The Relevance of Adiponectin and Resistin Levels with Oxidative Stress in Insulin Resistant Type 2 Diabetes

Abstract

Objectives: The present study was designed to verify the changes of adiponectin and resistin with oxidative
stress in insulin resistant in diabetes mellitus type 2.

Methodology: To achieve this aim 60 patients with type 2 diabetes mellitus (24 male and 36 female ) of ages,
53.9 ± 12.4 years in addition to 30 apparently healthy individuals (13 males and 17 females) were enrolled.
The concentration of fasting blood glucose, triglyceride, insulin, adiponectin, resistin and malondialdehyde
(MDA) levels were measured. Fasting insulin concentration, adiponectin and resistin was measured by enzyme
linked immunosorbantassay( ELISA). Fasting blood glucose and triglyceride levels were determined by
enzymatic methods. Malondialdehyde (MDA) levels were measured by spectrophotometeric methods . Insulin
resistance was evaluated by four methods .

Results: The results indicated that out of 60 patients, 49 (81%), 45 (75%), 33 (55%) and 32 (53%) were
insulin resistant when they were evaluated by Homeostasis model assessment ( HOMA), Quantitative insulin
sensitivity check index (QUICKI), McAuley's index (MCA) and fasting insulin(FI) indices respectively. The 49
insulin resistant type 2 diabetic patients (IRP) that obtained through the HOMA method were assessed for the
alteration in the levels adiponectin, resistin and MDA. The analysis of the data revealed a significant decrease of
adiponectin in the IRP group when compared with those of the insulin sensitive patients (ISP) and the control
groups. Significant elevation of resistin levels were observed in the IRP group with respect to those of the
control groups.

Conclusion: Insulin resistance was found to be significantly positively correlated with resistin, MDA and
significantly negatively correlated with adiponectin levels in diabetic patients. Adiponectin exhibited
insignificant negative correlation with resistin and significant negative correlation with MDA levels, while the
level of resistin showed a significant positive correlation with MDA level in insulin resistant type 2 diabetics. 

Recommendations: A study on of the effect free fatty acid on the activity of insulin degrading enzymes in obese
individual.