Determination of the Role of T-Helper 17 and 22 vs T-reg Cells in Patients with Molluscum Contagiosum
DOI:
https://doi.org/10.36320/ajb/v10.i3.8115Keywords:
Molluscum Contagiosum, T Helper 17, T Helper 22, Regulatory T CellAbstract
Molluscum contagiosum is skin disease and well known by dermatologists, the causative agent is a pox virus. Infection, usually self-limited within few months in individuals with a normal health and immune system. From 205 case females recorded 95(46.3%) and 110(53.7%) males. The results showed that there were a highly significant increment in concentration of IL-17A (132.66 ± 24.7pg/ml) compared with control group which was (42.59±13.4pg/ml). Results also showed that there is highly significant increment in concentrations of IL-23P19 (112.33 ±17pg/ml) compared with control group which was (21.11±9.6pg/ml). Also documented data declared a highly significant increment in concentrations of IL-22 (77.11±8.8pg/ml) in comparison to control group which was (18.11±12pg/ml). The relative expression of the gene FoxP3 gene showed increment in patients group (17.723 ± 1.227) compared with control group which was (2.133±1.234), results of NFAT gene showed increment in patients group (13.676±2.223) compared with control group which was (2.743±1.133) and result of AP1gene showed increment in patients group (3.7883±1.234) compared with control group which was (1.8766±0.889). These findings indicate the potent immune response against the virus infection which belongs to Th-17 and 22 and the controlling mechanisms through the regulatory T-cells which maintain the response in the normal levels.
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Chen X, Anstey AV, Bugert JJ (2013). Molluscum contagiosum virus infection. Lancet Infect Dis 13: 877-888.
Ordoukhanian E. (1997). Warts and molluscum contagiosum: beware of treatments worse than the disease. Postgraduate Medicine; 101(2): 223–32.
Van der Wouden JC, van der Sande R, van Suijlekom-Smit LW, Berger M, Butler C.C.(2009) Interventions for cutaneous molluscum contagiosum. Cochrane Database Syst Rev: 4.
Zhu J, Yamane H, Paul WE. (2010) Differentiation of Effector CD4 T Cell Populations. Annu Rev Immunol; 28: 445–489.
Annunziato F, Cosmi L, Romagnani S. (2010) Human and murine Th17. Curr Opin HIV AIDS; 5: 114–119.
Mosmann TR, Coffman RL. (1989). TH1 and TH2 cells: different patterns of lymphokine secretion lead to different functional properties. Annu Rev Immunol.; 7: 145-73.
Vogelzang A, McGuire HM, Yu D, Sprent J, Mackay CR, King C. (2008). A fundamental role for interleukin-21 in the generation of T follicular helper cells. Immunity.; 29(1): 127-37.
Ramirez JM, Brembilla NC, Sorg O, Chicheportiche R, Matthes T, Dayer JM, Saurat JH, Roosnek E, Chizzolini C. (2010 ) Activation of the aryl hydrocarbon receptor reveals distinct requirements for IL-22 and IL-17 production by human T helper cells. Eur J Immunol.; 40(9): 2450-9.
Ma CS, Tangye SG, Deenick EK. (2010). Human Th9 cells: inflammatory cytokines modulate IL-9 production through the induction of IL-21. Immunol Cell Biol.; 88(6): 621-3.
Harrington L. E., Hatton R. D., Mangan P. R., Turner H., Murphy T. L., Murphy K. M. and Weaver C. T. (2005).
Interleukin 17-producing CD4 effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages. Nat Immunol, 6: 1123-1132.
Leipe J, Grunke M, Dechant C, Reindl C, Kerzendorf U, Schulze-Koops H, (2010). Role of Th17 cells in human autoimmune arthritis. Arthritis Rheum.; 62: 2876–85.
Zhao X, Zhu D, Ye J, Li X, Wang Z, Zhang L, Xu W. (2015). The potential protective role of the combination of IL-22 and TNF-alpha against genital tract Chlamydia trachomatis infection. Cytokine. 73(1): 66–73.
Trifari S, Kaplan CD, Tran EH, Crellin NK, Spits H. (2009). Identification of a human helper T cell population that has abundant production of interleukin 22 and is distinct from T(H)-17, T(H)1 and T(H)2 cells. Nat Immunol; 10(8): 864-71.
Larsen M, Arnaud L, Hie M, Parizot C, Dorgham K, Shoukry M, (2011). Multiparameter grouping delineates heterogeneous populations of human IL-17 and/or IL-22 T-cell producers that share antigen specificities with other T-cell subsets. Eur J Immunol; 41(9): 2596-605.
Rudensky AY. (2011). Regulatory T cells and Foxp3. Immunol Rev.; 241(1): 260-8.
Kretschmer, K., I. Apostolou, D. Hawiger, K. Khazaie, M. C. Nussenzweig, and H. von Boehmer. (2005). Inducing and expanding regulatory T cell populations by foreign antigen. Nat Immunol 6: 1219–1227.
Wu Y, Borde M, Heissmeyer V, Feuerer M, Lapan AD, Stroud JC, Bates DL, Guo L, Han A, Ziegler SF, Mathis D, Benoist C, Chen L, Rao A. (2006). FOXP3 controls regulatory T cell function through cooperation with NFAT. Cell.; 126(2): 375-387.
Macian F (2005) NFAT proteins: key regulators of T-cell development and function. Nat Rev Immunol; 5: 472–484.
Rao, A., Luo, C., and Hogan, P. G. (1997). Transcription factors of the NFAT family: regulation and function. Annu. Rev. Immunol. 15, 707–747.
Hayase H, Ishizu A, Ikeda H, Miyatake Y, Baba T, Higuchi M, (2005). Aberrant gene expression by CD25+CD4+ immunoregulatory T cells in autoimmune-prone rats carrying the human T cell leukemia virus type-I gene. The Japanese Society for Immunology 17: 677–684.
Wang, G. and Hardy, M. P. (2004). Development of lending cells in the insulin-like growth factor-I (igf-I) knockout mouse: effects of igf-I replacement and gonadotropic stimulation. Biol Reprod. 70: 632–639.
Radonic A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A (2004) Guideline to reference gene selection for quantitative real-time PCR. Biochem Biophys Res Commun, 313(4): 856–862.
McDonald, J. H. (2009). Handbook of Biological Statistics. 2nd ed., Sparky House Publishing, Baltimore, Maryland.
Rogers M, Barnetson RSC. Diseases of the skin. In: Campbell AGM, McIntosh N (2013). Forfar and Arneil’s Textbook of Pediatrics. 5th Edition. New York: Churchill Livingstone, 1998: 1633–5.
Zheng S. G. (2013). Regulatory T cells vs Th17: differentiation of Th17 versus Treg, are the mutually exclusiveAm. J Clin Exp Immunol; 2(1): 94-106.
Dong C. (2008). TH17 cells in development: an updated view of their molecular identity and genetic programming. Nat Rev Immunol, 8: 337e348.
Cua DJ. &Tato CM. (2010). Innate IL-17-producing cells: the sentinels of the immune system. Nat. Rev. Immunol. 10, 479-489.
Takayanagi H. (2012). New developments in osteoimmunology. Nat Rev Rheumatol.; 8(11): 684-9.
Mirshafiey A, Simhag A, El Rouby NM, Azizi G. (2015) T-helper 22 cells as a new player in chronic inflammatory skin disorders. Int J Dermatol.; 54(8): 880-8.
Sabat R., Ouyang W. & Wolk K. (2014) Therapeutic opportunities of the IL-22–IL-22R1 system. Nature Reviews Drug Discovery 13, 21–38.
Tone M, Greene MI. (2011) Cooperative regulatory events and Foxp3 expression. Nat. Immunol., 12(1): 14-6, 2011.
Williams, L. M. and Rudensky, A. Y. (2007). Maintenance of the Foxp3-dependent developmental program in mature regulatory T cells requires continued expression of Foxp3. Nat. Immunol.; 8: 277–284.
Ruland J, Mak TW. (2003) Transducing signals from antigen receptors to nuclear factor kappaB. Immunol Rev.; 193: 93–100.
Lorentz A, Klopp I, Gebhardt T, Manns MP, Bischoff SC. (2003). Role of activator protein 1, nuclear factor-kappaB, and nuclear factor of activated T cells in IgE receptor-mediated cytokine expression in mature human mast cells. J Allergy Clin Immunol.; 111: 1062–8.
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