Antibiotics susceptibility of E.cloacae and E.sakazakii that isolated from different clinical specimens
DOI:
https://doi.org/10.36320/ajb/v7.i1.8090Keywords:
E.cloacae , E.sakazakii , Antibiotics susceptibilityAbstract
Two species of Enterobacter (84 isolates) E.cloacae (75 isolates) and E.sakazakii (9 isolates) were isolated from different clinical specimens .
They are observed , all isolates (84) were resistant to β– lactam antibiotics (amoxicillin and cefoxitin) but the resistance for cephalothin ,cephalexin and ampicillin , were 98% , 92% and 90% respectively , 75% , 61% and 50% for doxycycline , nitrofurantion and chloramphenicol respectively .The high values of MICs were registered to cefoxitin (46-256) μg / ml , while the lower MICs values of cefepime , imipenem and ciprofloxacin were (0.5 -32 , 0.5 -16 , 0.5 – 4) μg / ml respectively .
From the study 72 (86%) of isolates were produced β– lactamase , 63 isolates (84%) of E.cloacae can produce β –lactamase , and all isolates (9)of E.sakazakii gave positive result for β –lactamase.
Downloads
References
A bid , I. N. ( 2006 ) . Ecological and bacteriological study of contaminated drinking water with indicator bacteria and its sensitivity to antibiotics in Nassiriyah city. M.SC. Thesis. college of Education , Thi-Qar University . Iraq.
Astal , Z. E. ( 2005 ). Increasing ciprofloxacin resistance among prevalent urinary tract bacterial isolates in the Gaza strip . J. Chemother . 46 (9) : 457 – 460 . DOI: https://doi.org/10.1155/JBB.2005.238
Bradford , P. A. ( 2001 ) . Extended – Spectrum – beta – lactamases in the 21st century : Characterization , epidemiology , and detection of this important resistance threat . Clinical microbiology Reviews , 14 : 933 – 951 . DOI: https://doi.org/10.1128/CMR.14.4.933-951.2001
Bradford , P. A. ; Urban , C. ; Mariano , N. ; Projan , S. J. ; Rahal , J. J. and Bush, K. (1996). Imipenem resistancein Klebsiella pneumoniae is associated with the combination of ACT – 1, a plasmid – mediated Amp C β – lactamase , and the loss of outer membrane protein . Antimicrobial . Agents and chemother . , P : 563 – 569 DOI: https://doi.org/10.1128/AAC.41.3.563
Brooks , G. F. ; Butel , J. S.; Carroll,K.C. and Morse , S. A. ( 2007 ) . Jawetz , Melnick & Alderge's Medical microbiology . 24th ed. McGraw-Hill . Companies . Inc. , PP: 161 – 195 .
Bush , K. ; Jacoby , G. A. and Medeiros , A. A. ( 1995 ) . A functional classification sheme for β -lactamases and its correlation with molecular structures . Antimicrob . Agents Chemother . , 39 : 1211 – 1233 . DOI: https://doi.org/10.1128/AAC.39.6.1211
Chow , J. W. ; Fine , M. J. ; Shales , D. M. ; Quinn , J. P. ; Hooper , D. C. ; Johnson , M. P. ; Ramphal , R. ; Wagener , M. M. ; Miyashiro , D. K. and Yu , V. L. ( 1991 ) . Enterobacter bacteremia : Clinical features and emergence of antibiotic resistance during therapy . Ann . Intern . Med. 115 : 585 – 590. DOI: https://doi.org/10.7326/0003-4819-115-8-585
Clinical and Laboratory standards Institute ( CLSI ) . (2006) . Performance standards for antimicrobial susceptibility testing . 16th ed . Informational supplement , Wayne , M. 100 – 216 .
Clinical and Laboaratory standards Institute (CLSI ) . ( 2007 ) . Performance standards for antimicrobial susceptibility testing seventeenth informational supplement M 100 – S17 . Clinical and Laboratory Standards Institute, Wayne , USA .
Green wood , D. ; Slack , R., ; Peutherer , J. and Barer , M. ( 2007 ) . Medical Microbiology 7th ed . Churchill , Livingstone , Elsevier . P : 738 .
Harvey , R. A. and Champe , P. C. (2006) . Lippincott's Illustrated Reviews : Pharmacology . 3rd ed . Wolters Kluwer Company , USA , pp . 386 .
Harwood ,V . J. ; Whitlock , J. and Withington , V. ( 2000 ) . Classification of antibiotic resistance patterns of indicator bacteria by discriminate analysis : use in predicting the source of fecal contamination in subtropical waters . App. Environ . for Microbiol . , P: 3698 – 3704 . DOI: https://doi.org/10.1128/AEM.66.9.3698-3704.2000
Karlowsky , J. A. ; Jones , M. E. ; Thornsberry , C. ; Friedland , I.R. ; Sahm , D. F. ( 2003 ) . Trends in antimicrobial susceptibilities among Enterobacteriaceae isolated from hospitalized patients in the united states from 1998 to 2001 . Antimicrob Agents chemother . ; 47 : 1672 – 80 . DOI: https://doi.org/10.1128/AAC.47.5.1672-1680.2003
Kesseler , R. E. ; Bies , M. ; Buck , R. E. ; Chisholm , D. R. ; Pursinao , T. A.; Tsai , Y. H. ; Misiek , M.; Price , K. E. and Leitner , F. (1985) . Comparsion of a new cephalosporin , BMY 28142 , with other broad -spectrum β -lactam antibiotics . Antimicrob -Agents Chemother . , 27 : 207 – 216 . DOI: https://doi.org/10.1128/AAC.27.2.207
Kolar , M . ; Bardon , J. Chroma , M. ; Hricova , K. ; Stosova , T. ; Sauer , P. and Koakalova , D. ( 2010 ) . ESBL and Amp C beta-lactamase – producing Enteriobacteriaceae in poultry in the Czech Republic . Veterinary Med. , 55 (3) : 119 – 124 . DOI: https://doi.org/10.17221/165/2009-VETMED
Koneman, E. W.; Allen, S. D.; Janda , W. M.; Scheckenber, P. C. and Winn, J. W. (1992). Color plate and text book of diagnostic microbiology. 4th ed. J. B. Lippincott Company Washington, PP. 429.
Kunapuli , T. L. ; Madhusudhan , C. C. ; Lody , C. ; Carter , O. ; Dodson , S. and Ojha , N. ( 2003 ) . Comparative in vitro Activity of three flouroquinolones against clinical isolates by E test. Chemother . 49 (4 ) : 184 – 188 . DOI: https://doi.org/10.1159/000071142
Laurence, D. R. ; Bennett , P. N. and Brown , M. J. ( 1997 ) . Antimicrobial drugs in: Clinical pharmacology. 8th ed. Churchill Livingston, London.
Livermore, D.M. (1995). β -lactamases in Laboratory and clinical resistance. Clin. Microbiol Rev. 8 : 557 – 584 . DOI: https://doi.org/10.1128/CMR.8.4.557
Lngram , P. R. ; Lnglis , T. J. J. ; Vanzetti , T. R. ; Henderson , B. A. ; Harnett , G. B. and Murray , R. J. ( 2011 ) . Comparison of Methods for Amp C – β -lactamase detection in Enterobacteriaceae . Journal of Med. Microbiol . 60 : 715 – 721 . DOI: https://doi.org/10.1099/jmm.0.029140-0
Maes , P. and Vanhoof , R. ( 1992 ) . A. 56 – Month prospective surveillance study on the epidemiology of aminoglycoside resistance in a Belgian general hospital . Scand – J. Infect . Dis. 24 – 495 – 501 . DOI: https://doi.org/10.3109/00365549209052636
Manchanda , V. and Singh , N. P. ( 2003 ) . Occurrence and detection of Amp C β -lactamases among gram-negative clinical isolates using a modified three – dimensional test at Guru Tegh Bahadur Hospital , Delhi , India . J. Antimicrob . Chemother ., 51 : 415 – 8 . DOI: https://doi.org/10.1093/jac/dkg098
Martinez , J. L. ; Parscual , A. and Jacoby , G. A. ( 1998 ) . Quinolone resistance from a transferable plasmid . The lancet . , 35 (4) : 797 – 799 . DOI: https://doi.org/10.1016/S0140-6736(97)07322-4
Mayer, K. H.; Fling, M. E. ; Hopkins , J. D. and O' Brein , T. F. ( 1985 ). Trimethoprim resistance in multiple genera of Enterobacteriaceae as a U.S. hospital: spread of the type II dihydrofolate reductase gene by a single plasmid. Journal of infectious diseases, Vol. 151 number 5. DOI: https://doi.org/10.1093/infdis/151.5.783
Murray, I. and Show, W. V. (1997). Acetyl transferase for chloramphenicol and other natural products. Antimicrobio – Agents chemother . , 41 (1) : 1 – 6 . DOI: https://doi.org/10.1128/AAC.41.1.1
National Committee for Clinical Laboratory Standards ( NCCLS).(2003). Performance standards for disc susceptibility tests. 8th ed. Approved standards M2-A8.National Committee for Clinical Laboratory Standards, Wayne, Pa.
Nordmann , P. and Poirel , L. ( 2002 ) . Emerging carbapenemase in gram-negative aerobes . clin. Microbiol . Infect. 8 : 321 – 331 . DOI: https://doi.org/10.1046/j.1469-0691.2002.00401.x
Park , Y. J. ; Lee , S. ; Park , J. J. ; Park , K. G. ; Kim , B. K. and Kang , C. S. (2003) . Antimicrobial susceptibility of inducible Amp C β -lactamases - producing Enterobacter cloacae , citrobacter freundii and Serratia marcescens . Korean survey Department of clinical pathology . , 51 (4) : 265 – 9 .
Paterson , D. L. ( 2006 ) . Resistance in gram negative bacteria : Entrobacteriaceae . Am . J. Med. , 119 ( 6 ) : 62 – 70 . DOI: https://doi.org/10.1016/j.amjmed.2006.03.013
Paterson, D. L. and Bonomo, R. A. (2005) . Extended spectrum beta – lactamases : a clinical update.clinical microbiology reviews , 18 : 657-686. DOI: https://doi.org/10.1128/CMR.18.4.657-686.2005
Piddock , L. J. V. ( 1990 ) . Techniques used for the determination of antimicrobial resistance and sensitivity in bacteria . J. Appl . Bacteriol . 968 : 307 – 318 . DOI: https://doi.org/10.1111/j.1365-2672.1990.tb02880.x
Pitout , J. D. ; Moland , E. S. ; Sanders , C. C. ; Thomson , K. S. and Fitzsimmons , S. R. (1997 ) . Beta-lactamases and detection of beta-lactam resistance in Enterobacter Spp. Ant. Agents chemother . 41 (1) : 35 – 39 . DOI: https://doi.org/10.1128/AAC.41.1.35
Poole , K. ( 2004 ) . Resistance to β–lactam antibiotics . cell. Mol. Life . Sci , 61 : 2200 – 2223 . DOI: https://doi.org/10.1007/s00018-004-4060-9
Salman , H. D. (2006) . A bacteriological study of local strains of Enterobacter cloacae isolated from urine samples .M.Sc. Thesis, college of Medicine, University of Babylon, Iraq.
Sanders , W. E. ; Terney , T. H. and Kessler , R. E. (1996) . Efficacy of cefepime in the treatment of infections due to multiply resistant Enterobacter species . Clin. Infect. Dis., 23 : 454 – 461 . DOI: https://doi.org/10.1093/clinids/23.3.454
Stock, E.J. and Ridgway , G. L. (1987). Hardting clinical specimens for microbiological studies . 5th ed. Churchill livingstone . Edinburgh
Tumbarello , M. ; Spanu , T. ; Sanguinetti , M. ; Citton , R.; Montouri , E.; Lcone , F. ; Fadda , G. and Cauda , R. (2006) . Bloodstreem infections caused by extended spectrum – β -lactamases – producing Klebsiella pneumoniae : risk factors, molecular epidemiology and clinical outcome , Antimicrob . Agents Chemother . 50 : 498 – 504 . DOI: https://doi.org/10.1128/AAC.50.2.498-504.2006
Tzouvelekis , L. S. ; Tzelepi , E. and Tassios , P. T. ( 2000 ) . CTX-M-type β -lactamases: an emerging group of extended spectrum enzymes . Int. J. Antimicrob. Agents . 14 : 137 – 142 . DOI: https://doi.org/10.1016/S0924-8579(99)00165-X
Vatopoulos , A. C. ; Philippon , A. ; Tzouvelekis , L.S. ; Komninou , Z. and Legakis , N. J. ( 1990 ) . Prevalence of a transferable SHV – 5 type β -lactamases in clinical isolates of Klebsiella pneumoniae and Escherichia coli in Greece. J. Antimicrob. Chemother. 26: 635-648. DOI: https://doi.org/10.1093/jac/26.5.635
Wang , F. U. (2002) . Antimicrobial mechanism of drug action and bacterial resistance. Yumer , W. ( eds. ) Fine compilation of modern microbiology . Shanghai : Fudan University publishing , P : 306 .
Yigit , H. ; Anderson, G. J. ; Biddle, J. W.; Stewards, C.D. ; Rasheed , J. K. ; Valera, L.L., McGowan,J. E. and Tenover, F.C. (2002). Carbapenem resistance in a clinical isolate of Enterobacter aerogenes in associated with decreased expression of OMPF and OMPC porin analogs . Antimicrob . Agents chemother . 46 : 3817 – 3822 . DOI: https://doi.org/10.1128/AAC.46.12.3817-3822.2002
Yu , W. L. ; Cheng , K. C. ; Chi , C. J. ; Chen , H. E. ; Chuang , Y. C. and Wu , L. T. ( 2006 ) . Characterization and molecular epidemiology of extended-spectrum beta-lactamase producing Enteriobacter cloacae isolated from a district teaching hospital in Taiwan . Clin. Microbiol Infect , 12 : 579 – 82 . DOI: https://doi.org/10.1111/j.1469-0691.2006.01384.x
Zhihui , Z. ; Lanjuan , L. ; Yunsong , Y. and Yilin , M. ( 2003 ) . The status of drug resistance and ampC gene expression in Enterobacter cloacae , Chinese medical Journal , 116(8) : 1244 – 1247 .
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2016 Intidhaar Na,eem Abid, Mahdi H. Al-Amaar
This work is licensed under a Creative Commons Attribution 4.0 International License.
which allows users to copy, create extracts, abstracts, and new works from the Article, alter and revise the Article, and make commercial use of the Article (including reuse and/or resale of the Article by commercial entities), provided the user gives appropriate credit (with a link to the formal publication through the relevant DOI), provides a link to the license, indicates if changes were made and the licensor is not represented as endorsing the use made of the work.