Fingerprint and Molecular Identification of Some Iraqi Date Palm Cultivars Using Inter-Simple Sequence Repeat (ISSR) Markers.
DOI:
https://doi.org/10.36320/ajb/v7.i2.8012Keywords:
Iraqi Date Palm , ISSR, fingerprintingAbstract
many of moleculer markers used to determination the fingerprint and diversity in date palm. the ISSR markers are more sutable and effeciency to use for this aim, in this paper we are used a seven ISSR markers to find the fingerprinting and diversity for seven Iraqi date palm, which are not mentioned in auther papers by this teqniq, after the DNA extraction from young leaves with reliable quality and quantity, the genomic DNA and ISSR markers were amplification by PCR Special program, then a DNA fragment amplification cultivers were electrophoresis, then calculating the number of mono- and polymorphic bands, which used to calculat a similarity and genetic distance between cultivar, then it are used to build a phylogenetic tree and cluster analysis.
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Copyright (c) 2019 Ahmed Usaff Lafta, Aqeel Hadi Abdulwahid, Ali Hussan Ali
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