Molecular study of Acinetobacter baumannii Isolated from skin infection
DOI:
https://doi.org/10.36320/ajb/v11.i1.8033Keywords:
blaNDM-1, blaTEM, blaSHV, Acinetobacter baumanniiAbstract
Eight isolates of Acinetobacter baumannii were identified among one hundred fifty of Gram-negative bacteria grown on MacConkey agar that was isolated from different clinical skin specimens in Al-Najaf Al- Ashraf province hospitals. A.baumannii identification depends on morphological ,microscopic examination and biochemical tests and confirmed by Api20E .The results showed that 60% of all A.baumannii isolates produce biofilm and carry csuE gene while 40% of all A.baumannii isolates carry ompA gene .
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Ramirez,M.S.;Adams,M.D.;Bonomo,R.A.;Centron,D.andTolmasky,M.E. (2011). Genomic Analysis of Acinetobacter baumannii A118 by Comparison of Optical Maps: Identification of Structures Related to Its Susceptibility Phenotype. Antimicrob .agents chemother.5 :1520–1526.
Aydemir, H.; Celebi, G.; Piskin,N.; Oztoprak, N.; Keskin, A. S.; Aktas, E.; Sumbuloglu, V. and Akduman, D. (2012). Mortality Attributable to Carbapenem-Resistant Nosocomial Acinetobacter baumannii Infections in a Turkish University Hospital. Jpn. J. Infect. Dis. 65: 66-71.
Endo, S.; Sasano, M.; Yano, H.; Inomata, S.; Ishibashi, N.; Aoyagi, T.; Hatta, M.; Gu, Y.; Yamada, M.; Tokuda , K.; Kitagawa , M.; Kunishima , H.; Hirakata, Y. and Kaku, M. (2012). IMP-1-producing carbapenem-resistant Acinetobacter ursingii from Japan. J. Antimicrob. Chemother. 67(10): 2533-2538.
Boulanger, A.; Nass, T.; Fortineau, N.; Figueiredo, S. and Nordmann, P. (2012). NDM-1-Producing Acinetobacter baumannii from Algeria. Antimicrob. Agents Chemother. 56(4): 2214-2215.
Karah, N.; Sundsfjord, A.; Towner, K. and Samuelsen, O. (2012). Insights into the global molecular epidemiology of carbapenem non-susceptible clones of Acinetobacter baumannii. Micro. Drug Resist. 15(4): 237-247.
Choi, C.H.; Hyun, S.H.; Lee ,J.Y.; Lee, J.S.; Lee, Y.S.; Kim, S.A.; Chae, J.P. ;Yoo, S.M.and Lee, J.C.( 2008a).Acinetobacter baumannii outer membrane protein A targets the nucleus and induces cytotoxicity. Cell Microbiol, 10:309-319.
Tavajjohi, Z. and Moniri, R.(2011). Detection of ESBLs and MDR in Pseudomonas aeruginosa in a tertiary-care teaching hospital. Iranian J. Clin. Infect. Dis..6:18-23.
Varaiya, A.; Kulkarni, M.; Bhalekar,P., and Dogra,J.( 2008). Incidence of metallo-beta-lactamase-producing Pseudomonas aeruginosa in diabetes and cancer patients. Ind.J. pathol.Microbiol. 51:200-203.
Ghazawi, A. A.(2012). Molecular epidemiological studies on sporadic and epidemic isolates of Acinetobacter baumannii Ph.D.Thesis. Biological Doctoral School. University of PÉCS.
Collee, J. G., Fracer, A. G., Marmion, B. P. and Simmon, A. (1996). Practical Medical Microbiology (Mackie and MacCarteny eds). 14th ed. Churchill Livingstone, Singapore.
Sambrook, J.; and Russell, D.(1989). Molecular Cloning: Laboratory Manual. 3rd ed. Cold Spring Harbor, New York.USA.
Bashir,D.; Thokar ,M.A.; Fomda ,B.A.; Bashir , G.; Zahoor ,D.; Shabir Ahmad,A. and Toboli ,A.S. (2011). Detection of metallo- beta- lactamases (MBL) producing Pseudomonas aeruginosa at a tertiary care hospital in Kashmir. Afr. J. Microbiol.Res. .5: 164-172.
Koneman, E.W.; Allen, S.D.; Janda, W.M.; Scheckenber, P.C. and Winn, J.W. (1992). Color Plate and Text Book of Diagnostic Microbiology. 4th ed. J.B. Lippincott company. Washingtion, pp:429.
Backer, F. J. and Silverton , R.E. ( 1985 ) . Introduction of Medical Laboratory Technology, 6th ed. Butterworth and Co. C. Publisher.
Harly, J. and Prescott, L. (2002). Laboratory exercises microbiology. 5th ed. WCB. The Mcgraw- Hill, Companies.New York.
Siau, H.; Yuen, K. ; Ho, P.; Luk, W.; Wong, S. S .Y.; Woo, P. C. X.; Lee, R .A. and Hui, W. (1998). Identification of Acinetobacter on blood agar in presence of D-glucose by inque browing effect. J. Clin. Microbiol. 36(5): 1404-1407.
Forbes, B.A.; Sahm, D.F. and Weissfeld, A.S. (2007). Baily and scott's: Diagnostic microbiology. (12th Edition). Mosby, Inc. Baltimore. USA. P:266-277.
Macfaddin, J. F. (2000). Biochemical tests for identification of medical bacteria. 3rd ed. lippincott Williams and Wilkins.
Coque, T., Patterson J., Steckelberg J. et al. (1995): Incidence of haemolysin, gelatinase and aggregation substance among Enterococci isolated from patients with endocarditis and other infections and from feces of hospitalized and community-based persons. J. Infect Dis; 171: 1223–9.
Holt, J.G.; Kreieg, N.R.; Sneath, P.H.; Staley, J.T. and Williams, S.T.(1994). Bergey`s Manual of Determinative Bcteriology. 9th ed. . Wikins. P:1063.
Clinical and Laboratory Standards Institute (CLSI) (2012). Performance Standards for Antimicrobial Susceptibility Testing; Twenty-second Informational Supplement, 32(3). Wayne, USA.
Sengstock, D.M .;Thyagarajan, R.; Apalara, J.; Mira, A.; Chopra ,T.and Kaye, K.S. (2010). Multidrug-resistant Acinetobacter baumannii: an emerging pathogen among older adults in community hospitals and nursing homes. Clin. Infect. Dis. 50: 1611–1616.
Al-Sehlawi,Z.S.; Almohana,A.M.; Al Thahab,A.A.;(2011). Isolation and Identification of Acinetobacter baumannii Clinical Isolates using Novel Methods. Journal of Babylon University.Pure and Applied Sciences. 3(22):1041_1050.
Al-Muhanna,A. S.J(2006).A molecular and genetic study of virulence factors of Acinetobacter isolated from different infections. Ph.D.Thesis. College of Science .AL- Mustasiriyah University.
Constantiniu, S.; Romaniuc, A.; Iancu, L. S.;Filimon, R., and Taraşi, I.(2004). Cultural and biochemical charactistics of Acinetobacter spp. Strains isolated from hospital units . j. preventive med. . 12 : 35 – 42 .
Lahiri, K. K.; Mani, N.S. and Purai, S.S. (2004). Acinetobacter spp. as Nosocomial Pathogen :Clinical Significance and Antimicrobial Sensitivity . MJAFI . 60(1) :7-10.
Visca, P.; Seifert, H. and Towner, K. J. (2011). Acinetobacter infection, an emerging threat to human health. IUBMB Life. 63(12):1048-1054.
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