Control of Salmonella Spp isolated from Garmat Ali sewage by superoxide radicals produced in vitro.

Abstract

A modified method was used in the present study to generate superoxide radicals (O₂^.-) in vitro, the method involves exposing of riboflavin to lighting and generate O₂^.-. Salmonella Spp was isolated from sewage of  Garmat Ali region in Basrah city, Iraq. This study aimed to reduced Salmonella Spp by submitted it  to superoxide radicals. A significant reduction in the number of bacteria was revealed after exposed to superoxide radicals at (10, 20, 30, 40 and 50) µg/mL of riboflavin for 10 min of lighting with fluorescent  lamp ( 40 watt ), as well as the reduction in bacteria number was observed when the bacteria was exposed to superoxide radicals at 30µg/mL of riboflavin for intervals (5, 10, 15, 20, 25 and 30) min of lighting with fluorescent  lamp. This study  suggesting that reduction in bacteria number was increased as increased riboflavin concentration or increased lighting time.