Molecular Identification of Entamoeba histolytica Parasite by Using Actin and Amebapore-A Genes

Abstract

Objective: The present study was conducted in the province of Al- Najaf, for the period of April 2013 until November 2013 , which aims to identify the Entamoeba histolytica parasites by screening for Actin and Amebapore-A genes in stool samples by using the real-time PCR technique.

Methodology: The study included, collect ( 104 ) stool samples of patients with diarrhea arrivals to Al-Sadar Medical City Hospital, Al- Zahra Maternity and Children Hospital, Al-Manathera hospital and Al-Sajjad Hospital.

Results: The results of the real-time PCR revealed, the presence of E. histolytica parasites in (25) stool samples out of the (104) samples, the rate (24%).

Conclusion: In light of the results obtained through this study we concludes that the efficiency of real-time -PCR technique large and rapid in diagnosis of E. histolytica parasites in stool samples.

Recommendation: The study of gene expression of Actin and Amebapore-A genes, specific for E. histolytica parasite.