Identification and Diagnosis of Cutaneous Leishmaninsis by Polymerase Chain Reaction Assay (Pcr) in Najaf Alashraf Province

Abstract

Objective: The present study aimed to determine the effectiveness of a polymerase chain reaction (PCR) technique for identification and differentiation of the cutaneous leishmaniasis parasite in clinical sample collected of lesion exudates patients.

Methodology: The (115) sample were examined by smear slid preparation culture on RPMI 1640 and NNN then DNA isolation. The DNA of the promastigote were amplified by PCR including primers selected on repetitive KDNA for identification of leishmania species. The data was analyzed by using frequency and percentage.

Results: The PCR result showed that two species of leishmania (L. major with 620 bp and L. tropica with 800 bp) exist.

Conclusion: It is concluded of the present study that the PCR technique has high specificity and sensitivity during the differentiation between Cutaneous Leishmanial species in comparing with conventional methods.

Recommendation: The PCR technique can be use it to diagnosis and differentiation between different type of Cutaneous Leishmaniasis.