Isolation of biosurfactant/bioemulsifier from Saccharomyces cerevisiae by different treatment
DOI:
https://doi.org/10.36320/ajb/v9.i3.7921Keywords:
Biosurfactan, Bioemulsifier, Saccharomyces cerevisiaeAbstract
Biosurfactant are amphiphilic compound have been increasingly attracting the attention of the scientific community as promising candidates for the replacement of a number of synthetic surfactants. The aim of this study is to extraction of Biosurfactant from Saccharomyces cerevisiae by using three treatment (absolute ethanol , ammonium sulphate and heating). The present study clearly showed that , the treatment with boiling to 30 min was very effect to extract the biosurfactant from S. cerevisiae best than other treatments with high emulsification index and surface activity where were 81.08% and 20 mm , respectively
Downloads
References
Alcantara , V. A.; Pajares , I. G.; Simbahan , G. F. and Rubio , M. L. D. (2011). "Substrate Dependent Production and Isolation of an Extracellular Biosurfactant from Saccharomyces cerevisiae 2031 ". (2012, June ). Philippine Journal of Science. 141 (1): 13-24.
Barnett , J. A.; Payne, R. W. and Yarrow, D. (1985). Characteristic and Identification In : Yeast.2nded.,Cambridge University Press .pp.8-45.
Burgess, R. R. (2009). Deutscher, Richard R. Burgess and Murray P., ed. Methods in Enzymology. Guide to Protein Purification, 2nd Edition. 463, 331–342.
Dhivya , H.; Balaji , S.; Madhan , R. and Srv , A. (2014). Production of Amphiphilic Surfactant Molecule From Saccharomyces Cerevisiae Mtcc 181 And Its Protagonist In Nanovesicle Synthesis. (2014, November) International Journal of Pharmaceutical Science Invention. 3 (11):16-23.
Dikit, P.; Maneerat, s.; Musikasang, H. and H-kittikun, A. (2009). Emulsifier properties of the mannoprotein extract from yeast isolated.(October, 2010). Science Asia. 36 : 312–318.
Farahnejad , Z. J.; Rasaee, M.; Yadegari, H. and Moghadam, M. F. (2004). Purification and characteriazation of cell wall mannoproteins of Candida albicans using intact cell method. (2004, August). Medical Journal of the Islamic Republic of Iran. 18(2):167-172.
Fracchia , L.; Cavallo,M.; Martinotti, M.G.; and Banat, I.M. (2012). Biosurfactants and Bioemulsifiers Biomedical and Related Applications –Present Status and Future Potentials. (2012, 12 January). Biomedical Science, Engineering and Technology. 325-371.
Herrero , M. B.; de Lamirande, E. and Gagnon, C. (1999). Nitric oxide regulates human sperm capacitation and protein-tyrosine phosphorylation in vitro. (1999, September ). Biology of Reproduction. 61: 575-58.
Lodder, J. (1970). The Yeast , A Taxonomic study , North-Holland.
Luna , J. M.; Rufino, R. D.; Sarubbo, L. A.; M. Rodrigues, L. R.; Teixeira, G, A. C. and Campos-Takaki, G. M. (2011). Evaluation Antimicrobial and Antiadhesive Properties of the Biosurfactant Lunasan Produced by Candida sphaerica UCP 0995. (2011, February). Current Microbiology. DOI 10.1007/s00284-011-9889-1.
Morikawa , M.; Hirata , Y. and Imanaka , T. (2000). A study on the structure-function relationship of lipopeptide biosurfactants. (2000, Novomber). Biochimica et Biophysica Acta. 1488 (3): 211–218.
Prabakaran , M. and Sumathi, R. (2014). Studies on the biosurfactant producing pseudomonas aeuroginosa and its antimicrobial activity on selected human pathogens.(2014). International Journal of Current Research in Chemistry and Pharmaceutical Sciences. 1 (2) 69-78.
Shoep , E.; Ahmed, N.; Akhter, J.; Badar, U.; Siddiqui, K.; Ansari, F. A.; Waqar, M.; Imtiaz, S.; Akhtar, N.; Shaikh, Q. A.; Baig, R.; Butt, S.; Khan, S.; Khan, S.; Hussain, S.; Ahmed, B. and Ansari, M. A. (2014). Screening and characterization of biosurfactant-producing bacteria isolated from the Arabian Sea coast of Karachi. (2015). Turkish Journal of Biology. 39: 210-216.
Sridhar , B.; Karthik, R.; Tamil , V. M. and Khanna, G. (2013). Production, Purification And Antimicrobial Activity Of Biosurfactants From Saccharomyces Cerevisiae And Pseudomonas Aeruginosa. (2013, December). International Journal of Advance Research in Pharmaceuticals and Bioscience .3(4), 1-7.
Trindade , J. R.; Freire , M. G.; Amaral , P.F.F; Coelho , M. A. Z.; Coutinho , J. A. B. and Marrucho , I. M. (2008). Aging mechanisms of oil-in-water emulsions based on a bioemulsifier produced by Yarrowia lipolytica . (2008, July). Colloids and Surfaces A: Physicochemical
and Engineering Aspects. 324 ,149–154.
Vidhya , V.; Vidhya , A.; Gowri , B. V. and Arunadevi , S. (2015). Utilization of Synthetic Dairy Waste Water and Waste Oil for the Production of Sophorolipid from Starmerella bombicola MTCC 1910 and Testing its Antimicrobial Activity.(2015). International Journal of Current Microbiology and Applied Sciences. 4(12): 555-565.
Walenckaa , E.; Wieckowska-Szakiela ,M.; Rozalskab , S.; Sadowskaa , B. and Rozalskaa , B. (2007). A Surface-Active Agent from Saccharomyces cerevisiae Influences Staphylococcal Adhesion and Biofilm Development . (2007, November). http://www.znaturforsch.com .62, 433D438.
Walter, V., Syldatk, C., Hausmann, R. 2010. Screening Concepts for the isolation of biosurfactant producing microorganisms. ( Journal of Bioscience and Technology. 1: 120–126.
Whitaker , J. R. (1972). Principles of Enzymology for the Food Science. (ed. Oen, R. F. ) Marcel Dekker INC. New York.
Willem , E. (2012). Evaluation of the reduction of Candida albicans biofilm growth with biosurfactant AC7 in combination with antifungal drugs. Thesis. Department of Pharmaceutical Analysis .Ghent university. Belgium.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2017 Duaa Haider Raham, Nibras Nazar Mahmood
This work is licensed under a Creative Commons Attribution 4.0 International License.
which allows users to copy, create extracts, abstracts, and new works from the Article, alter and revise the Article, and make commercial use of the Article (including reuse and/or resale of the Article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made and the licensor is not represented as endorsing the use made of the work.