Using Multiplex PCR Assay for Detection of Genital Mycoplasmasand Ureaplasmaspp in an Infertility Males.

Abstract

Objective: This study aim to diagnosis the types of bacteria Ureaplasmaspp and Mycoplasma hominis by
screening for multiple banding antigen (MBA) gene and 16S rRNA respectively in seminal fluid by using the
multiplex-PCR technique.

Methodology: The study included, a total (106) semen fluid samples, were collected of 86 infertile men
which have been diagnosed by specialized clinicians specialization in infertility and 20 other semen fluid
samples of healthy men used as control through six months (November 2013 until April 2014). All these
Date was analyzed by using spss (T-test) program All these samples were obtained of subjects who attended
to center infertility in medical city at Al-Sadar in Najaf province.

Results: The result of the multiplex-PCR revealed positive results in 13 (15.1%) of the 86 seminal fluid samples
from infertile patients which represented M. hominis 5(5.8%), U. urealyticum in 5 (5.8%) and U. parvum 3 (3.5%).

Conclusion: in light of the results obtained this study we concludes that the efficiency of multiplex-PCR large
and rapid in diagnosis of Ureaplasmaspp and Mycoplasma hominis in seminal fluid.

Recommendation: the study of detection of other microorganism (viruses, fungi, Chlamydia and Neisseria
gonorrhea) in men infertility.