The prevalence of brucellosis of farm animals using serum- and milk- ELISA test in Al- Najaf province
DOI:
https://doi.org/10.36320/ajb/v9.i2.7602Keywords:
brucellosis, ELISA test, buffaloesAbstract
A total of 412 milk and sera samples were collected from the same animals of 88 buffaloes, 212 cattle, 84 sheep and 28 goat were screened by indirect ELISA (i ELISA) milk confirmed with serum i- ELISA. The results of seropositive i-ELISA were 121(29.36%) from all farm animals and distributed to 20 (22.27%) buffaloes, 36 (16.98%) cattle , 56(66.6%) sheep and 9 (32.14%) goat . The positive results of milk i ELISA were 89 (21.6%) from all farm animals and distributed to 8 (9.09%) , 32 (15.09%) , 44(52.83%) and 5(17.85%) represent buffaloes , cattle , sheep and goat respectively . epidemiological informations of animals collected by questionary about ( abortion , stillbirth and previous history vaccination against brucellosis) . The prevalence was relatively higher in sheep and goat followed by buffaloes and cattle . The sera and milk i ELISA test is only the best serological tests and has a high efficiency and accuracy in the diagnosis and inexpensive and can be used to control of the disease.
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Al-Khalaf, S.A., Mohamad, B.T., Nicoletti, P., 1992. Control Of Brucellosis In Kuwait By Vaccination Of Cattle, Sheep And Goats With Brucella abortus Strain 19 or Brucella melitensis strain Rev.1.Trop.Animal health Prod. 24, 45-49.
Alton, G.G. ; Nielsen, K. and Buncan, J.R. 1990. Brucella melitensis . In"Animal brucellosis". CRC press. Boston. 383-409.
Nielsen K. Diagnosis of brucellosis by serology. Vet Microbiol 2002; 90:447--459.
Blasco, J.M., Marín, C.M., Jiménez De Bagüés, M.P., Barberán, M., Hernandéz, A.,Molina, L., Velasco, J., Díaz, R., Moriyón, I., 1994. Evaluation Of Allergic And Serological Tests For Diagnosis Of Brucella melitensis in sheep and goats. Vet Rec ; 134: 415-420.
Noriello S 2004. Laboratory-acquired brucellosis- Detection of Brucella abortus in Bovine Milk by Polymerase Chain Reaction. Emerg. Infect. Dis., 10: 1848-1850.
Cho D, Nam H, Kim J, Heo E, Cho Y, Hwang I, Kim J, Kim J, Jung S 2010. Quantative Rose Bengal Test for diagnosis of bovine brucellosis. J. Immunoassay Immunochem., 31 (2): 120-30
Hunter D, Allen J 1972. An evaluation of milk and blood tests used to diagnose brucellosis. Vet. Rec., (13):310-312
McGiven, Tucker J, Perrett L, Stack BS, MacMillan A 2003. Validation of FPA and cELISA for the detection of antibodies to Brucella abortus in cattle sera and comparison to SAT, CFT and iELISA. J. immunol. 278: 171-17.
OIE, Office International des Epizooties (World organization for Animal Health) (2004). Bovine Brucellosis . Section 2.3 in OIE manual of Standards for diagnostic tests and vaccines. 5th ed. OIE Paris.
Patel T (2007). Serological, cultural and molecular detection of Brucella infection in bovine including quantification in milk by real time PCR. Masters thesis, College of Veterinary Science and Animal Husbandry, Aand Agricultural University, Anand.
Neilsen K, Smith P, Gall D, Perez B, Cosma C, Muller P, Trottier J, Cote G, Boag L, Bosse J1996: Development and validation of an indirect enzyme immunoassay for detection of antibody to Brucela abortus in milk. Vet Microbiol, 52, 165-173,.
Crespo, F. 1994 Brucellosis ovinary caprina.OIE, Paris, France.
Idrees B. A. Al-abdaly 2012.A comparative study of Rose Bengal and indirect ELISA tests for detection of the brucella antibodies in sera of cows in some areas of Salah El-Din province, Journal of Tikrit Agriculture science 12 .No.4.
Göknur Terzi , Özlem BÜYÜKTANIR , Oktay GENÇ , Ali GÜCÜKOĞLU , Nevzat YURDUSEV. 2009 Detection of Brucella Antibody and DNA in Cow Milk by ELISA and PCR Methods. Kafkas Univ Vet Fak Derg (Suppl-A): S47-S52, DOI:10.9775/kvfd..1284.
Ahmed, T.M., Bassiony.M.M . and Ibrahim , I.G.A. 2002: Field evaluation of complement fixation test and enzyme immunosorbent assay for detection of brucellosis in Bull.J.Egypt.Vet.Med.Assoc, 62(1) :119-125.
Amin KMR, Rahman MB, Rahman MS, Han JC, Park JH, Chae JS. 2005 Prevalence of Brucella antibodies in sera of cows in Bangladesh. J Vet Sci , 6, 223-226.
Fosgate, G. T.; Adesiyun, A. A.; Hird, D. W. and Hietala, S. K.( 2006). Likelihood ratio estimation without a gold standard: A case study evaluatuing a brucellosis c-ELISA in cattle and water buffalo of Trinidad. Preven. Vet. Med. , 75: 189-205.
Arslan S. H., Hussain Kh. J., Asmaeel S. A. and Hassan S. D.. 2009 Comparison between Rose Bengal and indirect ELISA tests for detection of the antibrucella antibodies in serum of sheep in Mosul city Iraqi Journal of Veterinary Sciences (21), 4, 108-113.
Abeer L. Mohammad; Esraa Ab.W. Mohammad Ali; Wesam M. Salih 2009. Investigation of antibodies of Brucella melitensis in sheep in Basra Vet.J. 11, 46-48 .
Esmaeel S. A., Mohmmed B. A., Hassan S. D. and Hassain Kh. J. 2010. Detection of anti brucella antibodies in lambs and goat kids using rose bengal test and indirect ELISA in Gugjeli - Ninavah province, Iraq. Iraqi Journal of Veterinary Sciences, Vol. 24, No. 2, (23-26).
Al-Obaidi Q. T., Hassan S. D., Mohammad B. A. and Arslan S. H. 2009. Prevalence of brucellosis using indirect ELISA test in raw milk in individual cases of ewes and does in Mosul city Iraqi Journal of Veterinary Sciences, Vol. 23, No. 2, (111-114).
Abalos P., Ibarra, L., Pinochet, L., NAVIA, F., Boisier, X., (1996) : Residual antiBrucella abortus strain 19 antibodies detected in adult cattle by two indirect-ELISA tests, Veterinary Record 138, 140.
Hussein AAA, Sayed ASM, El Feki MA. 2005 Seroepidemiological study on human brucellosis in Assiut Governorate. Egypt J Immumol;12:49-56.
Gulaand ST, Khan A. Epidemiology and Epizootology of brucellosis 2007: A review. Pakistan Vet J.;27(3):145-151.
H.A. Kaoud1, Manal.M. Zaki1, A.R. El-Dahshan1 , Shimaa1 .A. Nasr1 2010. Epidemiology of Brucellosis Among Farm Animals natural and science.;8(5)
Al-Ani FK, El-Qaderi S, Hailat NQ, Razziq R, Al- Darraji AM. 2004 Human and animal brucellosis in Jordan between 1996 and 1998: a study.Rev.Sci.Tech.23(3):831-40.
Muma JB, Samui KL, Siamudaala VM, Oloya J, Matop G, Omer MK, Munyeme M, Mubita C, Skjerve E. 2006. Prevalence of infection in traditional cattle, goats and sheep reared in livestock-wildlife interface areas of Zambia. Trop Anim Health Prod . 2006 Apr; 38(3):195-206.
Gall, D. and Nielsen , K. 2004 . Serological diagnosis of bovine brucellosis : A review of test performance and cost comparison. Rev. Sci. tech. Int Epiz. , 23 : 989-1002.
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