New development method for sulfamethoxazole Analysis in Pharmaceutical formulation using continuous Turbidity Flow Injection Technique

Abstract

This method uses a sensitive and novel methodology to accurately measure sulfamethoxazole in its pure and pharmaceutical form. The method is based on the formation of a white precipitate by the reaction of sulfamethoxazole and phosphomolybdic acid in an acidic medium. As a result of this reaction, the drug's interaction with the reagent is observed and measured using a home-made Ayah 6Sxl-T-2D Cell detector and turbidity flow injection. The ideal chemical parameters were optimized and chosen, including the phosphomolybdic acid concentration (0.5 mmol/L), acidity (acetic acid), and the flow rate (3.2, 2.8 ml/min) for the carrier and reagent streams, respectively, Sample volume as well as the delayed coil reaction were determined and adjusted. Sulfamethoxazole showed a linear range of (0.06-2.6 mmol/L) and a correlation coefficient of r = 0.9855. At the calibration graph's lowest concentration, the limit of detection (LOD) is (0.0141 mmol/L), and the limit of quantification (LOQ) is (0.0466 mmol/L).