Validity of B1 Gene in Diagnosis of Prenatal and Postnatal infection with Toxoplasma gondii
DOI:
https://doi.org/10.36320/ajb/v7.i3.8068Keywords:
B1 Gene , fungicide Bentazole, immunosuppressedAbstract
Toxoplasma gondii is one of the most frequent parasite all over the world. Most patients are asymptomatic, except in immunosuppressed individuals and pregnant women , which can be seriously injured. Prenatal diagnosis should be made rapidly in view of the fact that treatment of the mother can reduce fetal sequelae.
The present study was used Rapid test as a serological method to detected IgG and IgM antibodies titers and the Nested polymerase chain reaction technique (nPCR) in 74 samples of blood were collected from pregnant women whom with history of abortion. The aborted cases were 33 out of 74, while 41cases were reach birth, 15 cases were early birth ( 29-36 week) period and 26 cases were reach birth with normal pregnancy period ( 37-42 week).
The result of Rapid test reveled that IgM and IgG were positive in pregnant women 29/ 74 and 47/74 respectively , while the nested polymerase chain reaction technique (nPCR) result showed 36/74.
The Nested polymerase chain reaction technique (nPCR) was also done on the ( 41) newborn babies. The total number of newborn babies with congenital anomalies were 16 and the positive result of (nPCR) in newborn showed 17. The validity of B1 gene in prenatally toxoplasmosis showed the sensitivity, specificity, positive productive value and negative productive value were (91%, 81% ,78% and 100%) respectively, while the sensitivity, specificity, positive productive value and negative productive value in postnatally were (88%, 83%, 94% and 96%) respectively.
The aim of study was the early finding congenital toxoplasmosis of maternofetal transmission, for early treatment to avoid unwanted sequelae using nPCR technique in support of early diagnosis T. gongii in the prenatal of congenitally and to evaluate these results to the postnatal infection.
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References
Montoya J.G.,. Liesenfeld O.2004. Toxoplasmosis. The Lancet. 363:1965- 1976.
Montoya J.G.,. Liesenfeld O.2004 Toxoplasmosis. The Lancet.; 363:1965-1976. DOI: https://doi.org/10.1016/S0140-6736(04)16412-X
Foulon W, Pinon JM, Stray-Pedersen B, Pollak A, Lapplainen M,
Decoster A, Villena I, Jenum PA, Hayde M, Naessens A.1999. Prenatal diagnosis of congenital toxoplasmosis: a multicenter evaluation of different diagnostic parameters. American Journal of Obstetrics and Gynecology 181:843-847,. DOI: https://doi.org/10.1016/S0002-9378(99)70311-X
Antsaklis A., Daskalakis G., Papantoniou N., Mentis A., Michalas S.2002 Prenatal diagnosis of congenital toxoplasmosis. Prenat Diagn .22: 1107-11.
Pelloux H., Weiss J., Simon J.1996. A new set of primers for the detection of Toxoplasma gondii in amniotic fluid using polymerase chain reaction. FEMS Microbiol Lett .138: 11-5. DOI: https://doi.org/10.1016/0378-1097(96)00057-2
Hohfeld P, Daffos F, Costa J, Thulliez P, Forestier F, Vidaud M.1994. Prenatal diagnosis of congenital toxoplasmosis with a polymerase chain reaction test on amniotic fluid. The New England Journal DOI: https://doi.org/10.1056/NEJM199409153311102
of Medicine 331:695-699
Contini C., Seraceni S., Cultrera R., Incorvaia C., Sebastiani A., Picot S. 2005. Evaluation of a Real-time PCR-based assay using the lightcycler system for detection of Toxoplasma gondii bradyzoite genes in blood specimens from patients with toxoplasmic retinochoroiditis. Int J DOI: https://doi.org/10.1016/j.ijpara.2004.11.016
Parasitol.; 35: 275–283
Jones CD, Okhravi N, Adamson P, Tasker S, Lightman S.2000. Comparison of PCR detection methods for B1, P30, and 18s rDNA genes of T.gondii in aqueous humor. Invest Ophthalmol Vis Sci .41: 634-44
Guy EC, Pelloux H, Lappalainen M, et al.1996. Interlaboratory comparation of polymerase chain reaction for the detection of Toxoplasma gondii DNA added to samples of amniotic fluid. Eur J Clin Micribiol Infect Dis .15: 836-9. DOI: https://doi.org/10.1007/BF01701532
Antsaklis A, Daskalakis G, Papantoniou N, Mentis A, Michalas S.2002. Prenatal diagnosis of congenital toxoplasmosis. Prenat Diagn .22: 1107-11. DOI: https://doi.org/10.1002/pd.476
Sever J.2006. Toxoplasmosis in pregnancy. In Mead P., Hager D., editors. Infection Protocols for Obstetrics and Gynecology. Montvale (NJ): Medical Economics Publishing :131-3.
Calderaro A., Piccolo G., Peruzzi S., Gorrini C., Chezzi C., Dettori G.2008. Evaluation of Toxoplasma gondii Immunoglobulin G (IgG) and IgM Assays Incorporating the New Vidia Analyzer System. Clin. Vaccine Immunol. 15:1076–1079. DOI: https://doi.org/10.1128/CVI.00025-08
Ajzenberg, D., A. L. Ban˜uls, C. Su, A. Dume`tre, M. Demar, B. Carme, and
M. L. Darde´. 2004. Genetic diversity, clonality and sexuality in Toxoplasma
gondii. Int. J. Parasitol. 34:1185–1196.
Burg J.L., Grover C.M., Pouletty P.h., Boothroyd J.C.1989. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. Journal of Clinical Microbiology 27:1787-1792. DOI: https://doi.org/10.1128/jcm.27.8.1787-1792.1989
Colin D. J.,Narciss I. ,Okhravi L., Peter A. ,Tasker S., and Susan L.2000 Comparison of PCR Detection Methods for B1, P30, and 18S rDNA Genes of T. Gondii in Aqueous Humor. IOVS.Vol. 41, No. 3.
Sorlie D.E. (ed). Medical biostatistics and epidemiology: Examination and Board review. First ed, 1995, Norwalk, Connecticut, Appleton and Lange: 47-88.
Montoya J.2002. Laboratory diagnosis of toxoplasmosis gondii infection and toxoplasmosis. J Infect Dis .185(suppl 1):S73-82. DOI: https://doi.org/10.1086/338827
Wilson M., Remington J., Clavet C., Varney G., Pres C., War D., and the FDA Toxoplasmosis Ad Hoc Working Group.1997. Evaluation of six commercial kits for detection of human immunoglobulin M antibodies to Toxoplasma gondii. J Clin Microbiol .35(12): 3112-5. DOI: https://doi.org/10.1128/jcm.35.12.3112-3115.1997
Cristina N., Derouin F., Pelloux H., Pierce R., Cesbron-Delauwn M.F., Ambroise-Thomas P.1992. Détection de Toxoplasma gondii chez des patients sidéens par la technique de “Polimerase Chain Reaction” (PCR), à l’aide de la séquence répétée TGR1E. Pathologie Biologie 40:52-55.
Jenum P.A., Holberg-Petersen M., Melby K.K., Stray-Pedersen B.1998. Diagnosis of congenital Toxoplasma gondii infection by polymerase chain reaction (PCR) on amniotic fluid samples. Acta Pathologica, Microbiologica, et Immunologica Scandinava .106:680-686. DOI: https://doi.org/10.1111/j.1699-0463.1998.tb00211.x
Elisabeth C., Laurence I., Lachaud L.C.and Patrick B. 2004.Comparison of Two Widely Used PCR Primer Systems for Detection of Toxoplasma in Amniotic Fluid, Blood and Tissues. J. Clin.Micro. 42:1719–1722. DOI: https://doi.org/10.1128/JCM.42.4.1719-1722.2004
Dutton G.N., McMenamin P.G., Hay J., Cameron S.1986. The ultrastructural pathology of congenital murine toxoplasmic retinochoroiditis, II: the morphology of the inflammatory changes. Exp Eye Res. 43:545–560. DOI: https://doi.org/10.1016/S0014-4835(86)80022-7
Calderaro A., Peruzzi P., Piccolo G., Gorrini C., Montecchini S., Rossi S., Chezzi C., Dettori G.2009. Laboratory diagnosis of Toxoplasma gondii infection. Int. J. Med. Sci. 6:123-125. DOI: https://doi.org/10.7150/ijms.6.135
Paula V. T.V., Flلvia C. C., Ricardo W. de and Geraldo B. Fi. 2002.Prenatal toxoplasmosis diagnosis from amniotic fluid by PCR. Revista da Sociedade Brasileira de Medicina Tropical 35: 1-6. DOI: https://doi.org/10.1590/S0037-86822002000100001
Jitbanjong T. W., Rachatawan C. and Yaowalark S.2012. PCR Techneque for Detection Toxoplasma gondii in Animal Amoniotic Fluid. South. Asia.J. Trop.Health.. 35: 4.
Desmonts G 1996. Definitive serological diagnosis of ocular toxoplasmosis.
Arch Ophthalmol. 76:839–851.
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