The study of Oxidative stress, Sperm chromatin abnormality and seminal parameters in different groups for infertile patients.
DOI:
https://doi.org/10.36320/ajb/v8.i3.9302Keywords:
Sperm chromatin , Oxidative stress, infertile patientsAbstract
The study was carried out between January 2014 and October 2014 including one hundred and fifty five infertile men who attended the Fertility center in AL-Sadr Medical City. The Objective of this study was to study of oxidative stress, sperm chromatin abnormality and semen quality including ( semen volume, Liquefaction time, sperm concentration, motility, and normal morphology. The results of the study showed that there was a significant increase (p<0.05) in the semen volume, sperm concentration, progressive motility and normal morphology for unexplained compared to five abnormal semen groups. Also it was a significantly decrease (p<0.05) in liquefaction time, leukocyte concentration, MDA concentration and sperm chromatin abnormality for unexplained compared with abnormal semen groups. This study supports the conclusion that oxidative stress in seminal plasma is the main causes of sperm chromatin defect with defect of semen parameters and related with male infertility.
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Skakkebaek , N. E. ; Jorgensen , N. ; Main , K. M. ; Rajpert – DeMeyts , E. ; Leffers , H. ; Andersson , A. M. ; J , A. ; Carlsen , E. ; Mortensen , GK. ; Jensen , TK. And Toppari , J. (2006) : Is human fecundity declining ? International Journal of Andrology . 29(1) : 2-11 . DOI: https://doi.org/10.1111/j.1365-2605.2005.00573.x
Filipponi , D. and Feil , R. (2009) : Perturbation of genomic imprinting in oligozoospermia . Epigenetics . 4(1) : 27-30 . DOI: https://doi.org/10.4161/epi.4.1.7311
Hamada , A. ; Estevesb , S. C. and Agarwal , A. (2011) : Unexplained Male Infertility : Potential Causes and Management . Hum . Androl.,1: 2-16. DOI: https://doi.org/10.1097/01.XHA.0000397686.82729.09
Peivandi , S. ; Karimpour , A. and Moslemizade , N. (2010) : Effects of L- carnitine on infertile Men’s spermiogream , Radomized Clinical Trial . J. Reprod . Infertil . 10(4) : 331 .
Turchi , P. and Pescatori , E. S. (2005) : Is it necessary more anrology in infertility couple 2Ed . It . J. Sex. Reprod. Med. (12): 131-3 .
Esterhuizen , A. D. ; Franken , D. R. ; Lourens , J. C. H. ; Prinsloo , E. and VanRooyen . I. H. (2000) : Sperm chromatin packaging as an indicator of in – vitro fertilization rates , Human Reproduction . 15(3) : 657-661 . DOI: https://doi.org/10.1093/humrep/15.3.657
Roux , C. ; Tripogney , C. ; Joanne , C. etal. (2004) : Sperm chromatin Packaging as an indicator of in – vitro fertilization rates , Gynecologie Obstetrique et Fertilite , 32(9) : 792-798 . DOI: https://doi.org/10.1016/j.gyobfe.2004.06.014
Agarwal , A. and Said , T. M. (2003) : Role of sperm chromatin abnormalities and DNA damage in male infertility . Humal Reproduction Update.9(4) : 331-345 . DOI: https://doi.org/10.1093/humupd/dmg027
Talebi , A. R. ; Moein , M. R. ; Tabibnejad , N. and Gasemzadeh , J. (2008) : Effect of varicocel on chromatin condensation and DNA integrity of ejaculated spermatozoa using cytochemical test , Andrologia . 40(4) : 245-251 . DOI: https://doi.org/10.1111/j.1439-0272.2008.00852.x
Hekmatdoost , A. ; Lakpour , N. and Sadeghi , M. R. (2009) : Sperm chromatin integrity : etiologies and mechanisms of abnormality , assays clinical importance , preventing and repairing damage , Avicenna Journal of Medical Biotechnology , 1(3) : 147-160 .
Fortunato , A. ; Leo , R. ; Casale , S. ; Nacchia , G. ; Lignori , F. and Tosti , E. (2013) : Sperm DNA Fragmentation Assays Correlate With Sperm Abnormal Morphology and the pregnancy Outcome . JIVF Reprod Med Genet . 1: 101 . DOI: https://doi.org/10.4172/2375-4508.1000101
Shamsi , MB. ; Imam , SN. And Dada , R. (2011) : Sperm DNA Integrity Assays . Diagnostic and prognostic challenges and Implications in Management of Infertility . J Asist Reprod Genet , 28 : 1073-1085 . DOI: https://doi.org/10.1007/s10815-011-9631-8
Agarwal, A. ; Varghese , AC. And Sharma , RK. (2009) : Markers of oxidative stress and sperm chromatin integrity . Methods Mol. Biol. 590 : 377-420. DOI: https://doi.org/10.1007/978-1-60327-378-7_24
Desai, N. ; Sharma , R. ; Makker , K. ; Sabaneg , E. and Agrwal , A. (2009) : Physiologic levels of reactive oxygen species in neat semen of infertile men. Fertil. Steril. 92(5) : 1626-31 . DOI: https://doi.org/10.1016/j.fertnstert.2008.08.109
Agarwal, A. ; Makker , K. and Sharma , R. (2008) : Clinical revelance of oxidative stress in male factor infertility : an update . Am. J-Reprod . Immunol. 59(1) : 2-11 . DOI: https://doi.org/10.1111/j.1600-0897.2007.00559.x
World Health Organization (WHO)(1999): WHO Laboratory Manual for the examination of human semen and semen-cervical mucus interaction. 4th ed. New York. Cambridge University Press UK:4-59.
Shekarriz, M., Sharma, RK., Thomas, A.Jr and Agarwal, A. (1995): Positive myeloperoxidase staining (Endtz test) as an indicator of excessive reactive oxygen species formation in semen . J assist Reprod Genet, 12: 70-74. DOI: https://doi.org/10.1007/BF02211372
Muslish, R.K.; AL-Nimer, M. S. and AL-Zamely, o.M.(2002). The level of malondialdehyde after activation with (H2O2) and (CuSO4) and inhibition by desferoxamine and molsidomine in the serum of patients with acute myocardial infaraction. National Journal of chemistry; 5:139-148.
Sellami, A.; Nozha, C,; Soumay, B. Z.; Hanen, S.; Sahbi.; Tarek, R. and Rand Leila, K.(2013): Assessment of chromatin Abnormality in Human Spermatozoa : Useful Aniline Assay for Routine. DOI: https://doi.org/10.1155/2013/578631
Burger, R. (2013): Evaluation of spermatozoa and tests for an assisted reproductive techniques program: Corellation with semen parameters and ART outcome. M. Sc. Thesis, University of Stellenbsch.
Menkveld, R. Leukocytospermia. Int. Congr Ser (2004). 218-224. DOI: https://doi.org/10.1016/j.ics.2004.01.094
Saleh, R. A. and Agarwal, A. (2002): Oxidative stress and male infertility: From research bench to clinical practice. J. Androl., 23, 737-52. DOI: https://doi.org/10.1002/j.1939-4640.2002.tb02324.x
Aitken, R. J. and Baker, M. A. (2006): Oxidative stress, sperm survival and fertility control. Mol. And cell Endocrin. , 250, 66-69. DOI: https://doi.org/10.1016/j.mce.2005.12.026
Venkatesh, S. ; Singh, A. ; Shamsi, MB. ; Thilagavathi, J. ; Kumar, R. and etal., (2011): Clinical significance of sperm DNA damage threshold value in the assessment of male infertility. Reprod. Sci., 18: 1005-1013. DOI: https://doi.org/10.1177/1933719111401662
Sakkas, D. ; Mariethoz, E. ; Manicardi, G. ; Bizzaro, D. ; Bianchi, p. and Bianchi, U. (1999): Origin of DNA damage in ejaculated human spermatozoa. Rev. Reprod., 4: 31-7. DOI: https://doi.org/10.1530/ror.0.0040031
Ben Abdallah, F. ; Dammak, I. ; Attia, H. ; Hentati, B. and Ammar – Keskes, L. (2009): Lipid peroxidation and antioxidant enzyme activities in infertile men: correlation with semen parameter. J. Clin Lab Anal, 23: 99-104. DOI: https://doi.org/10.1002/jcla.20297
Agarwal, A. and Allamaneni, S. S. (2011): Free radicals and male reproduction. J. Indian Med Assoc, 109: 184-187.
Hsieh, YY. ; Chang, C. and Lin, CS (2006): Seminal malondialdehyde concentration but not glutathione peroxidase activity is negatively correlated with seminal concentration and motility. Int. J Biol. Sci., 2(1): 23. DOI: https://doi.org/10.7150/ijbs.2.23
Lakpour, N. ; Mahfouz, R. Z. ; Akhondi, M. M. ; Agarwal, A. ; Kharrzai, H. ; Zera'ati, H. ; Amirjannati, N. and Sadeghi, M. R. (2012): Relationship of seminal plasma antioxidants and serum male hormones with sperm chromatin status in male factor infertility. Systems Biology in Reproduction Medicine, 58: 236-244. DOI: https://doi.org/10.3109/19396368.2012.689923
Agarwal, A. and Allamaneni, S. S. R. (2004): Oxidants and antioxidants in human fertility. Mid East Fert. Society J. , 9, 187-49.
Said, T. M. ; Agarwal, A. ; Erenpreiss, J. ; Mahfouz, R. ; Giwereman, A. and Evenson, D. P. (2006): Association of early apoptosis with the nuclear integrity and abnormality in different seminal fractions. Cleveland , Malmo University, Malmo, Sweden.
Armstrong, J. S. ; Rajasekaran, M. ; Chamulitrat, W. ; Gatti, P. ; Hellstrom, W. J. and Sikka, S. C. (1999): Characterization of reactive oxygen species induced effects on human spermatozoa movement and energy metabolism . Free Radic Biol Med. , 26: 869-80. DOI: https://doi.org/10.1016/S0891-5849(98)00275-5
De Lamirand, E. and Cagon, C. (1992): Reactive oxygen species and human spermatozoa. Depletion of adenosine triphosphate plays an important role in the inhibition of sperm motility. J. Androl. , 3: 379-86. DOI: https://doi.org/10.1002/j.1939-4640.1992.tb03328.x
Kazerooni, T. ; Asadi, N. ; Jadid, L. ; Kazerooni, M. ; Ghanadi, A. ; Ghaffarpasand , F. ; Kazerooni, Y. and Zolghadr, J. (2009): Evaluation of sperm's chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion. J. Assist Reprod. Genet. , 26 (11): 591-596. DOI: https://doi.org/10.1007/s10815-009-9361-3
Hamad, M. F. (2014): Chromatin integrity in human ejaculate sperm of smokers and non – smoker patients and its relationship to seminal oxidative stress parameters. Int. J. Biosci. 4 (9): 126-41. DOI: https://doi.org/10.12692/ijb/4.9.126-141
Sepaniak, S. ; Forges, T. and Monnier – Barbarino, P. (2006): Cigarette smoking and fertility in women and men. Gyneco. Obstet. Fetil. , 34: 945-49.
Henkel, R. ; Kierspel, E. ; Stalf, T. and etal., (2005): Effect of reactive oxygen Species produced by spermatozoa and leukocytes on sperm functions in non Leukocytospermic patients . Fertil Steril. 83: 635-642. DOI: https://doi.org/10.1016/j.fertnstert.2004.11.022
Siegel, MS. (1993): The male infertility investigation and the role of the andrology laboratory. J. Reprod Med. 38: 317-334.
Wolff, H. ; Politich, J. A. ; Martinez, A. ; Haimovici, F. ; Hill. J. A. and Anderson, D. J. (1990): Leikocytospermia is associated with poor semen quality. Fertil. Steril., 53: 528-536. DOI: https://doi.org/10.1016/S0015-0282(16)53352-4
Aleksandra, K. ; Slawomir, K. and Ewa, B. (2004): Values of malondialdehyed in human seminal plasma. Progress in Med Res. , 2, 1-10.
Du Plessis, S. S. ; Gokul, S. and Agarwal, A. (2013): Semen hypervisosity: causes, consquences, and cures. Center for Reproductive Medicin, Cleveland Clinic, 5: 224-231. DOI: https://doi.org/10.2741/E610
Alkan, I. ; Semisek, F. ; Haklar, G. ; Kervancioglu, E. ; Ozveri, H. ; Yalcin, S. and Akdeas, A. (1997): Reactive oxygen species production bu the spermatozoa of patients with idiopathic infertility: relationship to seminal plasma antioxidants . J. Urol 157, 140-143. DOI: https://doi.org/10.1016/S0022-5347(01)65307-2
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Copyright (c) 2017 Mohsin K. AL-Murshdi, Yehya K. AL-Sultani
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