Evaluation of Peripheral Blood Lymphocytes as Cell Line for the Propagation of Human Herpes Simplex 1
Keywords:lymphocytes, cell culture, herpes simplex1, real-time PCR , fluorescent assay
Objectives: This study was planned to evaluate the use of peripheral blood lymphocytes as cell line for propagation of human herpes simplex 1, by the using of modern diagnostic techniques.
Methodology: Primarily, 40 samples were collected from dermal lesions, investigated by RT-PCR technique
directed to certify human herpes simplex1 infections Bosphore® HSV 1-2 Genotyping Kit v1(Anatolia
gene works, Turkey) was used for the detection protocol.
Results: the results revealed that HSV1 was correlated with 23(57.5%) of the total cases investigated. Five of HSV1
positive samples were selected and applied to the assay of in vitro studying of specific cytopathic effects(CPE) via
cell culture technique. Peripheral blood lymphocytes were isolated and propagated as a cell line. The demonstration
of specific HSV1 cytopathic effect was demonstrated by indirect immunofluorescent antibody technique. This
approach was revealed different degrees of sensitivity for supporting the growth of human herpes simplex1 virus;
these cells were sensitive enough to support the growth of HSV1 virus.
Conclusions: We concluded that Bosphore® HSV 1 Genotyping Kit v1allows very rapid detection of HSV DNA in
dermal leisions. peripheral blood lymphocytes were efficient enough for the studying of CPE of HSV1, while PCR
assay was more efficient and more précised as a diagnostic technique.
Recommendations: Real-time polymerase chain reaction is complementary to cell culture technique in diagnosis of
HSV, and it`s preferred to use specific primers of viral virulence factors and monitoring their pathogenicity.
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Copyright (c) 2015 Khalida Abbas Al-Kelaby
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