Effect of rapid freezing and thawing on Malondialdehyde concentration, abnormal sperm chromatin percent and sperm parameters of Asthenozoospermic patients

Abstract

Objective: The study aimed to study the effect of rapid freezing and thawing on Malondialdehyde concentration and abnormal sperm chromatin percent and sperm parameters of Asthenozoospermic patients.

Methodology: This study was performed in the laboratory of Intra cytoplasmic Injection and freezing of sperm in the
fertility center of Al-Sader Medical City / Al-najaf Alashraf City during the period of May, 2012 to April 2013, the study included 30 of Asthenozoospermic patients, the statistical analysis of this study performed by using
Student's t-test and paired t-test at the level of probability 0.05

Results: the results showed that is sperm rapid freezing process of patients who were Included in the study by used
sperm freezing media (Sperm Freeze) and doing the thawing processes after one month of freezing leads to a
significant decreasing (p<0.05) In the sperm concentration, sperm progressive motility percent, sperm viability
percent and normal sperm morphology percent. and a significant increasing (p<0.05). In The Malondialdehyde
concentration and abnormal Sperm chromatin percent and no significant difference (p<0.05) In round Cell concentration in comparison with their ranges after activation

Conclusion: It can be concluded in this study that sperm rapid freezing and thawing processes had a negative
effects on the semen and sperm parameters and Malondialdehyde concentration and abnormal sperm chromatin
percent In the all samples of study included patients.

Recommendation: Adding Anti oxidants to the sperm freezing media to decrease the negative effects of freezing
and increase the sperm parameters.