ISOLATION AND PURIFICATION OF STAPHYLOCOCCUS AUREUS SURFACE ANTIGEN B AND USING IT FOR THE DIAGNOSIS BY ELISA TECHNIQUE
DOI:
https://doi.org/10.36320/ajb/v9.i3.7920Keywords:
STAPHYLOCOCCUS AUREUS , ELISA TECHNIQUE, enterotoxin BAbstract
Staphylococcus aureus is the most common cause of staphylococcal infections. It is a spherical bacterium, frequently lives on the skin or in the nose of a human.
Many tests were done for the diagnosis of Staphylococcus aureus isolated from blood culture, these include: culture, gram stain, catalase test, coagulase test and diagnosis by Vitek system. The results appear that 90% were (H7) isolates while 10% were other isolate.
Chromatography technique was used for extraction, purification and isolation of the antigen (enterotoxin B) from the cell wall of Staphylococcus aureus.
ELISA technique was used for the detection of enterotoxin B.
This antigen (enterotoxin B) was employed for the production of diagnostic ELISA kit against staphylococci (particularly for Iraqi peoples).
Downloads
References
Ahanotu, E., Alvelo-Ceron, D., Ravita, T. and Gaunt, E., 2006. Staphylococcal enterotoxin B as a biological weapon: recognition, management, and surveillance of staphylococcal enterotoxin. Applied Biosafety, 11(3), pp.120-126.
Argudín, M.Á., Mendoza, M.C. and Rodicio, M.R., 2010. Food poisoning and Staphylococcus aureus enterotoxins. Toxins, 2(7), pp.1751-1773.
Bonar, E., Wójcik, I. and Wladyka, B., 2015. Proteomics in studies of Staphylococcus aureus virulence. Acta Biochimica Polonica, 62(3), pp.367-381.
Coates, R., Moran, J. and Horsburgh, M.J., 2014. Staphylococci: colonizers and pathogens of human skin. Future microbiology, 9(1), pp.75-91.
Fooladi, A.I., Tavakoli, H.R. and Naderi, A., 2010. Detection of enterotoxigenic Staphylococcus aureus isolates in domestic dairy products. Iranian journal of microbiology, 2(3), p.137.
Fries, B.C. and Varshney, A.K., 2013. Bacterial Toxins—Staphylococcal Enterotoxin B. Microbiology spectrum, 1(2).
Kientz, C.E., Hulst, A.G. and Wils, E.R., 1997. Determination of staphylococcal enterotoxin B by on-line (micro) liquid chromatography-electrospray mass spectrometry. Journal of chromatography A, 757(1-2), pp.51-64.
Kong, C., Neoh, H.M. and Nathan, S., 2016. Targeting Staphylococcus aureus toxins: a potential form of anti-virulence therapy. Toxins, 8(3), p.72.
Kuang, H., Wang, W., Xu, L., Ma, W., Liu, L., Wang, L. and Xu, C., 2013. Monoclonal antibody-based sandwich ELISA for the detection of staphylococcal enterotoxin A. International journal of environmental research and public health, 10(4), pp.1598-1608.
Muratovic, A.Z., Hagström, T., Rosén, J., Granelli, K. and Hellenäs, K.E., 2015. Quantitative analysis of Staphylococcal enterotoxins A and B in food matrices using ultra high-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS). Toxins, 7(9), pp.3637-3656.
Ortega, E., Abriouel, H., Lucas, R. and Gálvez, A., 2010. Multiple roles of Staphylococcus aureus enterotoxins: pathogenicity, superantigenic activity, and correlation to antibiotic resistance. Toxins, 2(8), pp.2117-2131.
Peterson, M.L., Ault, K., Kremer, M.J., Klingelhutz, A.J., Davis, C.C., Squier, C.A. and Schlievert, P.M., 2005. The innate immune system is activated by stimulation of vaginal epithelial cells with Staphylococcus aureus and toxic shock syndrome toxin 1. Infection and immunity, 73(4), pp.2164-2174.
Schelin, J., Wallin-Carlquist, N., Thorup Cohn, M., Lindqvist, R. and Barker, G.C., 2011. The formation of Staphylococcus aureus enterotoxin in food environments and advances in risk assessment. Virulence, 2(6), pp.580-592.
Wu, S., Duan, N., Gu, H., Hao, L., Ye, H., Gong, W. and Wang, Z., 2016. A Review of the Methods for Detection of Staphylococcus aureus Enterotoxins. Toxins, 8(7), p.176.
Zecconi, A. and Scali, F., 2013. Staphylococcus aureus virulence factors in evasion from innate immune defenses in human and animal diseases. Immunology letters, 150(1), pp.12-22.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2017 Areej A. Mohammed, Ali A. Mahdi
This work is licensed under a Creative Commons Attribution 4.0 International License.
which allows users to copy, create extracts, abstracts, and new works from the Article, alter and revise the Article, and make commercial use of the Article (including reuse and/or resale of the Article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made and the licensor is not represented as endorsing the use made of the work.
