Antioxidant role of Lactobacillus sp isolated from honey bee against histological effects of Ochratoxin in vivo.
DOI:
https://doi.org/10.36320/ajb/v11.i2.8160Keywords:
Ochratoxin A, Lactic acid bacteria, Lactobacillus kunkeei,, AntioxidantsAbstract
Abstract
In this study One hundred honey bee worker were collected from two sites in AL-Diwanyia Governorate include Eastern Hamza and AL-Daghara city during April 2017. to detect antioxidant role of some bacteria isolated from honey bee gut workers against Ochratoxin.
Based on biochemical tests, the results show that out of one hundred samples of honey bee workers were screened, only Seventy isolates were isolated ,and the genera lactic acid bacteria ( Lactobacillus kunkeei ,lactobacillus plantrum and lactococus lactis) were highly abundant in honey bee gut . we examined protective role of Lactobacillus kunkeei in reduce histological effects of Ochratoxin A, the results showed treated animals with L. kunkeei. mixed with OTA showing not found any histopathological changes in liver and kidney.while treated animals with OTA only appear several pathological changes ,in kidney tissue this changes include :vascular congestion , dystrophy and the disappearance of the glomerulus in addition to cell necrosis of glomerulus.and cell necrosis, vascular congestion and loss of normal structure of liver.
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Angela, M.; Wolfgang, V.; Alexander, A.; Michael, K.; Paul, W.; Erwin, E.; Gordon, H. and Wolfgang, D. (2005).
Functional, biochemical, and pathological effects of repeated oral administration of Ochratoxin A to rats. Hem. Res. Toxicol., 18 (8): 1242-1252.
Asama ,T. H. ;Arima , T. ;Gomi , T. ;Keishi , H.; Tani , Y.; Kimura , T.; Tatefuji and Hashimoto,K. (2015). Lactobacillus kunkeei YB38 from honeybee products enhances IgA production in healthy adults. Journal Applied in Microbiology.Volume119, Issue3.
Asemi, Z.; Zare ,Z.; Shakeri, H.; Sabihi ,S.S.; Esmaillzadeh, A.(2013). Effect of multispecies probiotic supplements on metabolic profiles, hs-CRP, and oxidative stress in patients with type 2 diabetes. Ann. Nutr. Metab.; 63(1-2):1-9
Assaf ,H.; Azouri, H.; Pallardy, M.(2004) Ochratoxin A induces apoptosis in human lymphocytes through down regulation of Bcl-xL. Toxicol. Sci. ;79:35–344
Bancroft, J. D. and Stevens, A. (1982). Theory and practice of histological technique .Churchill living Stone. New York . pp.117
Bao, Y.; Wang, Z.; Zhang, Y.; Zhang, J.; Wang ,L.; Dong, X.; Su, F., Yao ,G.; Wang S.; Zhang H.(2012). Effect of Lactobacillus plantarum P-8 on lipid metabolism in hyperlipidemic rat model. Eur. J. Lipid Sci. Technol. ;114:1230–1236.
Benford ,D.;Boyle ,C.;Dekant,W.;Fuchs,E.;Gaylor,D.W.;Hard,G.,McGregory ,D.B.;Pitt,J.I.;Plestina,R.Shephard,G.;Solfrizzo,M.;Verger,P.J.(2001).Ochratoxin A safety Evolution Of certain Mycotoxins in food .WHO Food Additives 47.FAO Food and Nutrition Paper ,Vol.74.WHO Geneva,Switzerland ,pp.281-415.
Bennett and Klich, 2003J.(2003). Mycotoxins .Clin. Microbiol. Rev., 16 , pp. 497-516
Benson, H.J. (2002). '' Microbiological Application Laboratory Manual in General Microbiology '' .8thed .The the McGraw-Hill Companies.USA.
Brisse, S; Grimont, F; Grimont ,R., (2006) The genera Klebsiella: In Dworkin, M; Falkow, S; Rosenberg, E (Eds) The prokaryote (3rdEdition) Volume 6. Spinger-Verlag; New York, USA. pp 159-196.
Collee, J.G.; Fraser, A.G.; Marmion, B.P. and Simmons, A.; (1996). ''Practical Medical Microbiology''. Mackie & McMarteny. 14th.ed. Churchill Livingstone Inc., New York, U.S.A.
Dillon, R J; Vennard, C. T; Charnley, A. K. (2002) A note: gut bacteria produce components of a locust cohesion pheromone. J. Appl .Microbiol. ;92(4):759-63.
Dörrenhaus A.and Föllmann W. (1997)Effects of ochratoxin A on DNA repair in cultures of rat hepatocytes and porcine urinary bladder epithelial cells. Arch. Toxicol. ;71:709–713.
Feng, W; Wang, X. Q; Zhoa, W; Liu, G .Y; Wan, Y. J (2011) Isolation and characterization of lipase-producing bacteria in the intestineof the silkworm, Bombyx mori, reared on different forage. Journalof Insect Science 11(135): 1-9.
Gianni, B.; Anna, N. and Giuseppe, P. (2010). Effects of Ochratoxin A on Livestock Production. Toxins. 2:1796-1824.
Gilliam, M.;Valentine, D.K;Jane.Y.I (1997) Enterobacteriaceae isolated from foraging worker honey bees, Apis mellifera . J Invertebr Pathol 23: 38–41 Available
Halliwell,B.and Gutteridge ,J.M.C (1986).Iron and free radicals :Two aspects of antioxidant protection.Trends Biochem.Sci.11:372-375.
Hanif, N. ; Muhammad, G. ; Siddique , M. ; Khanum , A. ; Ahmed, T.; Gadahai , J. and Kaukab, G. (2008). Clinico-pathomorphological, serum biochemical and histological studies in broilers fed Ochratoxin A and a toxin deactivator. Br Poult. Sci., 49(5):632-42.
Hussein, H. S.; and Brasel, J. M. (2001). Toxicity, metabolism, and impact of mycotoxins on humans and animals. Toxicol., 167:101-134Inglis ,G.D; Sigler, L,.; Goette, M.S. (1998) Aerobic microorganisms associated with alfalfa leafcutter bees (Megachile rotundata). Microb Ecol 26: 125–143.
Kirkpatrick, L.A. and Feeney, B. C.(2013). A simple guide to IBM SPSS statistics for version 20.0. Student ed. Belmont, Calif: Wadsworth, Cengage Learning.
Lidia, M., and El-bieta, B.(2005). Identifiaction of lactobacillus and bifid bacterium species with PCR applied to quality control of fermented dairy beverages.. Pol. J. Food Nutr. Sci., Vol. 14/55, No 4, pp. 359–365
Lin M.Y. and Yen C.L.(1999) Antioxidative ability of lactic acid bacteria. J. Agric. Food Chem. ;47:1460–1466. .
Macffadin ,J.F.;(2000). '' Biochemical tests for identification of medical bacteria '' .3ed Ed., Lippincott Williams and Wilkins, USA.
Martarelli ,D.; Verdenelli ,M.C,.; Scuri, S.; Cocchioni, M.; Silvi, S.; Cecchini, C.; Pompei, P.(2011). Effect of a probiotic intake on oxidant and antioxidant parameters in plasma of athletes during intense exercise training. Curr Microbiol.; 62(6):1689-96.
Mohr, K.I. and Tebbe, C.C (2006) Diversity and phylotype consistency of bacteria in the guts of three bee species (Apoidea) at an oilseed rape field. Environ Microbiol 8: 258–272.
Peng ,X.L.; Xu ,W.T.;Wang ,Y.; Huang, K.L.; Liang, Z.H.; Zhao, W.W.; Luo ,Y.B. (2010).Mycotoxin ochratoxin A-induced cell death and changes in oxidative metabolism of Arabidopsis thaliana. Plant Cell Rep. ;29:153–161
Rad, A.H; Abbasalizadeh ,S.; Vazifekhah ,S.; Abbasalizadeh, F.; Hassanalilou, T.; Bastani, P.; Ejtahed, H.S.;Soroush, A.R.;Javadi ,M.; Mortazavian, A.M,.; Khalili, L.(2017) The Future of Diabetes Management by Healthy Probiotic Microorganisms. Curr Diabetes Rev.; 13(6):582-589.
Robbiano, L.; Carrozzino ,R.; Bacigalupo, M.; Corbu ,C.; Brambilla ,G.(2002) Correlation between induction of DNA fragmentation in urinary bladder cells from rats and humans and tissue-specific carcinogenic activity. Toxicology ; 179: 115-128.
Shen, Q.; Shang ,N.; Li P. (2011)In vitro and in vivo antioxidant activity of Bifidobacterium animalis 01 isolated from centenarians. Curr. Microbiol. ;62:1097–1103.
Wang, J.; Tang, H.; Zhang ,C.; Zhao, Y.; Derrien, M.; Rocher, E.; van-Hylckama Vlieg, J.E.; Strissel, K.; Zhao, L.; Obin, M.; Shen, J .(2015). Modulation of gut microbiota during probiotic-mediated attenuation of metabolic syndrome in high fat diet-fed mice. ISME J.; 9(1):1-15.
Wang, Y.; Wu, Y.; Wang, Y.; Fu, A.; Gong, L.; Li W.; Li Y. (2016) Bacillus amyloliquefaciens SC06 alleviates the oxidative stress of IPEC-1 via modulating Nrf2/Keap1 signaling pathway and decreasing ROS production. Appl. Microbiol. Biotechnol. ;101:1–12.
Wee, Y. J.; Kim, j. N. and Ryu, H. W. (2006). Biotechnology production of lactic acid bacteria and its recent applications. Food Tech. Biotechnol., 44(2): 161-172.
Zhang, Z. Q and Guan, C. Y (2009) Screening for lipase-producingEnterobacter agglomerans for biodiesel catalyzation. AfricanJournal of Biotechnology 8: 1273-1279.
Zocco, M.A.; dal Verme, L.Z.; Cremonini, F.; Piscaglia, A.C.; Nista, E.C.; Candelli M.; Novi, M.; Rigante, D.; Cazzato, I.A.; Ojetti, V.; Armuzzi, A.;Gasbarrini, G.; Gasbarrini, A.(2006) Efficacy of Lactobacillus GG in maintaining remission of ulcerative colitis. Aliment Pharmacol Ther. 1; 23(11):1567-
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