The correlation between some pathogenicity associated virulence factor and biofilm formation among uropathogenic Escherichia coli isolates in Al Najaf Al-Ashraf province
DOI:
https://doi.org/10.36320/ajb/v8.i2.9266Abstract
of each individual VF in separation from each other and studies of the assembly and function of UPEC virulence factors can offer a platform for the development of novel researches.
Aim of study: The study aim to investigate the incidence and prevalence of the some pathogenicity associated virulence factors (PAVF) in uropathogenic Escherichia coli (UPEC) such as motility (swarming and swimming ), hemolysin (α and β), siderophore and kind 1 fimbria and association of them with biofilm formation capability.
Patients and Method: A total of (170) urine specimens were collected during the period from May 2014 to November 2014 from patients suffering from UTI. All Patients were hospitalized in Alzahraa and Alsadr Teaching Hospitals.
Results: a) Out of 170 urine samples, only 70 (41.2% ) of it were UPEC positive isolates and 100 (58.8%) were negative isolates. b) 44 (63%) from cases showed mannose-sensitive haemagglutination (MSHA), 18 (26%) showed mannose-resistant haemagglutination (MRHA) and 8 (11%) shows negative results. c) 56(80%) of UPEC isolates were positive for motility and motility of both mode of motions (swarming and swimming ) the results were 66(94%) and 70(100%) respectively. d )16 (23%) of UPEC were β-hemolysis producer, 5 (7%) of it were α -hemolysis producer and 49(70%) did not have hemolysis action. e) 69(99%) were positive for siderophore production f) 67 (96%) of UPEC isolates has positive biofilm formation. g ) There were no significant values between biofilm production and the other virulence factors among the groups of UPEC isolates under investigation. As far as pearson correlation method, it was found that there was positive correlationship between biofilm production and virulence factors like ((hemolysin (α,β), siderophore and kind 1 fimbria )). On the other, there was negative correlationship between biofilm production and virulence factors like swarming and swimming (phenomenon).
Conclusion: In respect to the predominance occurrence of virulence factors either solely or collectively in UPEC strains this support the concept of association of UPEC with urinary pathogenicity.
Recommendation: future studies may be needed for identifying other pathogenic virulence factors among EPEC isolates necessary for emphasizing their pivotal roles in pathogenicity and to guide intervention to minimize its occurrence.
Key words: biofilm, virulence factor, UPEC.
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