Bioactivity of Bacteria Bacillus subtilis and Pseudomonas fluorescens against Rhizoctonia solani and Fusarium solani, which causes seed rot, seedling on cucumber in greenhouses
DOI:
https://doi.org/10.36320/ajb/v10.i3.8107Keywords:
Rhizoctonia solani, Fusarium solani, Bacillus subtilis, Pseudomonas fluorescensAbstract
The research contracts with the study of the antagonistic effect of microbe against cucumber disease caused by Rhizoctonia solani and Fusarium solani. --Bacillus subtilis and Pseudomonas fluorescens were isolated from cucumber field soil. Some of the fungi diagnosing that cause the death of cucumber seeds in the greenhouse in Najaf Governorate. The results showed identification of R. solani and F. solani was carried out using the PCR technique,with forefronts and back-fronts primers in a range of about 600 pairs to a nitrogen base (Base paris,bp). The analysis showed a new isolation of Rhizoctonia solani in the world according to NCBI. The bacteria isolated from the greenhousein some areas of Najaf Governorate.Results showed that Bacillus subtilis and Pseudomonas fluorescens were used as antifungal agents. Both of them have high resistance against R. solani and F. solani. Both bacteria P. fluorescens and B. subtilis have inhibited the growth of pathogenic fungus by 71.41% especially dilution 1-10..the concentration 20, 25% of bacteria B. subtilis showed 100% inhibiting the growth of R.solani. As well as the concentration 25% of P. fluorescens showed 100% inhibiting the growth of F. solani.
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