Influence of various bioprocess factors on production of lipase from Pseudomonas aeruginosa

Authors

  • Baydaa Abood Hassan
  • Nibras yahya alsalami
  • Musa Nima Mezher

DOI:

https://doi.org/10.36320/ajb/v9.i1.8021

Keywords:

Lipase; Pseudomonas aeruginosa; production

Abstract

This study was conducted in the laboratories  of  biology department, faculty of  Science,  which deal with isolation of lipase and optimization of process parameters to achieve maximum yield of lipase by Pseudomonas aeruginosa which carried out for enhanced production of lipase using olive oil as the substrate of enzyme , maximum activity of the enzyme was achieved under best growth conditions , the best conditions were the isolated of  lipase from Pseudomonas aeruginosa in synthetic medium , it gave high titer of lipase activity , the glucose as carbon source , peptone as nitrogen source , incubation period 48 h , incubation temperature 40 °C and pH = 8 .

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References

Joseph, B., P.W. Ramtekeand and G. Thomas, (2008) . Cold active microbial lipases: Some hot issues and recent developments. Biotech Advances, 26: 457-470. DOI: https://doi.org/10.1016/j.biotechadv.2008.05.003

Snellman, E.A., E.R. Sullivan and R.R. Colwell, (2002) .Purification and properties of the extracellular lipase, Lip A, of Acinetobacter sp. RAG-1. FEBS J., 269: 5771-5779. DOI: https://doi.org/10.1046/j.1432-1033.2002.03235.x

Falch, E.A. and E.A. Falch, (1991) . Industrial enzymes-developments in production and application. Biotechnol Adv., 9: 643-658. DOI: https://doi.org/10.1016/0734-9750(91)90736-F

Fuji, T., T. Tatara and M. Minagawa, (1986) . Studies on application of lipolytic enzyme in detergent industries. J. Am. Oil Chem. Soc., 63: 796-799. DOI: https://doi.org/10.1007/BF02541967

Sharma, R., Y. Christi and U.C. Banerjee, (2001) . Production, purification, characterization and application of lipases. Biotech Advances, 19: 627-662. DOI: https://doi.org/10.1016/S0734-9750(01)00086-6

Linko, Y.Y., M. Lamsa, X. Wu, E. Uosukainen, J. Seppala and P. Linko,( 1998) . Biodegradable products by lipase biocatalysis. J. Biotechnol., 66(1): 41-50. DOI: https://doi.org/10.1016/S0168-1656(98)00155-2

Fariha H., Aamer Ali shah and Abdul Hameed, (2006) . Industrial applications of microbial lipases. Enzyme and Microbial Tech., 39(2): 235-251. DOI: https://doi.org/10.1016/j.enzmictec.2005.10.016

Eugene, W.S., (1974) . Industrial application of microbial lipases: A review. J. Am. Oil Chem. Soc., 51(2): 12-16. DOI: https://doi.org/10.1007/BF02545206

Bajpai, P., (1999) . Application of enzymes in the pulp and paper industry. Biotechnol Progr, 15: 147-157. DOI: https://doi.org/10.1021/bp990013k

Noureddini, H., X. Gao and R.S. Philkana, (2005) . Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil. Bioresour Technol., 96: 769-77. DOI: https://doi.org/10.1016/j.biortech.2004.05.029

Nakamura, K. and T. Nasu, (1990) . Enzyme Containing Bleaching Composition, Japanese Patent, 2: 208-400.

Higaki, S. and M. Morohashi, (2003) . Propionibacterium acnes lipase in seborrheic dermatitis and other skin diseases and Unsei-in. Drugs Exp. Clin Res., 29: 157-9.

Macfaddin ,J.F . (2000) . Biochemical test for identification of medical bacteria . 2 ed . William and Wilkins . Comp. Baltimore , p.490 .

. Cheng , LI. C and Chen. C , T, . (2004). Fed- batch production of lipase by Acinetobacter radioresistens using tween 80 as carbon source. Biochem. Eng. J., 19(1) , 25,31 . DOI: https://doi.org/10.1016/j.bej.2003.09.006

Borkar , PS ., Bodade RG, Rao SR, Khobragade CN . (2009). Purification and characterization of extracellular lipase from a new strain Pseudomonas aeruginosa SRT9. Braz. J. Micobiol. 40: 358-366. DOI: https://doi.org/10.1590/S1517-83822009000200028

Syed , MN. , Iqbal S, Bano S, Khan AB, Ali-ul-Qader S, Azhar A (2010). Purification and characterization of 60 kD lipase linked with chaperonin from Pseudomonas aeruginosa BN-1. Afr. J. Biotechnol. 9: 7724-7732 .

Bertrand , TF, Fredric T, Robert N (2004). Production and partial characterization of a thermostable amylase from Ascomycetes yeast strain isolated from starchy soil. McGraw-Hill Inc., New York, pp. 53-55.

Ellaiah, P. ; Adinarayana, K.; Bhavani, Y.; Padmaja , P.and Srinivasulu , B. (2002) . Optimization of process parameters for glucoamylase production under solid state fermentation by a newly isolated Aspergillus species. Process Biochem ., 38 : 615–620. DOI: https://doi.org/10.1016/S0032-9592(02)00188-7

Veerapagu , M. , DR .A. Sankara Narayanan, K.Ponmurugan, K.R.Jeya . (2013) . Scrrening selection identification production and optimization of bacterial lipase from oil spilled soil . Asian J Pharm Clin Res., Vol. 6, Suppl 3, 62-67 .

Sirisha , E. M. Lakshmi Narasu , and N. Rajasekar. (2010 ) . Isolation and Optimization of Lipase Producing Bacteria from Oil Contaminated Soils. Advances in Biological Research.; 4 (5): 249-252.

Ghaima , K., K.; Ahmed Isam Mohamed, Mahir Mahmoud Mohamed. (2014) . Effect of some factors on lipase production by Bacillus cereus isolated from diesel fuel polluted soil. International Journal of Scientific and Research Publications, Volume 4,.

. Bertolin , W. ; Schmidell , A.; Maiorano, J.; Casara, J.. (2003) . Influence of carbon , nitrogen and phosphorus sources on glucoamylase production by Aspergillus awamori in solid-state fermentation . Z. Naturforsch . pp : 708– 712. DOI: https://doi.org/10.1515/znc-2003-9-1020

KhemikaLomthaisong, Angkhameen Buranarom and Hataichanoke Niamsup. (2012) . Investigation of Isolated Lipase Producing Bacteria from Oil-contaminated Soil with Proteomic Analysis of its Proteins Responsive to Lipase Inducer. Journal of BiologicalSciences ;12:161-167 DOI: https://doi.org/10.3923/jbs.2012.161.167

Copeland , R. A. (1994) . Methods for protein analysis: a practical guide to Laboratory Protocols , Chapman & Hall , New York.

Vishwe , V.S., Vaidya S.P. and Chowdhary A.S. (2015) . Optimization of Fermentation parameters for Enhanced production of Lipase from lipolytic Pseudomonas spp. International Research Journal of Biological Sciences Vol. 4(6), 16-21.

Mobarak-Qamsari, E, R Kasra-Kermanshahi, and Z Moosavi-nejad . (2011) . Isolation and identification of a novel, lipase-producing bacterium, Pseudomnas aeruginosa KM110Iran J Microbiol.; 3(2): 92–98.

Balwinder , Singh Sooch and Baljinder Singh Kauldhar . (2013) . Influence of Multiple Bioprocess Parameters on Production of Lipase from Pseudomonas sp. BWS-5 . Braz. Arch. Biol. Technol. v.56 n.5: pp. 711-721. DOI: https://doi.org/10.1590/S1516-89132013000500002

Sangeetha, R ; A Geetha, I Arulpandi. (2007) .Optimization of protease and lipase production by Bacillus pumilus SG 2 isolated from an industrial effluent. The Internet Journal of Microbiology. Vol( 5 ) ;Number 2. DOI: https://doi.org/10.5580/2126

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Published

2017-04-01

How to Cite

Abood Hassan, B., yahya alsalami, N., & Nima Mezher, M. (2017). Influence of various bioprocess factors on production of lipase from Pseudomonas aeruginosa. Al-Kufa University Journal for Biology, 9(1), 25-32. https://doi.org/10.36320/ajb/v9.i1.8021

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