Assessment of Viral load for Human Cytomegalovirus in Patients with Hepatitis B Virus
DOI:
https://doi.org/10.36320/ajb/v7.i3.8074Keywords:
Cytomegalovirus, Hepatitis B Virus, PCRAbstract
This study was carried out on Seventy-Six samples, formed (55 males and 21 females) with age ranging (11-72) year were collected from center Health Laboratory/Al-Hakeem Hospital, and AL-Sadder medical city in AL-Najaf city, during the period from January (2013) to August (2013). Fifteen healthy individuals without any evidence of chronic inflammatory disease involved as control, age ranging (21-50) years. All patients were divided in six age groups. Blood samples were collected from patients and control for immunological (IgM, IgG & TFN-α) by using ELISA and molecular study by RT-PCR, respectively. The results showed that 76 HBsAg seropositive in all age groups but the age group (44-54) year revealed high significant(p<0.05) than other age groups and male more infection than female. The ELISA test results showed that 68 (89.4%) out of the 76 samples were positive for anti-HCMV IgG antibodies, and 4(5.26%) samples were positive for anti-HCMV IgM. The results of the Real-Time PCR revealed that HCMV DNA were detected in 23(30.2%) out of 76 patients were found in all age groups with viral loads ranging from (0.24- 1730000) Copies/ml, and the results of controls group in Real-Time PCR were HCMV negative. The results of cytokines profile (TNF-α) showed a highly significant (P<0.05) elevation in the serum of all patients than control groups.
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Abdul-Husin, I.F. (2013). Epidemiological Study of Viral Hepatitis Types B and C in Babylon Province. M.Sc. thesis. College of Medicine .University of Babylon.1-114.
Alexander, I. K. and Kourtis, A.P. (2007).Hepatitis B Updated. American Journal of Gastroenterology; 201(3): 297-298.
Ali, E.A. (2012). Detection of Human Cytomegalovirus in pregnant women Using Quantitative Real-Time PCR. MSc. thesis .College of Science. University of Baghdad, Iraq.
Al-Jadiry, M. F.; Al-Khafagi M.; Al-Darraji A.F.; Al-Saeed R.M.; Al-Badri S.F. and Al-Hadad S.A. (2013). High incidence of hepatitis B infection after treatment for pediatric cancer at a teaching hospital in Baghdad. Eastern Mediterranean Health Journal (EMHJ). 19 (2):130-134. DOI: https://doi.org/10.26719/2013.19.2.130
Al-Khozai, Z. M.F. (2006). Study of Epidemiological and Immunological aspects on Viral Hepatitis type B and C in Al-Qadisiyia Province. Ph.D. Thesis. College of Science. University of Baghdad.1-122.
Al-Rawi, K.M.and Khalaf Allah, A.M. (2000). Design and Analysis of Agricultural Experiments. Dar Al kutob for printing and publishing. Al-Mousil University. Iraq (In Arabic).
AL-Saidi, A.A. (2012). Molecular Genotypes and Immunological study on patients with Hepatitis B Virus in Middle Euphrates of Iraq.Ph.D.Thesis.Colleg of Medicine/Kufa University. Iraq. 1-120.
Andrew, A.; Henry, B.; Foster, G.; Isaac, B.; Clement, A. and Isaac, A. (2008). Seroprevalence of HHV-8, CMV, and EBV among the general population in Ghana, West Africa. BMC Infectious Diseases. Doi: 10.1186/1471-2334-8-11. DOI: https://doi.org/10.1186/1471-2334-8-111
Awan, Z.; Idrees M.; Amin I.; Butt S.; Afzal S.; Akbar H.; Rehman I.; Younas S.; Shahid M.; Lal A.; Saleem S. and Rauff B.(2010). Pattern and molecular epidemiology of Hepatitis B virus genotypes circulating in Pakistan. Infection, Genetics and Evolution; 10:1242-1246. DOI: https://doi.org/10.1016/j.meegid.2010.08.006
Baig, S. (2009). Gender Disparity in infections of Hepatitis B virus. Journal of the College of Physicians and Pakistan; 19(9):598-600.
Barzilai O.; Sherer Y.; Ram, M.; Izhaky D.; Anaya J.M. and Shoenfeld, Y. (2007).Epstein–Barr Virus and Cytomegalovirus in Autoimmune Diseases Are They Truly Notorious? A Preliminary Report. Ann. N.Y. Acad. Sci. 1108: 567–577. DOI: https://doi.org/10.1196/annals.1422.059
Binnicker M. J. and Espy M. E. (2013). Comparison of Six Real-Time PCR Assays for Qualitative Detection of Cytomegalovirus in Clinical Specimens. J. Clin. Microbiol; 51(11):3749. DOI: https://doi.org/10.1128/JCM.02005-13
Blumberg, B.S., (2006). The curiosities of hepatitis B virus: prevention, sex ratio and demography. Proceedings of the American Thoracic Society. DOI: https://doi.org/10.1513/pats.200510-108JH
Brown, E.G.; Dooley D.S. and Chapman R.(2013). Chronic Hepatitis B and Hepatitis C Infections in California: Cases Newly Reported through 2011. California Department of Public Health (CDPH).
David, C. D. and Daniel, D. F., (2003). Scientific American medicine. Web MD Inc.USA; 824.
Daw, M. A. and El-Bouzedi A. (2014). In association with Libyan Study Group of Hepatitis & HIV Prevalence of hepatitis B and hepatitis C infection in Libya: results from a national population based survey. BMC Infectious Diseases; 14(17):1-9. DOI: https://doi.org/10.1186/1471-2334-14-17
Daw, M.A.; Siala I.M.; Warfalli M.M and Muftah M.I. (2000). Seroepidemiology of hepatitis B Virus markers among hospital health Care Workers. Saudi Medical Journal.21 (12):1157-1160.
Drew, W.L. (2011). Cytomegalovirus. In: Goldman L, Schafer AI, eds. Cecil Medicine. 24th ed. Philadelphia, Pa: Saunders Elsevier; chap 384.
Hassan, H. M. M.; Alsamarai A. M.; M.Aljumaili Z. K. and Alobaidi A. H. (2014). Association between Cytomegalovirus Infection and Bad Obstetric Outcomes in Women from Kirkuk. International Journal of Public Health Science (IJPHS); 3(1): 29-42. DOI: https://doi.org/10.11591/ijphs.v3i1.4672
Hsu T. (2014). Human Cytomegalovirus and Autoimmune Disease. Hindawi Publishing Corporation. Biomed Research International. Article ID 472978, 15 pages. DOI: https://doi.org/10.1155/2014/472978
Jahan, M. (2010) Laboratory Diagnosis of CMV Infection: A Review. J Med Microbiol; 04 (02): 39-44. DOI: https://doi.org/10.3329/bjmm.v4i2.10831
Khan, F.; Shams S.; Qureshi I. D.; Israr M.; Khan H.; Sarwar M. T. and Ilyas M. (2011). Hepatitis B virus infection among different sex and age groups in Pakistani Punjab. Virology Journal; 8:220-225. DOI: https://doi.org/10.1186/1743-422X-8-225
Kursad, M.; Hamza B.; Ismail U. and Ilyas T. (2005). Chronic hepatitis delta virus infection in Van region of eastern Turkey. The Turkish Journal of Gastroenterology. 16(1).17-20.
Lewis, S. M.; Bgin B. J. and Bates A. (2006). Daci and Lewis practical hematology. 10th ed. Churchill Livingston. USA. 1-5.
Lindenberg, A.S.; Motta-Castro A.R.; Puga M. A.; Tanaka T. S.; Torres M. S.; Fernandes-Fitts S.M. and Cunha R. V. (2013). Decrease in hepatitis B prevalence among blood donors in Central-West Brazil. Journal of Venomous Animals and Toxins including Tropical Diseases; 19:7.1-3. DOI: https://doi.org/10.1186/1678-9199-19-7
Liu, Z.; Zhang P.; Tang S.; He X.; Zhang R.; Wang X.; Yuan Zh.; Tan J.; Liu E.; Fu Zh. and Liu L. Z.. (2014). Urine real-time polymerase chain reaction detection for children virus pneumonia with acute human cytomegalovirus infection BMC Infectious Diseases, 14:245.1-9. DOI: https://doi.org/10.1186/1471-2334-14-245
Machado, D.F.; Martins T.; Trevisol D.; Silva R.A.; Narciso-Schiavon J.; Trevisol F. and Schiavon L. (2013). Prevalence and Factors Associated with Hepatitis B Virus Infection Among Senior Citizens in a Southern Brazilian City. Hepat J; 13(5): 7874-7884. DOI: https://doi.org/10.5812/hepatmon.7874
Masoodi, T., Sardana R., Hassan A., Jasuja S., Aggarwal D.K., Dawar R., Mufti,G.N., Mendiratta L. and Masoodi K.Z. (2013). A study on the Timeline of Onset of Opportunistic Cytomegalovirus. International Journal of Molecular Medical Science; 3(6):41-49.
Mehta, K.; Garala N.; Garala R.; Hansaliya M.; Shah A.; Aring B. and Sinha M. (2014).The study of prevalence of Hepatitis B surface antigen during pregnancy. J Res Med Den Sci; 2(2):17-19. DOI: https://doi.org/10.5455/jrmds.2014224
Of Gastroenterology; 201(3): 297-298.
Paixão, P.; Almeida S.; Videira P.A.; Ligeiro D. and Marques T. (2012). Screening of congenital cytomegalovirus infection by real-time PCR in urine pools. Eur J Pediatr, 171(1):125–129. DOI: https://doi.org/10.1007/s00431-011-1496-4
Park, Y.; Jun Y. P.; Kwang-Hyub H. and Hyon-Suk K. (2012). Serum cytokine levels in chronic hepatitis B patients receiving peginterferon alpha-2a therapy. Hepatobiliary Pancreat Dis In; 11:499-506. DOI: https://doi.org/10.1016/S1499-3872(12)60214-8
Qureshi, H., Arif A., Riaz K., Alam E.S., Ahmed W. And Mujeeb A.S. (2009). Determination of risk factors for hepatitis B and C in male patients suffering from chronic hepatitis. BMC Research Notes, 2:212. DOI: https://doi.org/10.1186/1756-0500-2-212
Rehermann, B. (2000). Intrahepatic T Cells in Hepatitis B: Viral Control versus Liver Cell Injury. The Journal of Experimental Medicine; 191(8):1263-1268. Review in a Canadian Pediatric Hospital. ISRN Pediatrics. ISSN: 2090469. DOI: https://doi.org/10.1084/jem.191.8.1263
Salih,G.F.(2014).Identification and Genotyping of Hepatitis B Virus by PCR assay using Genotype Specific Primers .European Scientific Journal.10(9):1857-7881.
Tokushige, K.; Yamaguchi N.; Ikeda; Hashimoto E.; Yamauchi K. and Hayashi, N. (2000). Significance of soluble TNF receptor-I in acute type fulminant hepatitis. Am.J.Gastroenterol. 95: 2040-2046 DOI: https://doi.org/10.1111/j.1572-0241.2000.02270.x
Vries, J.J.; Annemiek A.E.; Katja C.W.; Lisette G.R.; Suzan D.P.; Richard M; Eric C.C.; Aloys C.M.; and Ann C.T. (2012). Real-time PCR versus viral culture on urine as a gold standard in the diagnosis of congenital cytomegalovirus infection. J.Clin. Virol. 53:167-170. DOI: https://doi.org/10.1016/j.jcv.2011.11.006
Wreghitt, T.G.; Teare E.L.; Sule O.; Dev R. and Rice. (2003). Cytomegalovirus Infection in Immunocompetent patients. Clinical Infectious Diseases; 37:1603-1606. DOI: https://doi.org/10.1086/379711
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Copyright (c) 2016 Huda J.B.AL- Khilkhali, Mohammed A. Muhsin, Mohammed A. K. AL-Saadi
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