Identification study for suspected women with Trichomonas vaginalis by PCR technique in AL-Najaf AL-Ashraf province
DOI:
https://doi.org/10.36320/ajb/v10.i1.8199Keywords:
Trichomonas vaginalis , PCR technique, Najaf ProvinceAbstract
The study was conducted on 450 out patients and 30 healthy women, whom have visited the department of infertility at Al-Sadder medical city and Al-Zahra Hospital in Najaf Province during the period from January till August, 2012. The infection with T.vaginalis in clinical suspected women determine by using PCR technique, the infection women numbers and percent by PCR test gave 58 and 12.88% respectively. DNA of T.vaginalis parasite was determined by PCR was using five specific primers (BTUB 9/2, BGL 1/2, TVA 5/6, TV 1/2 and OP 1/2) whereas the number, percent and sensitivity of infected women of these primers were 58 (12.88%) (100%), 56 (12.44%) (96.55%), 53 (11.77%) (91.37%) and 52 (11.55%) (89.65%) respectively, from comparing between these primers to evaluate the efficiency in diagnostic of T.vaginalis .Indicated that BTUB 9/2, BGL 1/2 the highest primers and OP 1/2 the lowest primers in diagnostic efficiency.
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References
Amany, A. A.; El-Aal, F. A. and Maha, H.E. (2008): Assessing feasibility of using samples for diagnosis of T.vaginilis by both wet mount and nested PCR. International Journal of Medicine, 1(4): 154-156.
Crucitti ,T.; Van, D. E. and Tehe, A., (2003): Comparison of Culture and different PCR assay for detection of Trichomonas vaginalis in self-collected vaginal swab specimens. Sex Transm infect; 79: 393 – 398.
Daron, C.E. (2009): Office laboratory diagnosis of vaginitis: clinician- performed tests compared with a rapid nucleic acid hybridization test available on line at (http: ̸ nd articles. Com ̸ P ̸ articles ̸ mi – m – m 0689 ̸ is – n6– v4lai -179136341), 02. 05.
Felix, D.B.; Okumura, C.Y.; Colocoussi, A. and Johnson, P.J. (2005). Trichomonas vaginalis Lipophosphoglycan mutants have Reduced Adherence and cytotoxicity to human Ectocervical cells. American society for Microbiology, 4(11): 1951 – 1958.
Johnson, R.E.; Green, T.A.; schachter, J.; Jones, R.B.; Hook, E.W.; Black, C.M.; Martin, D.H.; and Louis, M.E. (2000): Evaluation of nucleic acid amplification tests as reference test men. J Clin Microbial 38: 4382 – 4386.
Lubick, K.J. and Burg-ness, D.E. (2004): Purification and analysis of a phospholipase A-like Lytic factor of Trichomonas vaginalis. 72(3): 1284 – 1290.
Men-fang, S.; Pey-Ru, L. and Jah-Yao, L.I., (1997): Colorimetric One-Tube Nested PCR for Detection of Trichomonas vaginalis in vaginal Discharge. J. of Clinical. Microbiology. 35 (1): 132-138.
Shaio, M.F.; Lin, P.R. and Liu, J.Y. (1997): Colorimetric one - tube Nested PCR for detection of T. vaginalis in vaginal discharge, J. of clinical Microbiology, 35(1): 132-138.
Singh, S.; Singh, G.; Gautam, B.; Varadwaj, P. and Farmer, R. (2010): Trichomonas vaginalis genome analysis using bioinformatics approaches 3(1): 976 – 944.
Riley, D.E.; Roberis, M.C. and Takayama, T.(1992): Development of Polymerase Chain reaction-based diagnosis of Trichomonas vaginalis. J. Clin Microbiol, 30:465- 72.
Johnson, R.E.; Green, T.A.; schachter, J.; Jones, R.B.; Hook, E.W. ; Black, C.M.; Martin, D.H.; Louis, M.E. (2000). Evalution of nucleic acid amplification tests as reference test men. J Clin Microbiol 38: 4382 – 4386.
Stellrecht, K.A.; Woron, A.M. ; Mishrik, N.G. and venezia, R.A. (2004): comparison of multiplex PCR assay with culture for detection of genital mycoplasmas .J. Clin. Microbiol 42, 1528 – 1533.
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Copyright (c) 2018 Saleem Khteer Al-Hadraawy, Sami R. Al-Katib, Haithem M. H. Al-Awady
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