PCR detection of quorum sensing autoinducer (AI) type one among gram negative bacteria
DOI:
https://doi.org/10.36320/ajb/v13.i1.8136Abstract
Any bacterial species is found to be regulating their activities like pathogenicity, antibiotic resistant, biofilm production, and others by phenomenon system called quorum sensing. This system is dependent on production signaling molecules called autoinducers (AI); consequently, we tested the presence of QS genes by polymerase chain reaction (PCR) among different gram negative bacterial pathogens.
Material and Methods: Fifty isolates of each of Citrobacter spp., E. coli, Salmonella typhimurium, and Pseudomonas auroginosa were obtained from different private clinical laboratories in Baghdad and Al-Najaf cites in the period of June 2020- February 2021. Bacteria were identified using CHROM agar Orientation followed by Vitek2 system. Quorum sensing genes (abaI 382bp and AHL gene 498bp) were screened by PCR.
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Kher H.L., Krishnan T., Etchumanan V., Hong K., How K.Y., Lee L., and et al. (2019). Characterization of quorum sensing genes and N-acyl homoserine lactones in Citrobacter amalonaticus strain YG6. Gene; 684(5):58-69. DOI: https://doi.org/10.1016/j.gene.2018.10.031
Rasmussen, T. B. and Givskov, M. (2006). Quorum-sensing inhibitors as anti-pathogenic drugs. Int. J. Med. Microbiol.; 296: 149–161, doi: 10.1016/j.ijmm.02.005 DOI: https://doi.org/10.1016/j.ijmm.2006.02.005
Case R., Labbate M., and Kjelleberg S. (2008). AHL-driven quorum-sensing circuits: their frequency and function among the Proteobacteria. The ISME Journal; 2: 345–349 DOI: https://doi.org/10.1038/ismej.2008.13
Geske, G. D., O’Neill, J. C., and Blackwell, H. E. (2008). Expanding dialogues: from natural autoinducers to non-natural analogues that modulate quorum sensing in Gram-negative bacteria. Chem. Soc. Rev.; 37: 1432–1447, doi: 10.1039/B703021P. DOI: https://doi.org/10.1039/b703021p
Sullivan, M. J., Petty, N. K., andBeatson, S. A. (2011). Easyfig: a genome comparison visualizer. Bioinformatics; 27:1009–1010, doi: 10.1093/ bioinformatics/btr039 DOI: https://doi.org/10.1093/bioinformatics/btr039
Subramoni, S. and Venturi, V. (2009). LuxR-family ‘solos’: bachelor sensors/regulators of signalling molecules. Microbiology 155, 1377–1385, doi: 10.1099/mic.0.026849-0. DOI: https://doi.org/10.1099/mic.0.026849-0
Brameyer, S., Kresovic, D., Bode, H. B., and Heermann, R. (2015). Dialkylresorcinols as bacterial signaling molecules. Proc. Natl. Acad. Sci.; 112: 572–577, doi: 10.1073/pnas.1417685112. DOI: https://doi.org/10.1073/pnas.1417685112
Whitehead N.A., Barnard A.M.A., Slater H., Simpson N.J.L., and Salmond G.P.C. (2001). Quorum-sensing in Gram-negative bacteria. FEMS Microbiology Reviews; 25: 365-404. DOI: https://doi.org/10.1111/j.1574-6976.2001.tb00583.x
Surette M. G. and Bassler B. Quorum sensing in Escherichia coli and Salmonella typhimurium. Proc. Natl. Acad. Sci. USA Vol. 95 (1998): 7046–7050 DOI: https://doi.org/10.1073/pnas.95.12.7046
Deep, A., Chaudhary, U., and Gupta, V. (2011). Quorum sensing and Bacterial Pathogenicity: From Molecules to Disease. Journal of laboratory physicians, 3(1), 4–11. https://doi.org/10.4103/0974-2727.78553 DOI: https://doi.org/10.4103/0974-2727.78553
Goh S.Y., Khan S.A., Tee K.K., Abu Kasim N.H., Yin W.F. and Chan K.G. (2016) Quorum sensing activity of Citrobacter amalonaticus L8A, a bacterium isolated from dental plaque. Scientific Reports; 6:20702. DOI: 10.1038/srep20702. DOI: https://doi.org/10.1038/srep20702
Rizvi, M., Khan, F., Shukla, I., Malik, A., and Shaheen (2011). Rising prevalence of antimicrobial resistance in urinary tract infections during pregnancy: necessity for exploring newer treatment options. J. Lab. Physicians; 3: 98–103, doi: 10.4103/0974-2727.86842 DOI: https://doi.org/10.4103/0974-2727.86842
Yabaya, A. and Auta, B. (2007). Microorganisms associated with the urinogenital system of Vesico Vaginal Fistula (VVF) patients in north western Nigeria. Science World J.; 1(37). DOI: https://doi.org/10.4314/swj.v1i1.51697
Artero, A., Alberola, J., Eiros, J. M., Nogueira, J. M., and Cano, A. (2013). Pyelonephritis in pregnancy. How adequate is empirical treatment? Rev. Esp. Quimioter. 26: 30–33.
Surette M. G. and Bassler B.(1999). Regulation of autoinducer production in Salmonella typhimurium. Molecular Microbiology 31(2), 585–595. DOI: https://doi.org/10.1046/j.1365-2958.1999.01199.x
Miller S.T., Karina B. Xavier K.B., Campagna S.R., Taga M.E., Semmelhack M.F., Bassler B.L., Hughson F.M. (2004). Salmonella typhimurium Recognizes a Chemically Distinct Form of the Bacterial Quorum-Sensing Signal AI-2. Molecular Cell; 15(5):677-687 https://doi.org/10.1016/j.molcel.2004.07.020. DOI: https://doi.org/10.1016/j.molcel.2004.07.020
Kong K.F., Jayawardena S.R., Indulkar S.D., Puerto A., Koh C.L., Høiby N., and Mathee K. (2005). Pseudomonas aeruginosa AmpR Is a Global Transcriptional Factor That Regulates Expression of AmpC and PoxB -Lactamases, Proteases, Quorum Sensing, and Other Virulence Factors. Antimicrobial Agents and Chemotherapy; 49(11): 4567–4575. doi:10.1128/AAC.49.11.4567–4575. DOI: https://doi.org/10.1128/AAC.49.11.4567-4575.2005
Wang, Y., U. Ha, L. Zeng, and S. Jin. (2003). Regulation of membrane permeability by a two-component regulatory system in Pseudomonas aeruginosa. Antimicrob. Agents Chemother.; 47:95–101. DOI: https://doi.org/10.1128/AAC.47.1.95-101.2003
Oliver, A., R. Canton, P. Campo, F. Baquero, and J. Blazquez. (2000). High frequency of hypermutable Pseudomonas aeruginosa in cystic fibrosis lung infection. Science; 288:1251–1254 DOI: https://doi.org/10.1126/science.288.5469.1251
Fisher, J. F., S. O. Meroueh, and S. Mobashery. 2005. Bacterial resistance to beta-lactam antibiotics: compelling opportunism, compelling opportunity. Chem. Rev. 105:395–424 DOI: https://doi.org/10.1021/cr030102i
Aziz R.A. and Al-Jubori S. S. (2017). Characterization of Biofilm Production and Quorum Sensing Phenomenon among Antibiotic Resistant Acinetobacter Baumannii isolated From Wound in Fections in Iraq. Journal of Global Pharma Technology. 2017; 05(9):50-58.
Eberl, L. and Tümmler B. (2004). Pseudomonas aeruginosa and Burkholderia cepacia in cystic fibrosis: genome evolution, interactions and adaptation. Int. J. Med. Microbiol.; 294: 123–131, doi: 10.1016/j.ijmm.2004.06.022 DOI: https://doi.org/10.1016/j.ijmm.2004.06.022
Styles M.J., Early S.A., Tucholski T., West K.H.J., Ge Y., and Blackwell H.E. (2020). Chemical Control of Quorum Sensing in E. coli: Identification of Small Molecule Modulators of SdiA and Mechanistic Characterization of a Covalent Inhibitor ACS. Infect. Dis.; 6: 3092−3103 DOI: https://doi.org/10.1021/acsinfecdis.0c00654
Welsh, M. A., and Blackwell, H. E. (2016). Chemical probes of quorum sensing: from compound development to biological discovery. FEMS Microbiol Rev. 40 (5), 774−94. DOI: https://doi.org/10.1093/femsre/fuw009
Coulthurst, S. J. et al. (2007). Quorum sensing has an unexpected role in virulence in the model pathogen Citrobacter rodentium. EMBO Rep.; 8: 698–703, doi: 10.1038/sj.embor.7400984 DOI: https://doi.org/10.1038/sj.embor.7400984
Gnanendra S., Anusuya S. and Natarajan J. (2012). Molecular modeling and active site analysis of SdiA homolog, a putative quorum sensor for Salmonella typhimurium pathogenecity reveals specific binding patterns of AHL transcriptional regulators. Journal of Molecular Modeling 18: 4709–4719 DOI: https://doi.org/10.1007/s00894-012-1469-1
Nesse L., Berg K., Vestby L.K., Olsaker I., and Djønne B. (2011). Salmonella Typhimurium invasion of HEp-2 epithelial cells in vitro is increased by Nacylhomoserine lactone quorum sensing signals Live. Acta Veterinaria Scandinavica; 53:44:2-5. DOI: https://doi.org/10.1186/1751-0147-53-44
Frias, J., Olle, E. and Alsina (2001), M. Periodontal pathogens produce quorum sensing signal molecules. Infect. Immun. 69:3431–3434, doi: 10.1128/IAI.69.5.3431-3434. DOI: https://doi.org/10.1128/IAI.69.5.3431-3434.2001
Smith, R. S., and B. H. Iglewski. (2003). P. aeruginosa quorum-sensing systems and virulence. Curr. Opin. Microbiol. 6:56–60 DOI: https://doi.org/10.1016/S1369-5274(03)00008-0
Pearson JP and Pesci EC (1997). Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes. J. Bacteriol. 179:5756-5767. DOI: https://doi.org/10.1128/jb.179.18.5756-5767.1997
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