Evaluation of wet mount, Rapid antigen test and molecular techniques in the diagnosis of Trichomonas vaginalis in men.
DOI:
https://doi.org/10.36320/ajb/v16.i1.13669Keywords:
T. vaginalis, males, wet mount, RADT, RT-qPCRAbstract
Trichonas vaginalis is a sexual, human flagellated protozoan-obligated parasite. In Iraq, there are few studies concerning trichomoniasis in men and no studies were conducted on men in Maysan province. So this study was conducted to estimate the infection rate (IR) of T. vaginalis in males and evaluated four methods that used in diagnosis of this parasite including Wet mount, Rapid antigen detection test (RADT), Conventional polymerase chain reaction (CO-PCR), and Real-time quantitative polymerase chain reaction (RT-qPCR). Urine and semen samples were collected from 97 human males whose ages ranged from 18 to 50 years, and agreed to participate in the current study.
With the wet mount (WM), no one had been detected to be infected (0/97) with T. vaginalis. In RADT, it finds that 13.63%(6/44) of urine samples are positive for trichomoniasis by this test while all the semen samples gave negative results. Conventional PCR detected T. vaginalis in 18.18% (8/44) of urine samples and in 27.27% (12/44) of the semen sample. In Real-time quantitative PCR (RT-qPCR), T. vaginalis was detected in 29.54% (13/44) of urine samples and 31.82% (14/44) among the semen of the same males. The WM had the lower sensitivity of 0.0% and RT-qPCR had the highest sensitivity (100%) Finally, it can be concluded that the RT-qPCR is a golden standard tool for the diagnosis of T. vaginalis with high sensitivity and specificity and wet mount was poor and not recommended.
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