, Pournajaf ;Shiva Mirkalantari; Malihe Talebi and Gholamreza Irajian. . Detection of the Klebsiella pneumoniae carbapenemase (KPC) in K.pneumoniae Isolated from the Clinical Samples by the Phenotypic and Genotypic Methods. Iran. J. Path.2015; 10 (3), 199 – 205.
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