Effect of Glutathione Adding to Freezing Media on Sperm Parameters and on Malondialdehyde Concentration and Abnormal Sperm Chromatin Percent of Oligozoospermic Patients

Sahib Yahia Almoreshedy; Wafak Gabory Mohammad; Hisham Qassim Mohammad Al-Nowainy

doi:10.36321/kjns.vi20143.2768

Authors

Sahib Yahia Almoreshedy University of Kufa
Wafak Gabory Mohammad University of Karbala
Hisham Qassim Mohammad Al-Nowainy University of Kufa

DOI:

https://doi.org/10.36321/kjns.vi20143.2768

Keywords:

Effect, Glutathione, Freezing Media, Sperm Parameters , Malondialdehyde , chromatin , Oligozoospermic

Abstract

Objective: The study aimed to study the effect antioxidant Glutathione adding to sperm Freezing Media ( Sperm Freeze) on Sperm Parameters and Malondialdehyde concentration and abnormal sperm chromatin percent of Oligozoospermic patients

Methodology: This study was performed in the laboratory of Intra cytoplasmic Injection and freezing of sperm in the fertility center of Al-Sader Medical City / Al-najaf Alashraf City during the period from May, 2012 to April 2013 , The study included 30 of Oligozoospermic patients, the statistical analysis of this study performed by using Student's t-test and paired t-test at the level of probability 0.05

Results: The study results shows that used of sperm Freezing Media ( Sperm Freeze) with adding of 1 mm from antioxidant Glutathione leads to a significant difference (p<0.05) in sperm parameters of study included patients, there was a significant increase (p<0.05) in the sperm progressive motility percent and sperm viability percent ,and there was a significant decrease (p<0.05) In The Malondialdehyde concentration and abnormal sperm chromatin percent and no significant difference (p<0.05) In the sperm concentration, normal sperm morphology percent and round cell concentration In comparison with their Means when used of sperm freezing media ( Sperm Freeze) alone.

Conclusion: It can be concluded from this study that sperm rapid freezing and thawing processes had a negative effect on the semen and sperm parameters and Malondialdehyde concentration and abnormal sperm chromatin percent in all samples of study included patients and adding of 1 mm from antioxidant Glutathione shows resistance to this negative effect leads to a significant increase (p<0.05) In the studying parameters .

Recommendation: using of antioxidant Glutathione with sperm Freezing Media (Sperm Freeze) in sperm cryopreservation processes