MOLECULAR STUDY OF MOST COMMON PATHOGENIC BACTERIA ISOLATED FROM CONJUNCTIVITIS PATIENTS IN BAGHDAD
DOI:
https://doi.org/10.36320/ajb/v9.i3.7918Keywords:
MOLECULAR, PATHOGENIC BACTERIA, VanA geneAbstract
Aim of the study:[The current study was conducted to investigate the causes of bacterial eye infections which included inflammation of conjunctivitis, and detection of molecular study of most common pathogenic bacteriaat different age groups].
Method: study was conducted in Educational laboratories of Microbiology-Baghdad medical city,involve taken sample from Ibn al-Hatham teaching hospital,AlKindi specialized hospital and Baghdad medical city / ghazi al– Hariri teaching hospital during the period from December / 2016 to Jun / 2017.
Results: Culture investigation showed 194 positive bacterial growth of 200,103 isolate represent Gram positive,91 isolate Gram negative, and 6 no growth.Most common bacteria were Staphylococcus aureus showed high percentage [41(20.5%)], Whileother bacteria less than Staphylococcus aureus.
Conclusion:To the best of our knowledge this study was the first study in Iraq explained the molecular study of conjunctivitis patients. That concluded there 41cases infection with Staphylococcus aureusand showed all cases were methicillin resistance (MRSA), but not all carried gene mecA only [35(85.37%)], 13(31.71%) carried Panton-Valentine leukocidingene (PVL), and no one have VanA gene responsible of Vancomycin resistance..
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References
Madhavi, M., et al., Ocular morbidity among school aged children in Indian scenario. International Journal of Research in Medical Sciences, 2015.
Alash, S.A.A.A.A., Study the Prevalence of Bacterial Conjunctivitis in Iraq. Iraqi Journal of Science, 2015. 56, No.4C, pp: 3371-3375.
Jebur, K.S., Assessment of Exotoxin A and Phospholipase C Genes of Pseudomonas aeruginosa Isolated from Eye Infections. 2015.
Mondal, S., et al., A STUDY ON BACTERIOLOGICAL PROFILE OF CONJUNCTIVITIS PATIENTS ATTENDING IN A PERIPHERAL TERTIARY MEDICAL COLLEGE OF WEST BENGAL. Journal of Advanced Medical and Dental Sciences Research, 2016. 4(5): p. 5.
Derrick, T., The Role of Epigenetics and Type 2 Epithelial-Mesenchymal Transitions in Trachoma. 2016, London School of Hygiene & Tropical Medicine.
Azmi, H., Clinical study of bacterial conjunctivitis in Khartoum Eye Teaching Hospital outpatient Clinic over a period of one year. 2015, UOFK.
Dhamodaran, K., et al., Characterization of ex vivo cultured limbal, conjunctival, and oral mucosal cells: a comparative study with implications in transplantation medicine. Molecular vision, 2015. 21: p. 828.
Gunduz, G., et al., The Effect of Chronic Alcoholism on the Conjunctival Flora. Current eye research, 2016. 41(6): p. 734-739.
Rhoades, W., L. Kump, and E. Margalit, Anterior Chamber and Retina (Structure, Function and Immunology), in Neuroimmune Pharmacology. 2017, Springer. p. 39-54.
Waldenburger, S., et al., Community‐acquired skin infections caused by Staphylococcus aureus: What is the role of the Panton‐Valentine leukocidin toxin? JDDG: Journal der DeutschenDermatologischenGesellschaft, 2014. 12(1): p. 59-66.
Wang, N., et al., Bacterial spectrum and antibiotic resistance patterns of ocular infection: differences between external and intraocular diseases. Journal of ophthalmology, 2015. 2015.
Dunlop, A.L. and J.R. Wells, Approach to Red Eye for Primary Care Practitioners. Primary Care: Clinics in Office Practice, 2015. 42(3): p. 267-284.
rzybowski, A., P. Brona, and S.J. Kim, Microbial flora and resistance in ophthalmology: a review. Graefe's Archive for Clinical and Experimental Ophthalmology, 2017: p. 1-12.
Ni, N., et al., Seasonal, geographic, and antimicrobial resistance patterns in microbial keratitis: 4-year experience in eastern Pennsylvania. Cornea, 2015. 34(3): p. 296-302.
Andalibi, S., et al., An update on neonatal and pediatric conjunctivitis. Current Ophthalmology Reports, 2015. 3(3): p. 158-169.
Ogston, A., Micrococcus poisoning. Journal of anatomy and physiology, 1882. 16(Pt 4): p. 526.
Chan, A.H., et al., Discovery of Staphylococcus aureus sortaseA inhibitors using virtual screening and the relaxed complex scheme. Chemical biology & drug design, 2013. 82(4): p. 418-428.
Orenstein, A., The discovery and naming of Staphylococcus aureus. 2011.
Miller, D., et al., Comparative in vitro susceptibility of besifloxacin and seven comparators against ciprofloxacin-and methicillin-susceptible/nonsusceptible staphylococci. Journal of Ocular Pharmacology and Therapeutics, 2013. 29(3): p. 339-344.
Vola, M.E., et al., Prevalence and antibiotic susceptibility of methicillin-resistant Staphylococcus aureus in ocular infections. Arquivosbrasileiros de oftalmologia, 2013. 76(6): p. 350-353.
Morse, S., K.C. Carroll, and J. Butel, JawetzMelnick&Adelbergs Medical Microbiology 27 E. 2015: McGraw-Hill Education. 742.
Jeaz, I.T., Investigation for some virulance factors of some Bacterial pathogenic Causes Isolated and characterized from Conjunctivitis states and sensitivity of Dominant Bacteria for some Antibiotics. . journal of al-qadisiyah for pure science(quarterly) 2012. 17(4): p. 1-12.
Majedkadem, A.L.S., Isolation of bacteria that cause eye infection and Identification Diwaniya city journal of al-qadisiyah for pure science(quarterly) 2012. 17(3): p. 1-12.
Alsamarai, A.M., H.M. Abbas, and Q.M. Atia, Nasal carriage of methicillin resistant Staph aureus in food provider in restaurant at Samara city. World Journal of Pharmacy and Pharmaceutical Sciences (WJPPS), 2015. 4: p. 50-58.
El Aila, N.A., N.A. Al Laham, and B.M. Ayesh, Nasal carriage of methicillin resistant Staphylococcus aureus among health care workers at Al Shifa hospital in Gaza Strip. BMC infectious diseases, 2017. 17(1): p. 28.
Rimland, D., Nosocomial infections with methicillin and tobramycin resistant Staphylococcus aureus—implication of physiotherapy in hospital-wide dissemination. The American journal of the medical sciences, 1985. 290(3): p. 91-97.
Li, B. and T.J. Webster, Bacteria Antibiotic Resistance: New Challenges and Opportunities for Implant‐Associated Orthopaedic Infections. Journal of Orthopaedic Research, 2017.
Mokhtar, A.A.-H., I.A.-M. Khalid, and T.A.-H. Hadeel, INCIDENCE OF METHICILLIN RESISTANT STAPHYLOCOCCI IN BACTERIAL CONJUNCTIVITIS. Basrah Journal of Surgery 2006. 12(1): p. 98-99.
D'Souza, N., C. Rodrigues, and A. Mehta, Molecular characterization of methicillin-resistant Staphylococcus aureus with emergence of epidemic clones of sequence type (ST) 22 and ST 772 in Mumbai, India. Journal of clinical microbiology, 2010. 48(5): p. 1806-1811.
Ruimy, R., et al., The carriage population of Staphylococcus aureus from Mali is composed of a combination of pandemic clones and the divergent Panton-Valentine leukocidin-positive genotype ST152. Journal of bacteriology, 2008. 190(11): p. 3962-3968.
Schaumburg, F., et al., Population structure of Staphylococcus aureus from remote African Babongo Pygmies. PLoS neglected tropical diseases, 2011. 5(5): p. e1150.
Kaur, H., et al., Status of methicillin-resistant Staphylococcus aureus infections and evaluation of PVL producing strains in Belgaum, South India. JKIMSU, 2012. 1(2): p. 43-51.
Al-Mohana, A.M., et al., Community-acquired methicillin-resistant Staphylococcus aureus carrying mecA and Panton-Valentine leukocidin (PVL) genes isolated from the holy shrine in Najaf, Iraq. African Journal of Bacteriology Research, 2012. 4(2): p. 15-23.
Alobaidy, R.A.A., Molecular characterization of Panton Valentine leukocidine and Exfoliatine toxins in local isolates of Methicillin resistant Staphylococcus aureus in Biology/Microbiology
, Al-Mustansiriyah University /College of Science.
Oksuz, L., et al., The high diversity of MRSA clones detected in a university hospital in Istanbul. International journal of medical sciences, 2013. 10(12): p. 1740.
Zahan, N., et al., Paul SK", Ahmed S", Sumona AA”, Begum Z", Alam M", YusufMA", Uddin MS. Mymensingh Med J, 2009. 18(1): p. 21-26.
Reddy, P., et al., Postpartum mastitis and community-acquired methicillin-resistant Staphylococcus aureus. Emerging infectious diseases, 2007. 13(2): p. 298.
Yaren, H., et al., Investigation of PVL and MecA Gene Locies in Methicillin-Resistant Staphylococcus aureus (MRSA) Strains Isolated from Nosocomial Samples of Hospital Patients and Hospital Staff. Genomics and Applied Biology, 2015. 6.
Abdelgadeir, L.M.A., Detection
and Molecular Characterization of Methicillin and Vancomycin Resistant Staphylococcus aureus and Sequencing of mecA and arcC Genes among Different Clinical Isolates from Shendi City. 2015, جامعة شندي.
Mohammed Khalifah , I.M., Study the normal flora of eye conjunctivitis for healthy persons and effect
ofChloromphenicol antibiotic in Diyala Governorate. Tikrit Journal of Pure Sciences, 2013 18(4): p. 74 -76
Abid, A.J. and R.M. Ewadh, Etiology of Bacterial Eye Infections and Determination of Immune Response of Infected Patient. Medical Journal of Babylon, 2012. 9(4): p. 799-805.
Ubani, U., Common bacterial isolates from infected eyes. Journal of the Nigerian Optometric Association, 2009. 15(1): p. 40-47.
Thewaini, Q.N., Cryptic and Non cryptic Bacterial Ophthalmitis. Journal of Babylon University/Pure and Applied Sciences, 2014.22(6).
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