The correlation between Lactate dehydrogenase activity and sperm concentration in the seminal plasma of infertile men.
DOI:
https://doi.org/10.36320/ajb/v9.i1.8024Abstract
This study included 43 semen samples collected from sub-fertile and 10 samples of fertile men, after the abstinence period (3 to 5 days); it was conducted in the laboratory of Babylon Maternity and children, and private laboratories during the period from October 2016 to March 2017.
The study was aimed to expose for reasons of unexplained infertility in most cases, through valued the correlation between LDH activity and sperm concentration in the seminal plasma of infertile men and in comparison it with fertile men.
The results revealed significant differences (P˂0.05) in an average of the activity level of Lactate dehydrogenase enzyme between the different groups, the highest level was shown in the groups of fertile and normospermia and the lowest level in the oligospermia and oligo-asthenospermia, also revealed a significant negative correlation (P˂0.05) between LDH activity and sperm concentration in the seminal plasma of oligo-asthenospermia, the negative correlation observed in the group of normospermia and oligospermia while fertile men has positive correlation.
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- Talluri, T.R.; Mal, G. and Ravi, S.K. (2017) Biochemical components of seminal plasma and their correlation to the fresh seminal characteristics in Marwari stallions and Poitou jacks. Veterinary World; 10(2): 214-220. DOI: https://doi.org/10.14202/vetworld.2017.214-220
- Juyena, N.S. and Stelletta, C. (2012) Seminal plasma: An essential attribute to spermatozoa. J. Androl., 33(4): 536-551. DOI: https://doi.org/10.2164/jandrol.110.012583
- Lott, J.A. and Nemensanszky, E. (1987) Lactate Dehydrogenase. In: Lott, J.A., Wolf, P.L. and Sawhney, A.K., Eds., Clinical Enzymology: A Case-Oriented Approach, Field and Rich/Year Book, New York, PP:213-244.
- Goldberg, E. (1985). Reproductive implications of LDH-C4 and other testisspecific isozymes. Exp. Clin. Immunogenet.; 2(2):120–124.
- Eliasson, R.; Haggman, K. and Wikund, B. (1967). Lactate dehydrogenase in human seminal plasma. Scand. J. clin. lab. Invest.20 (4): 353-359. DOI: https://doi.org/10.3109/00365516709076965
- Amadou, C.F.; Sylvère, N.K.; Eric, A.; Noël,C.; Guillaume, Y.Y. and Joseph, D.A. (2014). Comparative Study of Enzymatic Markers in Seminal Liquids of Normospermic and Azoospermic Samples at Pasteur Institute of Cote D’Ivoire (IPCI). American Journal of Phytomedicine and Clinical Therapeutics; 2(11):1252-1261.
- Jungwirth, A.; Diemer, T.; Dohle, G.R.; Giwercman, A. ;Kopa, Z.; Krausz, C. and Tournaye, H.( 2012 ) Guidelines for The Investigation and Treatment of Male Infertility .Male Infertility ;61(1):176-188.
- World Health Organization (2010). WHO laboratory manual for the Examination and processing of human semen, 5th ed. Cambridge, Cambridge University Press.
- George, A.; Darren, G.; Mallery, D. and Paul, C. (2003). SPSS for windows step by step. Boston, Pearson Education. Inc.pp:55-56.
- Virji, N. (1985) LDH-C4 in human seminal plasma and its relationship to testicular function. I. Methodological aspects. Int. J. Androl.; 8(3):193-200. DOI: https://doi.org/10.1111/j.1365-2605.1985.tb00834.x
- Guerin, J.F.; Rollet, J.; Perrin, P.; Menezo, Y.; Orgiazzi, A. and Czyba, J.C. (1981) Enzymes in the seminal plasma from azoospermic men: correlation with the origin of their azoospermia. Fertil Steril, 36(3): 368-372. DOI: https://doi.org/10.1016/S0015-0282(16)45740-7
- Goldberg, E. (1977) Isozymes in testes and spermatozoa. In: Rattazzi M. S, Scandalios J. and Whitt G. S. (eds). Isozymes: Current Topics in Biological and Medical Research. Alan R. Liss Inc, New York. 1: 79-124.
- Feng, Rui-Xiang.; Lu, Jin-Chun.; Zhang, Hong-Ye. and Lü, Nian-Qing (2015) A Pilot Comparative Study of 26 Biochemical Markers in Seminal Plasma and Serum in Infertile Men. BioMed Research International pp 1-7. DOI: https://doi.org/10.1155/2015/805328
- Asadpour, R. (2012). Relationship between mineral compo¬sition of seminal plasma and semen quality in various ram breeds. Acta. Scientiae Veterinariae ;40 (2):1027.
- Ruiz‐Pesini, E.; Diez‐Sanchez, C.; Lopez‐Perez, M.J. and Enriquez, J. A.(2007) The Role of the Mitochondrion in Sperm Function: Is There a Place for Oxidative Phosphorylation or is this a Purely Glycolytic Process?. Current Topics in Developmental Biology, 77: 3-19. DOI: https://doi.org/10.1016/S0070-2153(06)77001-6
- Mukai, C. and Okuno, M. (2004). Glycolysis plays a major role for adenosine triphosphate supplementation in mouse sperm flagellar movement. Biol. Reprod; 71(2):540-547. DOI: https://doi.org/10.1095/biolreprod.103.026054
- Butt,F. and Akram,N. (2013). Semen analysis parameters: Experiences and insight into male infertility at a tertiary care hospital in Punjab. J. Pak. Med. Assoc.; 63(5):558-563.
- Hereng, T.H.; Elgstoen, K.B.P.; Cederkvist, F.H.; Eide, L.; Jahnsen, T.; Skalhegg, B.S. and Rosendal, K.R. (2011). Exogenous pyruvate accelerates glycolysis and promotes capacitation in human spermatozoa. Human Reproduction, 26(12) :3249 3263. DOI: https://doi.org/10.1093/humrep/der317
- Owen, D.H. and Katz, D. F. (2005). A Review of the Physical and Chemical Properties of Human Semen and the Formulation of a Semen Simulant. Journal of Andrology, 26(4):459-469. DOI: https://doi.org/10.2164/jandrol.04104
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