The Microorganisms associated domestic and imported meats and the possibility of controlling on it.

Abstract

This study was designed to isolate and diagnose the microorganisms associated with  imported and domestic meat depending on morphological , microscopically , biochemical and physiological properties as well as  PCR and vitek techniques. Toxic effect of metabolic compounds was produced by fungal isolate on physiological and biochemical parameters in albino rat males which were also determined in addition to the primary diagnosis for these compounds .In addition to that ,the evaluation of the effect of some natural and chemical compounds on occurrence of  these microorganisms in meat samples were investigated.

The results showed that a total number of microorganisms in samples of meat reached 222.71x10⁵ cfc/g in imported meat and 128.66 x 10⁵   cfc /g in domestic meat . Five bacterial isolates were isolated from meat samples were belong to Staphylococcus , Escherichia , Enterococcus , Salmonella  and Serratia  genera. Escherichia coli was high frequency and visible get to 89% and 28.94  % respectively ,while the frequency and visible of Staphylococcus aureus were 26.31 % and 70.3 % respectively , as well as the multiplex PCR results confirmed that  6 isolates genome belong to  E.coli were containing gene STX which is responsible for toxin synthesis .